Analysis of biofilm bacterial communities under different shear stresses using size-fractionated sediment

Analysis of biofilm bacterial communities under different shear stresses using size-fractionated sediment

Abstract Microorganisms are ubiquitous in aqueous environments and are crucial for biogeochemical processes, but their community structures and functions remain poorly understood. In this paper, a rotating reactor was designed to study the effects of substrata and flow conditions on sediment bacteri...

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Autores principales: Hongwei Fang, Yishan Chen, Lei Huang, Guojian He
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/bd1c1d9c90fa4f23b191d0207d785396
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spelling oai:doaj.org-article:bd1c1d9c90fa4f23b191d0207d7853962021-12-02T12:32:04ZAnalysis of biofilm bacterial communities under different shear stresses using size-fractionated sediment10.1038/s41598-017-01446-42045-2322https://doaj.org/article/bd1c1d9c90fa4f23b191d0207d7853962017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01446-4https://doaj.org/toc/2045-2322Abstract Microorganisms are ubiquitous in aqueous environments and are crucial for biogeochemical processes, but their community structures and functions remain poorly understood. In this paper, a rotating reactor was designed to study the effects of substrata and flow conditions on sediment bacterial communities using 16S rRNA gene sequencing, assaying three groups of size-fractionated sediments and three different levels of applied shear stress. Proteobacteria, Firmicutes, and Bacteroidetes were the dominant phyla of the microbial communities, with more anaerobic bacteria and opportunistic pathogens being detected under static water conditions, while more aerobic bacteria were detected under dynamic water flow conditions. Most of the top 10 genera were present in all the samples; however, there were significant differences in the species abundance. Paludibacter and Comamonadaceae_unclassified were the most abundant genera under static and dynamic conditions, respectively. Under static water conditions, the medium-grained sediment had the highest microbial diversity, followed by the fine and coarse sediments. Under dynamic water flow conditions, a higher flow velocity corresponded to a greater microbial diversity. Overall, there was no significant difference in the community richness or diversity between the static and dynamic water flow conditions. This study is beneficial for further understanding the heterogeneities of microbial communities in natural aquatic ecosystems.Hongwei FangYishan ChenLei HuangGuojian HeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Hongwei Fang
Yishan Chen
Lei Huang
Guojian He
Analysis of biofilm bacterial communities under different shear stresses using size-fractionated sediment
description Abstract Microorganisms are ubiquitous in aqueous environments and are crucial for biogeochemical processes, but their community structures and functions remain poorly understood. In this paper, a rotating reactor was designed to study the effects of substrata and flow conditions on sediment bacterial communities using 16S rRNA gene sequencing, assaying three groups of size-fractionated sediments and three different levels of applied shear stress. Proteobacteria, Firmicutes, and Bacteroidetes were the dominant phyla of the microbial communities, with more anaerobic bacteria and opportunistic pathogens being detected under static water conditions, while more aerobic bacteria were detected under dynamic water flow conditions. Most of the top 10 genera were present in all the samples; however, there were significant differences in the species abundance. Paludibacter and Comamonadaceae_unclassified were the most abundant genera under static and dynamic conditions, respectively. Under static water conditions, the medium-grained sediment had the highest microbial diversity, followed by the fine and coarse sediments. Under dynamic water flow conditions, a higher flow velocity corresponded to a greater microbial diversity. Overall, there was no significant difference in the community richness or diversity between the static and dynamic water flow conditions. This study is beneficial for further understanding the heterogeneities of microbial communities in natural aquatic ecosystems.
format article
author Hongwei Fang
Yishan Chen
Lei Huang
Guojian He
author_facet Hongwei Fang
Yishan Chen
Lei Huang
Guojian He
author_sort Hongwei Fang
title Analysis of biofilm bacterial communities under different shear stresses using size-fractionated sediment
title_short Analysis of biofilm bacterial communities under different shear stresses using size-fractionated sediment
title_full Analysis of biofilm bacterial communities under different shear stresses using size-fractionated sediment
title_fullStr Analysis of biofilm bacterial communities under different shear stresses using size-fractionated sediment
title_full_unstemmed Analysis of biofilm bacterial communities under different shear stresses using size-fractionated sediment
title_sort analysis of biofilm bacterial communities under different shear stresses using size-fractionated sediment
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/bd1c1d9c90fa4f23b191d0207d785396
work_keys_str_mv AT hongweifang analysisofbiofilmbacterialcommunitiesunderdifferentshearstressesusingsizefractionatedsediment
AT yishanchen analysisofbiofilmbacterialcommunitiesunderdifferentshearstressesusingsizefractionatedsediment
AT leihuang analysisofbiofilmbacterialcommunitiesunderdifferentshearstressesusingsizefractionatedsediment
AT guojianhe analysisofbiofilmbacterialcommunitiesunderdifferentshearstressesusingsizefractionatedsediment
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