miR-F4-C12 Functions on the Regulation of Adipose Accumulation by Targeting PIK3R1 in Castrated Male Pigs

MicroRNAs (miRNAs) constitute small regulatory molecules for a wide array of biological activities (18~24 nucleotides in length), including adipogenesis and adipose deposition. Their effect is, however, incompletely defined in inducing fat accumulation in castrated male pigs. Based on our study, fou...

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Autores principales:	Qiao Xu, Jie Chen, Ximing Liu, Yabiao Luo, Tianzuo Wang, Meiying Fang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	pig microRNA adipose accumulation miR-F4-C12 PIK3R1 Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/bd1e57299bf248cfbd298069687b04ce
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spelling	oai:doaj.org-article:bd1e57299bf248cfbd298069687b04ce2021-11-25T16:14:13ZmiR-F4-C12 Functions on the Regulation of Adipose Accumulation by Targeting PIK3R1 in Castrated Male Pigs10.3390/ani111130532076-2615https://doaj.org/article/bd1e57299bf248cfbd298069687b04ce2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3053https://doaj.org/toc/2076-2615MicroRNAs (miRNAs) constitute small regulatory molecules for a wide array of biological activities (18~24 nucleotides in length), including adipogenesis and adipose deposition. Their effect is, however, incompletely defined in inducing fat accumulation in castrated male pigs. Based on our study, four nine-times miRNAs were selected to examine their functions in adipose formation activities. In 3T3-L1 cells and backfat tissues of castrated and intact male pigs, miR-F4-C12 was identified as a factor in adipose development utilizing quantitative real-time PCR (qRT-PCR). Further, miR-F4-C12 was identified to promote fat development, suggesting that miR-F4-C12 was involved in adipogenesis. Moreover, phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) was proposed by the TargetScan, miRDB and starBase as a target of miR-F4-C12 and verified through a two-luciferase reporter assay. The over-expression of miR-F4-C12 dramatically decreases the PIK3R1 protein level in 3T3-L1 cells. The mRNA and protein levels of PIK3R1 in castrated pigs are reduced relative to intact pigs, providing further evidence that PIK3R1 is involved in regulating adipose accumulation. These results suggest that miR-F4-C12 involves adipose development and may regulate subcutaneous adipose tissue accumulation by targeting PIK3R1 in castrated male pigs.Qiao XuJie ChenXiming LiuYabiao LuoTianzuo WangMeiying FangMDPI AGarticlepigmicroRNAadipose accumulationmiR-F4-C12PIK3R1Veterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3053, p 3053 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pig microRNA adipose accumulation miR-F4-C12 PIK3R1 Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	pig microRNA adipose accumulation miR-F4-C12 PIK3R1 Veterinary medicine SF600-1100 Zoology QL1-991 Qiao Xu Jie Chen Ximing Liu Yabiao Luo Tianzuo Wang Meiying Fang miR-F4-C12 Functions on the Regulation of Adipose Accumulation by Targeting PIK3R1 in Castrated Male Pigs
description	MicroRNAs (miRNAs) constitute small regulatory molecules for a wide array of biological activities (18~24 nucleotides in length), including adipogenesis and adipose deposition. Their effect is, however, incompletely defined in inducing fat accumulation in castrated male pigs. Based on our study, four nine-times miRNAs were selected to examine their functions in adipose formation activities. In 3T3-L1 cells and backfat tissues of castrated and intact male pigs, miR-F4-C12 was identified as a factor in adipose development utilizing quantitative real-time PCR (qRT-PCR). Further, miR-F4-C12 was identified to promote fat development, suggesting that miR-F4-C12 was involved in adipogenesis. Moreover, phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) was proposed by the TargetScan, miRDB and starBase as a target of miR-F4-C12 and verified through a two-luciferase reporter assay. The over-expression of miR-F4-C12 dramatically decreases the PIK3R1 protein level in 3T3-L1 cells. The mRNA and protein levels of PIK3R1 in castrated pigs are reduced relative to intact pigs, providing further evidence that PIK3R1 is involved in regulating adipose accumulation. These results suggest that miR-F4-C12 involves adipose development and may regulate subcutaneous adipose tissue accumulation by targeting PIK3R1 in castrated male pigs.
format	article
author	Qiao Xu Jie Chen Ximing Liu Yabiao Luo Tianzuo Wang Meiying Fang
author_facet	Qiao Xu Jie Chen Ximing Liu Yabiao Luo Tianzuo Wang Meiying Fang
author_sort	Qiao Xu
title	miR-F4-C12 Functions on the Regulation of Adipose Accumulation by Targeting PIK3R1 in Castrated Male Pigs
title_short	miR-F4-C12 Functions on the Regulation of Adipose Accumulation by Targeting PIK3R1 in Castrated Male Pigs
title_full	miR-F4-C12 Functions on the Regulation of Adipose Accumulation by Targeting PIK3R1 in Castrated Male Pigs
title_fullStr	miR-F4-C12 Functions on the Regulation of Adipose Accumulation by Targeting PIK3R1 in Castrated Male Pigs
title_full_unstemmed	miR-F4-C12 Functions on the Regulation of Adipose Accumulation by Targeting PIK3R1 in Castrated Male Pigs
title_sort	mir-f4-c12 functions on the regulation of adipose accumulation by targeting pik3r1 in castrated male pigs
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bd1e57299bf248cfbd298069687b04ce
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miR-F4-C12 Functions on the Regulation of Adipose Accumulation by Targeting PIK3R1 in Castrated Male Pigs

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