Silver-coated carbon nanotubes downregulate the expression of Pseudomonas aeruginosa virulence genes: a potential mechanism for their antimicrobial effect

Ejovwoke Dosunmu, Atul A Chaudhari, Shree R Singh, Vida A Dennis, Shreekumar R Pillai Center for NanoBiotechnology Research, Alabama State University, Montgomery, AL, USA Abstract: The antimicrobial activity of silver-coated carbon nanotubes (AgCNTs) and their potential mode of action against muco...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dosunmu E, Chaudhari AA, Singh SR, Dennis VA, Pillai SR
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bd271b2c1d8848ff8e25b55135be70bd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bd271b2c1d8848ff8e25b55135be70bd
record_format	dspace
spelling	oai:doaj.org-article:bd271b2c1d8848ff8e25b55135be70bd2021-12-02T06:38:00ZSilver-coated carbon nanotubes downregulate the expression of Pseudomonas aeruginosa virulence genes: a potential mechanism for their antimicrobial effect1178-2013https://doaj.org/article/bd271b2c1d8848ff8e25b55135be70bd2015-08-01T00:00:00Zhttp://www.dovepress.com/silver-coated-carbon-nanotubes-downregulate-the-expression-of-pseudomo-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ejovwoke Dosunmu, Atul A Chaudhari, Shree R Singh, Vida A Dennis, Shreekumar R Pillai Center for NanoBiotechnology Research, Alabama State University, Montgomery, AL, USA Abstract: The antimicrobial activity of silver-coated carbon nanotubes (AgCNTs) and their potential mode of action against mucoid and nonmucoid strains of Pseudomonas aeruginosa was investigated in vitro. The results showed that AgCNTs exhibited antimicrobial activity against both strains with minimum inhibitory concentrations of approximately 8 µg/mL, indicating a high sensitivity of P. aeruginosa to AgCNTs. AgCNTs were also bactericidal against both strains at the same minimum inhibitory concentration. Scanning and transmission electron-microscopy studies further revealed that a majority of the cells treated with AgCNTs transformed from smooth rod-shape morphology to disintegrated cells with broken/damaged membranes, resulting in leakage of cytoplasmic contents to produce ghost cells. The molecular effects of AgCNTs on P. aeruginosa genes involved in virulence and pathogenicity, stress response, and efflux pumps were evaluated for changes in their expression. Quantitative real-time PCR (qRT-PCR) showed that after exposure to AgCNTs, the expression levels of the rpoS, rsmZ, and oprD genes were significantly downregulated in both strains of P. aeruginosa compared to the untreated samples. These results suggest that the mechanism of action of AgCNTs may be attributed to their effect on cell-membrane integrity, downregulation of virulence-gene expression, and induction of general and oxidative stress in P. aeruginosa. Keywords: Pseudomonas aeruginosa, AgCNT, antimicrobial activity, qRT-PCRDosunmu EChaudhari AASingh SRDennis VAPillai SRDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 5025-5034 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Dosunmu E Chaudhari AA Singh SR Dennis VA Pillai SR Silver-coated carbon nanotubes downregulate the expression of Pseudomonas aeruginosa virulence genes: a potential mechanism for their antimicrobial effect
description	Ejovwoke Dosunmu, Atul A Chaudhari, Shree R Singh, Vida A Dennis, Shreekumar R Pillai Center for NanoBiotechnology Research, Alabama State University, Montgomery, AL, USA Abstract: The antimicrobial activity of silver-coated carbon nanotubes (AgCNTs) and their potential mode of action against mucoid and nonmucoid strains of Pseudomonas aeruginosa was investigated in vitro. The results showed that AgCNTs exhibited antimicrobial activity against both strains with minimum inhibitory concentrations of approximately 8 µg/mL, indicating a high sensitivity of P. aeruginosa to AgCNTs. AgCNTs were also bactericidal against both strains at the same minimum inhibitory concentration. Scanning and transmission electron-microscopy studies further revealed that a majority of the cells treated with AgCNTs transformed from smooth rod-shape morphology to disintegrated cells with broken/damaged membranes, resulting in leakage of cytoplasmic contents to produce ghost cells. The molecular effects of AgCNTs on P. aeruginosa genes involved in virulence and pathogenicity, stress response, and efflux pumps were evaluated for changes in their expression. Quantitative real-time PCR (qRT-PCR) showed that after exposure to AgCNTs, the expression levels of the rpoS, rsmZ, and oprD genes were significantly downregulated in both strains of P. aeruginosa compared to the untreated samples. These results suggest that the mechanism of action of AgCNTs may be attributed to their effect on cell-membrane integrity, downregulation of virulence-gene expression, and induction of general and oxidative stress in P. aeruginosa. Keywords: Pseudomonas aeruginosa, AgCNT, antimicrobial activity, qRT-PCR
format	article
author	Dosunmu E Chaudhari AA Singh SR Dennis VA Pillai SR
author_facet	Dosunmu E Chaudhari AA Singh SR Dennis VA Pillai SR
author_sort	Dosunmu E
title	Silver-coated carbon nanotubes downregulate the expression of Pseudomonas aeruginosa virulence genes: a potential mechanism for their antimicrobial effect
title_short	Silver-coated carbon nanotubes downregulate the expression of Pseudomonas aeruginosa virulence genes: a potential mechanism for their antimicrobial effect
title_full	Silver-coated carbon nanotubes downregulate the expression of Pseudomonas aeruginosa virulence genes: a potential mechanism for their antimicrobial effect
title_fullStr	Silver-coated carbon nanotubes downregulate the expression of Pseudomonas aeruginosa virulence genes: a potential mechanism for their antimicrobial effect
title_full_unstemmed	Silver-coated carbon nanotubes downregulate the expression of Pseudomonas aeruginosa virulence genes: a potential mechanism for their antimicrobial effect
title_sort	silver-coated carbon nanotubes downregulate the expression of pseudomonas aeruginosa virulence genes: a potential mechanism for their antimicrobial effect
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/bd271b2c1d8848ff8e25b55135be70bd
work_keys_str_mv	AT dosunmue silvercoatedcarbonnanotubesdownregulatetheexpressionofpseudomonasaeruginosavirulencegenesapotentialmechanismfortheirantimicrobialeffect AT chaudhariaa silvercoatedcarbonnanotubesdownregulatetheexpressionofpseudomonasaeruginosavirulencegenesapotentialmechanismfortheirantimicrobialeffect AT singhsr silvercoatedcarbonnanotubesdownregulatetheexpressionofpseudomonasaeruginosavirulencegenesapotentialmechanismfortheirantimicrobialeffect AT dennisva silvercoatedcarbonnanotubesdownregulatetheexpressionofpseudomonasaeruginosavirulencegenesapotentialmechanismfortheirantimicrobialeffect AT pillaisr silvercoatedcarbonnanotubesdownregulatetheexpressionofpseudomonasaeruginosavirulencegenesapotentialmechanismfortheirantimicrobialeffect
_version_	1718399836155281408

Silver-coated carbon nanotubes downregulate the expression of Pseudomonas aeruginosa virulence genes: a potential mechanism for their antimicrobial effect

Ejemplares similares