Drug-loading capacity and nuclear targeting of multiwalled carbon nanotubes grafted with anionic amphiphilic copolymers

Hsieh-Chih Tsai,1,* Jeng-Yee Lin,2,* Faiza Maryani,1 Chun-Chiang Huang,1 Toyoko Imae1,31Graduate Institute of Applied Science and Technology, 2Division of Plastic Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei Medical University, 3Department of Chemical Engineering, Natio...

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Autores principales:	Tsai HC, Lin JY, Maryani F, Huang CC, Imae T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bd158dcb799644199f14e1ae90bdeddf
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spelling	oai:doaj.org-article:bd158dcb799644199f14e1ae90bdeddf2021-12-02T09:08:48ZDrug-loading capacity and nuclear targeting of multiwalled carbon nanotubes grafted with anionic amphiphilic copolymers1176-91141178-2013https://doaj.org/article/bd158dcb799644199f14e1ae90bdeddf2013-11-01T00:00:00Zhttp://www.dovepress.com/drug-loading-capacity-and-nuclear-targeting-of-multiwalled-carbon-nano-a15024https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Hsieh-Chih Tsai,1,* Jeng-Yee Lin,2,* Faiza Maryani,1 Chun-Chiang Huang,1 Toyoko Imae1,31Graduate Institute of Applied Science and Technology, 2Division of Plastic Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei Medical University, 3Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan *These authors contributed equally to this work Abstract: In this study, three types of hybrid nanotubes (NTs), ie, oxidized multiwalled carbon NTs (COOH MWCNTs), heparin (Hep)-conjugated MWCNTs (Hep MWCNTs), and diblock copolymer polyglycolic acid (PGA)-co-heparin conjugated to MWCNTs (PGA MWCNTs), were synthesized with improved biocompatibility and drug-loading capacity. Hydrophilic Hep substituents on MWCNTs improved biocompatibility and acted as nucleus-sensitive segments on the CNT carrier, whereas the addition of PGA enhanced drug-loading capacity. In the PGA MWCNT system, the amphiphilic copolymer (PGA-Hep) formed micelles on the side walls of CNTs, as confirmed by electron microscopy. The PGA system encapsulated the hydrophobic drug with high efficiency compared to the COOH MWCNT and Hep MWCNT systems. This is because the drug was loaded onto the PGA MWCNTs through hydrophobic forces and onto the CNTs by ∏–∏ stacking interactions. Additionally, most of the current drug-carrier designs that target cancer cells release the drug in the lysosome or cytoplasm. However, nuclear-targeted drug release is expected to kill cancer cells more directly and efficiently. In our study, PGA MWCNT carriers effectively delivered the active anticancer drug doxorubicin into targeted nuclei. This study may provide an effective strategy for the development of carbon-based drug carriers for nuclear-targeted drug delivery. Keywords: carbon nanotube, amphiphilic copolymer, drug loading, nucleus targeting, cancer therapyTsai HCLin JYMaryani FHuang CCImae TDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss Issue 1, Pp 4427-4440 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Tsai HC Lin JY Maryani F Huang CC Imae T Drug-loading capacity and nuclear targeting of multiwalled carbon nanotubes grafted with anionic amphiphilic copolymers
description	Hsieh-Chih Tsai,1,* Jeng-Yee Lin,2,* Faiza Maryani,1 Chun-Chiang Huang,1 Toyoko Imae1,31Graduate Institute of Applied Science and Technology, 2Division of Plastic Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei Medical University, 3Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan *These authors contributed equally to this work Abstract: In this study, three types of hybrid nanotubes (NTs), ie, oxidized multiwalled carbon NTs (COOH MWCNTs), heparin (Hep)-conjugated MWCNTs (Hep MWCNTs), and diblock copolymer polyglycolic acid (PGA)-co-heparin conjugated to MWCNTs (PGA MWCNTs), were synthesized with improved biocompatibility and drug-loading capacity. Hydrophilic Hep substituents on MWCNTs improved biocompatibility and acted as nucleus-sensitive segments on the CNT carrier, whereas the addition of PGA enhanced drug-loading capacity. In the PGA MWCNT system, the amphiphilic copolymer (PGA-Hep) formed micelles on the side walls of CNTs, as confirmed by electron microscopy. The PGA system encapsulated the hydrophobic drug with high efficiency compared to the COOH MWCNT and Hep MWCNT systems. This is because the drug was loaded onto the PGA MWCNTs through hydrophobic forces and onto the CNTs by ∏–∏ stacking interactions. Additionally, most of the current drug-carrier designs that target cancer cells release the drug in the lysosome or cytoplasm. However, nuclear-targeted drug release is expected to kill cancer cells more directly and efficiently. In our study, PGA MWCNT carriers effectively delivered the active anticancer drug doxorubicin into targeted nuclei. This study may provide an effective strategy for the development of carbon-based drug carriers for nuclear-targeted drug delivery. Keywords: carbon nanotube, amphiphilic copolymer, drug loading, nucleus targeting, cancer therapy
format	article
author	Tsai HC Lin JY Maryani F Huang CC Imae T
author_facet	Tsai HC Lin JY Maryani F Huang CC Imae T
author_sort	Tsai HC
title	Drug-loading capacity and nuclear targeting of multiwalled carbon nanotubes grafted with anionic amphiphilic copolymers
title_short	Drug-loading capacity and nuclear targeting of multiwalled carbon nanotubes grafted with anionic amphiphilic copolymers
title_full	Drug-loading capacity and nuclear targeting of multiwalled carbon nanotubes grafted with anionic amphiphilic copolymers
title_fullStr	Drug-loading capacity and nuclear targeting of multiwalled carbon nanotubes grafted with anionic amphiphilic copolymers
title_full_unstemmed	Drug-loading capacity and nuclear targeting of multiwalled carbon nanotubes grafted with anionic amphiphilic copolymers
title_sort	drug-loading capacity and nuclear targeting of multiwalled carbon nanotubes grafted with anionic amphiphilic copolymers
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/bd158dcb799644199f14e1ae90bdeddf
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Drug-loading capacity and nuclear targeting of multiwalled carbon nanotubes grafted with anionic amphiphilic copolymers

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