Characteristics of acute diarrhea adult cases with samples positive for Norwalk virus

Remaining unmanageable,Norwalkvirus infection is clearly tended to be recorded at higher rate, including adult patients. In many cases, clinical picture of adult acute diarrhea in patients positive for Norwalk virus in clinical samples vs. pathogenetically-caused norovirus infection differs, thereby...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	E. A. Kozhukhova, I. V. Gorbova
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2019
Materias:	acute diarrhea culture method elisa pcr norwalk virus enteropathogen association Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/bd0823bb48f549d3ad96a671f3b1feed
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bd0823bb48f549d3ad96a671f3b1feed
record_format	dspace
spelling	oai:doaj.org-article:bd0823bb48f549d3ad96a671f3b1feed2021-11-22T07:09:53ZCharacteristics of acute diarrhea adult cases with samples positive for Norwalk virus2220-76192313-739810.15789/2220-7619-2019-2-375-380https://doaj.org/article/bd0823bb48f549d3ad96a671f3b1feed2019-07-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/659https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Remaining unmanageable,Norwalkvirus infection is clearly tended to be recorded at higher rate, including adult patients. In many cases, clinical picture of adult acute diarrhea in patients positive for Norwalk virus in clinical samples vs. pathogenetically-caused norovirus infection differs, thereby justifying comparison of clinical and laboratory data. A cohort retrospective study with 146 hospitalized adult patients suffering from acute moderate diarrhea positive for fecalNorwalkvirus was performed. Along with standard laboratory tests (culturing, serologic for detecting 4-fold increase in titer between paired serum samples, ELISA), detection of diarrhea-linked agents included PCR kit Amplisens® AII-bacto-screen-FL Lab (Interlabservice). The data obtained demonstrated that in adult patients with acute diarrhea 54.1% of cases were positive forNorwalkvirus as well as for other enteric pathogens, including bacteria found in 36.3% of cases. Moreover, clinical samples of patients with acute diarrhea hospitalized at least on day 4 vs. day 3 after the onset were significantly more often (by 1.5-fold) positive for association between Norwalk virus and other acute enteric infection agents mainly due to astrovirus (р = 0.03; PCR data) and Shigella spp. (culture-based and serologic methods; р = 0.03). In addition,Norwalkvirus was associated with rotavirus, but not other enteric pathogens, at 2.1-fold higher rate in clinical samples from patients treated vs. untreated with antimicrobials before hospitalization. Finally, clinical samples positive for Norwalk virus from patients with vs. without developed colitis syndrome were at higher risk of developing virus-bacterial enteric infection detecting Salmonella spp. at 7.6and 3-fold higher rate verified by culture-based and PCR assay, respectively. Importantly, patients with vs. without hemorrhagic colitis Salmonella spp. verified primarily a culture-based method was detected by 11-fold more frequently (p = 0.01).E. A. KozhukhovaI. V. GorbovaSankt-Peterburg : NIIÈM imeni Pasteraarticleacute diarrheaculture methodelisapcrnorwalk virusenteropathogen associationInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 9, Iss 2, Pp 375-380 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	acute diarrhea culture method elisa pcr norwalk virus enteropathogen association Infectious and parasitic diseases RC109-216
spellingShingle	acute diarrhea culture method elisa pcr norwalk virus enteropathogen association Infectious and parasitic diseases RC109-216 E. A. Kozhukhova I. V. Gorbova Characteristics of acute diarrhea adult cases with samples positive for Norwalk virus
description	Remaining unmanageable,Norwalkvirus infection is clearly tended to be recorded at higher rate, including adult patients. In many cases, clinical picture of adult acute diarrhea in patients positive for Norwalk virus in clinical samples vs. pathogenetically-caused norovirus infection differs, thereby justifying comparison of clinical and laboratory data. A cohort retrospective study with 146 hospitalized adult patients suffering from acute moderate diarrhea positive for fecalNorwalkvirus was performed. Along with standard laboratory tests (culturing, serologic for detecting 4-fold increase in titer between paired serum samples, ELISA), detection of diarrhea-linked agents included PCR kit Amplisens® AII-bacto-screen-FL Lab (Interlabservice). The data obtained demonstrated that in adult patients with acute diarrhea 54.1% of cases were positive forNorwalkvirus as well as for other enteric pathogens, including bacteria found in 36.3% of cases. Moreover, clinical samples of patients with acute diarrhea hospitalized at least on day 4 vs. day 3 after the onset were significantly more often (by 1.5-fold) positive for association between Norwalk virus and other acute enteric infection agents mainly due to astrovirus (р = 0.03; PCR data) and Shigella spp. (culture-based and serologic methods; р = 0.03). In addition,Norwalkvirus was associated with rotavirus, but not other enteric pathogens, at 2.1-fold higher rate in clinical samples from patients treated vs. untreated with antimicrobials before hospitalization. Finally, clinical samples positive for Norwalk virus from patients with vs. without developed colitis syndrome were at higher risk of developing virus-bacterial enteric infection detecting Salmonella spp. at 7.6and 3-fold higher rate verified by culture-based and PCR assay, respectively. Importantly, patients with vs. without hemorrhagic colitis Salmonella spp. verified primarily a culture-based method was detected by 11-fold more frequently (p = 0.01).
format	article
author	E. A. Kozhukhova I. V. Gorbova
author_facet	E. A. Kozhukhova I. V. Gorbova
author_sort	E. A. Kozhukhova
title	Characteristics of acute diarrhea adult cases with samples positive for Norwalk virus
title_short	Characteristics of acute diarrhea adult cases with samples positive for Norwalk virus
title_full	Characteristics of acute diarrhea adult cases with samples positive for Norwalk virus
title_fullStr	Characteristics of acute diarrhea adult cases with samples positive for Norwalk virus
title_full_unstemmed	Characteristics of acute diarrhea adult cases with samples positive for Norwalk virus
title_sort	characteristics of acute diarrhea adult cases with samples positive for norwalk virus
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2019
url	https://doaj.org/article/bd0823bb48f549d3ad96a671f3b1feed
work_keys_str_mv	AT eakozhukhova characteristicsofacutediarrheaadultcaseswithsamplespositivefornorwalkvirus AT ivgorbova characteristicsofacutediarrheaadultcaseswithsamplespositivefornorwalkvirus
_version_	1718417941092892672

Characteristics of acute diarrhea adult cases with samples positive for Norwalk virus

Ejemplares similares