Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation

Abstract Glial cells such as astrocytes and oligodendrocytes play crucial roles in the central nervous system. To investigate the molecular mechanisms underlying the development and the biological functions of glial cells, simple and rapid techniques for glial cell-specific genetic manipulation in t...

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Autores principales:	Toshihide Hamabe-Horiike, Kanji Kawasaki, Masataka Sakashita, Chihiro Ishizu, Tomokazu Yoshizaki, Shin-ichi Harada, Keiko Ogawa-Ochiai, Yohei Shinmyo, Hiroshi Kawasaki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bd1999d6aafa4412ad581ae38ac3f70c
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spelling	oai:doaj.org-article:bd1999d6aafa4412ad581ae38ac3f70c2021-12-02T15:54:03ZGlial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation10.1038/s41598-021-84210-z2045-2322https://doaj.org/article/bd1999d6aafa4412ad581ae38ac3f70c2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84210-zhttps://doaj.org/toc/2045-2322Abstract Glial cells such as astrocytes and oligodendrocytes play crucial roles in the central nervous system. To investigate the molecular mechanisms underlying the development and the biological functions of glial cells, simple and rapid techniques for glial cell-specific genetic manipulation in the mouse cerebrum would be valuable. Here we uncovered that the Gfa2 promoter is suitable for selective gene expression in astrocytes when used with the piggyBac system and in utero electroporation. In contrast, the Blbp promoter, which has been used to induce astrocyte-specific gene expression in transgenic mice, did not result in astrocyte-specific gene expression. We also identified the Plp1 and Mbp promoters could be used with the piggyBac system and in utero electroporation to induce selective gene expression in oligodendrocytes. Furthermore, using our technique, neuron-astrocyte or neuron-oligodendrocyte interactions can be visualized by labeling neurons, astrocytes and oligodendrocytes differentially. Our study provides a fundamental basis for specific transgene expression in astrocytes and/or oligodendrocytes in the mouse cerebrum.Toshihide Hamabe-HoriikeKanji KawasakiMasataka SakashitaChihiro IshizuTomokazu YoshizakiShin-ichi HaradaKeiko Ogawa-OchiaiYohei ShinmyoHiroshi KawasakiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Toshihide Hamabe-Horiike Kanji Kawasaki Masataka Sakashita Chihiro Ishizu Tomokazu Yoshizaki Shin-ichi Harada Keiko Ogawa-Ochiai Yohei Shinmyo Hiroshi Kawasaki Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation
description	Abstract Glial cells such as astrocytes and oligodendrocytes play crucial roles in the central nervous system. To investigate the molecular mechanisms underlying the development and the biological functions of glial cells, simple and rapid techniques for glial cell-specific genetic manipulation in the mouse cerebrum would be valuable. Here we uncovered that the Gfa2 promoter is suitable for selective gene expression in astrocytes when used with the piggyBac system and in utero electroporation. In contrast, the Blbp promoter, which has been used to induce astrocyte-specific gene expression in transgenic mice, did not result in astrocyte-specific gene expression. We also identified the Plp1 and Mbp promoters could be used with the piggyBac system and in utero electroporation to induce selective gene expression in oligodendrocytes. Furthermore, using our technique, neuron-astrocyte or neuron-oligodendrocyte interactions can be visualized by labeling neurons, astrocytes and oligodendrocytes differentially. Our study provides a fundamental basis for specific transgene expression in astrocytes and/or oligodendrocytes in the mouse cerebrum.
format	article
author	Toshihide Hamabe-Horiike Kanji Kawasaki Masataka Sakashita Chihiro Ishizu Tomokazu Yoshizaki Shin-ichi Harada Keiko Ogawa-Ochiai Yohei Shinmyo Hiroshi Kawasaki
author_facet	Toshihide Hamabe-Horiike Kanji Kawasaki Masataka Sakashita Chihiro Ishizu Tomokazu Yoshizaki Shin-ichi Harada Keiko Ogawa-Ochiai Yohei Shinmyo Hiroshi Kawasaki
author_sort	Toshihide Hamabe-Horiike
title	Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation
title_short	Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation
title_full	Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation
title_fullStr	Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation
title_full_unstemmed	Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation
title_sort	glial cell type-specific gene expression in the mouse cerebrum using the piggybac system and in utero electroporation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bd1999d6aafa4412ad581ae38ac3f70c
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Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation

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