Accumulative difference image protocol for particle tracking in fluorescence microscopy tested in mouse lymphonodes.

The basic research in cell biology and in medical sciences makes large use of imaging tools mainly based on confocal fluorescence and, more recently, on non-linear excitation microscopy. Substantially the aim is the recognition of selected targets in the image and their tracking in time. We have dev...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Carlo E Villa, Michele Caccia, Laura Sironi, Laura D'Alfonso, Maddalena Collini, Ilaria Rivolta, Giuseppe Miserocchi, Tatiana Gorletta, Ivan Zanoni, Francesca Granucci, Giuseppe Chirico
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bd05f1afc6b246718dc12c2faecb4180
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bd05f1afc6b246718dc12c2faecb4180
record_format	dspace
spelling	oai:doaj.org-article:bd05f1afc6b246718dc12c2faecb41802021-11-18T06:35:59ZAccumulative difference image protocol for particle tracking in fluorescence microscopy tested in mouse lymphonodes.1932-620310.1371/journal.pone.0012216https://doaj.org/article/bd05f1afc6b246718dc12c2faecb41802010-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20808918/?tool=EBIhttps://doaj.org/toc/1932-6203The basic research in cell biology and in medical sciences makes large use of imaging tools mainly based on confocal fluorescence and, more recently, on non-linear excitation microscopy. Substantially the aim is the recognition of selected targets in the image and their tracking in time. We have developed a particle tracking algorithm optimized for low signal/noise images with a minimum set of requirements on the target size and with no a priori knowledge of the type of motion. The image segmentation, based on a combination of size sensitive filters, does not rely on edge detection and is tailored for targets acquired at low resolution as in most of the in-vivo studies. The particle tracking is performed by building, from a stack of Accumulative Difference Images, a single 2D image in which the motion of the whole set of the particles is coded in time by a color level. This algorithm, tested here on solid-lipid nanoparticles diffusing within cells and on lymphocytes diffusing in lymphonodes, appears to be particularly useful for the cellular and the in-vivo microscopy image processing in which few a priori assumption on the type, the extent and the variability of particle motions, can be done.Carlo E VillaMichele CacciaLaura SironiLaura D'AlfonsoMaddalena ColliniIlaria RivoltaGiuseppe MiserocchiTatiana GorlettaIvan ZanoniFrancesca GranucciGiuseppe ChiricoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 8, p e12216 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carlo E Villa Michele Caccia Laura Sironi Laura D'Alfonso Maddalena Collini Ilaria Rivolta Giuseppe Miserocchi Tatiana Gorletta Ivan Zanoni Francesca Granucci Giuseppe Chirico Accumulative difference image protocol for particle tracking in fluorescence microscopy tested in mouse lymphonodes.
description	The basic research in cell biology and in medical sciences makes large use of imaging tools mainly based on confocal fluorescence and, more recently, on non-linear excitation microscopy. Substantially the aim is the recognition of selected targets in the image and their tracking in time. We have developed a particle tracking algorithm optimized for low signal/noise images with a minimum set of requirements on the target size and with no a priori knowledge of the type of motion. The image segmentation, based on a combination of size sensitive filters, does not rely on edge detection and is tailored for targets acquired at low resolution as in most of the in-vivo studies. The particle tracking is performed by building, from a stack of Accumulative Difference Images, a single 2D image in which the motion of the whole set of the particles is coded in time by a color level. This algorithm, tested here on solid-lipid nanoparticles diffusing within cells and on lymphocytes diffusing in lymphonodes, appears to be particularly useful for the cellular and the in-vivo microscopy image processing in which few a priori assumption on the type, the extent and the variability of particle motions, can be done.
format	article
author	Carlo E Villa Michele Caccia Laura Sironi Laura D'Alfonso Maddalena Collini Ilaria Rivolta Giuseppe Miserocchi Tatiana Gorletta Ivan Zanoni Francesca Granucci Giuseppe Chirico
author_facet	Carlo E Villa Michele Caccia Laura Sironi Laura D'Alfonso Maddalena Collini Ilaria Rivolta Giuseppe Miserocchi Tatiana Gorletta Ivan Zanoni Francesca Granucci Giuseppe Chirico
author_sort	Carlo E Villa
title	Accumulative difference image protocol for particle tracking in fluorescence microscopy tested in mouse lymphonodes.
title_short	Accumulative difference image protocol for particle tracking in fluorescence microscopy tested in mouse lymphonodes.
title_full	Accumulative difference image protocol for particle tracking in fluorescence microscopy tested in mouse lymphonodes.
title_fullStr	Accumulative difference image protocol for particle tracking in fluorescence microscopy tested in mouse lymphonodes.
title_full_unstemmed	Accumulative difference image protocol for particle tracking in fluorescence microscopy tested in mouse lymphonodes.
title_sort	accumulative difference image protocol for particle tracking in fluorescence microscopy tested in mouse lymphonodes.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/bd05f1afc6b246718dc12c2faecb4180
work_keys_str_mv	AT carloevilla accumulativedifferenceimageprotocolforparticletrackinginfluorescencemicroscopytestedinmouselymphonodes AT michelecaccia accumulativedifferenceimageprotocolforparticletrackinginfluorescencemicroscopytestedinmouselymphonodes AT laurasironi accumulativedifferenceimageprotocolforparticletrackinginfluorescencemicroscopytestedinmouselymphonodes AT lauradalfonso accumulativedifferenceimageprotocolforparticletrackinginfluorescencemicroscopytestedinmouselymphonodes AT maddalenacollini accumulativedifferenceimageprotocolforparticletrackinginfluorescencemicroscopytestedinmouselymphonodes AT ilariarivolta accumulativedifferenceimageprotocolforparticletrackinginfluorescencemicroscopytestedinmouselymphonodes AT giuseppemiserocchi accumulativedifferenceimageprotocolforparticletrackinginfluorescencemicroscopytestedinmouselymphonodes AT tatianagorletta accumulativedifferenceimageprotocolforparticletrackinginfluorescencemicroscopytestedinmouselymphonodes AT ivanzanoni accumulativedifferenceimageprotocolforparticletrackinginfluorescencemicroscopytestedinmouselymphonodes AT francescagranucci accumulativedifferenceimageprotocolforparticletrackinginfluorescencemicroscopytestedinmouselymphonodes AT giuseppechirico accumulativedifferenceimageprotocolforparticletrackinginfluorescencemicroscopytestedinmouselymphonodes
_version_	1718424441294159872

Accumulative difference image protocol for particle tracking in fluorescence microscopy tested in mouse lymphonodes.

Ejemplares similares