Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction
Abstract The presence of disease-specific antigens and autoantibodies in the sera of patients with atherosclerosis-related diseases has been widely reported and is considered to result from inflammation of the arterial wall and the involvement of immune factors. The aim of this study was to identify...
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Nature Portfolio
2021
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oai:doaj.org-article:bd1aa44625364c7b85c95ab9f37de29f2021-11-08T10:50:09ZSerum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction10.1038/s41598-021-01176-82045-2322https://doaj.org/article/bd1aa44625364c7b85c95ab9f37de29f2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01176-8https://doaj.org/toc/2045-2322Abstract The presence of disease-specific antigens and autoantibodies in the sera of patients with atherosclerosis-related diseases has been widely reported and is considered to result from inflammation of the arterial wall and the involvement of immune factors. The aim of this study was to identify a novel antibody in patients with ischemic stroke by serological identification of antigens using recombinant cDNA expression cloning from patients who had a transient ischemic attack (TIA). We identified the serpin peptidase inhibitor, clade E member 1 (SERPINE1), as a candidate antigen. The serum anti-SERPINE1 antibody levels quantified using amplified luminescent proximity homogeneous assay-linked immunosorbent assay were significantly higher in patients with ischemic stroke, including those with acute cerebral infarction (aCI), TIA, and chronic cerebral infarction, than in healthy donors. The antibody levels were strongly associated with old age, female sex, and presence of hypertension, diabetes mellitus, and cardiovascular disease. Age and intima-media thickness of the carotid artery were positively correlated with antibody levels, which suggests that SERPINE1 may reflect the progression of atherosclerosis. In a multivariate analysis, SERPINE1 antibody level was an independent predictor of aCI. Thus, the serum levels of anti-SERPINE1 antibody could potentially serve as a biomarker of atherothrombotic infarction.Masaaki KubotaYoichi YoshidaEiichi KobayashiTomoo MatsutaniShu-Yang LiBo-Shi ZhangSeiichiro MineToshio MachidaHirotaka TakizawaTakaki HiwasaYasuo IwadateNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
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Medicine R Science Q Masaaki Kubota Yoichi Yoshida Eiichi Kobayashi Tomoo Matsutani Shu-Yang Li Bo-Shi Zhang Seiichiro Mine Toshio Machida Hirotaka Takizawa Takaki Hiwasa Yasuo Iwadate Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction |
| description |
Abstract The presence of disease-specific antigens and autoantibodies in the sera of patients with atherosclerosis-related diseases has been widely reported and is considered to result from inflammation of the arterial wall and the involvement of immune factors. The aim of this study was to identify a novel antibody in patients with ischemic stroke by serological identification of antigens using recombinant cDNA expression cloning from patients who had a transient ischemic attack (TIA). We identified the serpin peptidase inhibitor, clade E member 1 (SERPINE1), as a candidate antigen. The serum anti-SERPINE1 antibody levels quantified using amplified luminescent proximity homogeneous assay-linked immunosorbent assay were significantly higher in patients with ischemic stroke, including those with acute cerebral infarction (aCI), TIA, and chronic cerebral infarction, than in healthy donors. The antibody levels were strongly associated with old age, female sex, and presence of hypertension, diabetes mellitus, and cardiovascular disease. Age and intima-media thickness of the carotid artery were positively correlated with antibody levels, which suggests that SERPINE1 may reflect the progression of atherosclerosis. In a multivariate analysis, SERPINE1 antibody level was an independent predictor of aCI. Thus, the serum levels of anti-SERPINE1 antibody could potentially serve as a biomarker of atherothrombotic infarction. |
| format |
article |
| author |
Masaaki Kubota Yoichi Yoshida Eiichi Kobayashi Tomoo Matsutani Shu-Yang Li Bo-Shi Zhang Seiichiro Mine Toshio Machida Hirotaka Takizawa Takaki Hiwasa Yasuo Iwadate |
| author_facet |
Masaaki Kubota Yoichi Yoshida Eiichi Kobayashi Tomoo Matsutani Shu-Yang Li Bo-Shi Zhang Seiichiro Mine Toshio Machida Hirotaka Takizawa Takaki Hiwasa Yasuo Iwadate |
| author_sort |
Masaaki Kubota |
| title |
Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction |
| title_short |
Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction |
| title_full |
Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction |
| title_fullStr |
Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction |
| title_full_unstemmed |
Serum anti-SERPINE1 antibody as a potential biomarker of acute cerebral infarction |
| title_sort |
serum anti-serpine1 antibody as a potential biomarker of acute cerebral infarction |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/bd1aa44625364c7b85c95ab9f37de29f |
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1718442657997389824 |