The effect of iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5.

One of the most important micronutrients for bacterial growth is iron, whose bioavailability in soil is limited. Consequently, rhizospheric bacteria such as Pseudomonas fluorescens employ a range of mechanisms to acquire or compete for iron. We investigated the transcriptomic and proteomic effects o...

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Autores principales:	Chee Kent Lim, Karl A Hassan, Sasha G Tetu, Joyce E Loper, Ian T Paulsen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bd15bd8e748243c7a1511048e9df87eb
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spelling	oai:doaj.org-article:bd15bd8e748243c7a1511048e9df87eb2021-11-18T07:15:09ZThe effect of iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5.1932-620310.1371/journal.pone.0039139https://doaj.org/article/bd15bd8e748243c7a1511048e9df87eb2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22723948/?tool=EBIhttps://doaj.org/toc/1932-6203One of the most important micronutrients for bacterial growth is iron, whose bioavailability in soil is limited. Consequently, rhizospheric bacteria such as Pseudomonas fluorescens employ a range of mechanisms to acquire or compete for iron. We investigated the transcriptomic and proteomic effects of iron limitation on P. fluorescens Pf-5 by employing microarray and iTRAQ techniques, respectively. Analysis of this data revealed that genes encoding functions related to iron homeostasis, including pyoverdine and enantio-pyochelin biosynthesis, a number of TonB-dependent receptor systems, as well as some inner-membrane transporters, were significantly up-regulated in response to iron limitation. Transcription of a ribosomal protein L36-encoding gene was also highly up-regulated during iron limitation. Certain genes or proteins involved in biosynthesis of secondary metabolites such as 2,4-diacetylphloroglucinol (DAPG), orfamide A and pyrrolnitrin, as well as a chitinase, were over-expressed under iron-limited conditions. In contrast, we observed that expression of genes involved in hydrogen cyanide production and flagellar biosynthesis were down-regulated in an iron-depleted culture medium. Phenotypic tests revealed that Pf-5 had reduced swarming motility on semi-solid agar in response to iron limitation. Comparison of the transcriptomic data with the proteomic data suggested that iron acquisition is regulated at both the transcriptional and post-transcriptional levels.Chee Kent LimKarl A HassanSasha G TetuJoyce E LoperIan T PaulsenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e39139 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chee Kent Lim Karl A Hassan Sasha G Tetu Joyce E Loper Ian T Paulsen The effect of iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5.
description	One of the most important micronutrients for bacterial growth is iron, whose bioavailability in soil is limited. Consequently, rhizospheric bacteria such as Pseudomonas fluorescens employ a range of mechanisms to acquire or compete for iron. We investigated the transcriptomic and proteomic effects of iron limitation on P. fluorescens Pf-5 by employing microarray and iTRAQ techniques, respectively. Analysis of this data revealed that genes encoding functions related to iron homeostasis, including pyoverdine and enantio-pyochelin biosynthesis, a number of TonB-dependent receptor systems, as well as some inner-membrane transporters, were significantly up-regulated in response to iron limitation. Transcription of a ribosomal protein L36-encoding gene was also highly up-regulated during iron limitation. Certain genes or proteins involved in biosynthesis of secondary metabolites such as 2,4-diacetylphloroglucinol (DAPG), orfamide A and pyrrolnitrin, as well as a chitinase, were over-expressed under iron-limited conditions. In contrast, we observed that expression of genes involved in hydrogen cyanide production and flagellar biosynthesis were down-regulated in an iron-depleted culture medium. Phenotypic tests revealed that Pf-5 had reduced swarming motility on semi-solid agar in response to iron limitation. Comparison of the transcriptomic data with the proteomic data suggested that iron acquisition is regulated at both the transcriptional and post-transcriptional levels.
format	article
author	Chee Kent Lim Karl A Hassan Sasha G Tetu Joyce E Loper Ian T Paulsen
author_facet	Chee Kent Lim Karl A Hassan Sasha G Tetu Joyce E Loper Ian T Paulsen
author_sort	Chee Kent Lim
title	The effect of iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5.
title_short	The effect of iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5.
title_full	The effect of iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5.
title_fullStr	The effect of iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5.
title_full_unstemmed	The effect of iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5.
title_sort	effect of iron limitation on the transcriptome and proteome of pseudomonas fluorescens pf-5.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/bd15bd8e748243c7a1511048e9df87eb
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The effect of iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5.

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