Trial Proteomic Qualitative and Quantitative Analysis of the Protein Matrix of Submandibular Sialoliths

Our studies aimed to explore the protein components of the matrix of human submandibular gland sialoliths. A qualitative analysis was carried out based on the filter aided sample preparation (FASP) methodology. In the protein extraction process, we evaluated the applicability of the standard deminer...

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Autores principales:	Paulina Czaplewska, Aleksandra E. Bogucka, Natalia Musiał, Dmitry Tretiakow, Andrzej Skorek, Dominik Stodulski
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	sialolith mass spectrometry proteomics FASP SWATH Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/bd16455eaaa24547b868ec81537dbeb2
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spelling	oai:doaj.org-article:bd16455eaaa24547b868ec81537dbeb22021-11-11T18:39:45ZTrial Proteomic Qualitative and Quantitative Analysis of the Protein Matrix of Submandibular Sialoliths10.3390/molecules262167251420-3049https://doaj.org/article/bd16455eaaa24547b868ec81537dbeb22021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6725https://doaj.org/toc/1420-3049Our studies aimed to explore the protein components of the matrix of human submandibular gland sialoliths. A qualitative analysis was carried out based on the filter aided sample preparation (FASP) methodology. In the protein extraction process, we evaluated the applicability of the standard demineralization step and the use of a lysis buffer containing sodium dodecyl sulfate (SDS) and dithiothreitol (DTT). The analysis of fragmentation spectra based on the human database allowed for the identification of 254 human proteins present in the deposits. In addition, the use of multi-round search in the PEAKS Studio program against the bacterial base allowed for the identification of 393 proteins of bacterial origin present in the extract obtained from sialolith, which so far has not been carried out for this biological material. Furthermore, we successfully applied the SWATH methodology, allowing for a relative quantitative analysis of human proteins present in deposits. The obtained results correlate with the classification of sialoliths proposed by Tretiakow. The performed functional analysis allowed for the first time the selection of proteins, the levels of which differ between the tested samples, which may suggest the role of these proteins in the calcification process in different types of sialoliths. These are preliminary studies, and drawing specific conclusions requires research on a larger group, but it provides us the basis for the continuation of the work that has already begun.Paulina CzaplewskaAleksandra E. BoguckaNatalia MusiałDmitry TretiakowAndrzej SkorekDominik StodulskiMDPI AGarticlesialolithmass spectrometryproteomicsFASPSWATHOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6725, p 6725 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sialolith mass spectrometry proteomics FASP SWATH Organic chemistry QD241-441
spellingShingle	sialolith mass spectrometry proteomics FASP SWATH Organic chemistry QD241-441 Paulina Czaplewska Aleksandra E. Bogucka Natalia Musiał Dmitry Tretiakow Andrzej Skorek Dominik Stodulski Trial Proteomic Qualitative and Quantitative Analysis of the Protein Matrix of Submandibular Sialoliths
description	Our studies aimed to explore the protein components of the matrix of human submandibular gland sialoliths. A qualitative analysis was carried out based on the filter aided sample preparation (FASP) methodology. In the protein extraction process, we evaluated the applicability of the standard demineralization step and the use of a lysis buffer containing sodium dodecyl sulfate (SDS) and dithiothreitol (DTT). The analysis of fragmentation spectra based on the human database allowed for the identification of 254 human proteins present in the deposits. In addition, the use of multi-round search in the PEAKS Studio program against the bacterial base allowed for the identification of 393 proteins of bacterial origin present in the extract obtained from sialolith, which so far has not been carried out for this biological material. Furthermore, we successfully applied the SWATH methodology, allowing for a relative quantitative analysis of human proteins present in deposits. The obtained results correlate with the classification of sialoliths proposed by Tretiakow. The performed functional analysis allowed for the first time the selection of proteins, the levels of which differ between the tested samples, which may suggest the role of these proteins in the calcification process in different types of sialoliths. These are preliminary studies, and drawing specific conclusions requires research on a larger group, but it provides us the basis for the continuation of the work that has already begun.
format	article
author	Paulina Czaplewska Aleksandra E. Bogucka Natalia Musiał Dmitry Tretiakow Andrzej Skorek Dominik Stodulski
author_facet	Paulina Czaplewska Aleksandra E. Bogucka Natalia Musiał Dmitry Tretiakow Andrzej Skorek Dominik Stodulski
author_sort	Paulina Czaplewska
title	Trial Proteomic Qualitative and Quantitative Analysis of the Protein Matrix of Submandibular Sialoliths
title_short	Trial Proteomic Qualitative and Quantitative Analysis of the Protein Matrix of Submandibular Sialoliths
title_full	Trial Proteomic Qualitative and Quantitative Analysis of the Protein Matrix of Submandibular Sialoliths
title_fullStr	Trial Proteomic Qualitative and Quantitative Analysis of the Protein Matrix of Submandibular Sialoliths
title_full_unstemmed	Trial Proteomic Qualitative and Quantitative Analysis of the Protein Matrix of Submandibular Sialoliths
title_sort	trial proteomic qualitative and quantitative analysis of the protein matrix of submandibular sialoliths
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bd16455eaaa24547b868ec81537dbeb2
work_keys_str_mv	AT paulinaczaplewska trialproteomicqualitativeandquantitativeanalysisoftheproteinmatrixofsubmandibularsialoliths AT aleksandraebogucka trialproteomicqualitativeandquantitativeanalysisoftheproteinmatrixofsubmandibularsialoliths AT nataliamusiał trialproteomicqualitativeandquantitativeanalysisoftheproteinmatrixofsubmandibularsialoliths AT dmitrytretiakow trialproteomicqualitativeandquantitativeanalysisoftheproteinmatrixofsubmandibularsialoliths AT andrzejskorek trialproteomicqualitativeandquantitativeanalysisoftheproteinmatrixofsubmandibularsialoliths AT dominikstodulski trialproteomicqualitativeandquantitativeanalysisoftheproteinmatrixofsubmandibularsialoliths
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Trial Proteomic Qualitative and Quantitative Analysis of the Protein Matrix of Submandibular Sialoliths

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