Intraperitoneal Injection of Graphene Oxide Nanoparticle Accelerates Stem Cell Therapy Effects on Acute Kidney Injury

Tahereh Foroutan,1 Mohsen Nafar,2 Elaheh Motamedi3 1Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran; 2Nephrology Department of Erfan Hospital, Tehran, Iran; 3Department of Nanotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), A...

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Autores principales:	Foroutan T, Nafar M, Motamedi E
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	grapheme oxide kidney injury stem cell Cytology QH573-671
Acceso en línea:	https://doaj.org/article/bd2949bf473649e884f618445a809722
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spelling	oai:doaj.org-article:bd2949bf473649e884f618445a8097222021-12-02T06:22:00ZIntraperitoneal Injection of Graphene Oxide Nanoparticle Accelerates Stem Cell Therapy Effects on Acute Kidney Injury1178-6957https://doaj.org/article/bd2949bf473649e884f618445a8097222020-02-01T00:00:00Zhttps://www.dovepress.com/intraperitoneal-injection-of-graphene-oxide-nanoparticle-accelerates-s-peer-reviewed-article-SCCAAhttps://doaj.org/toc/1178-6957Tahereh Foroutan,1 Mohsen Nafar,2 Elaheh Motamedi3 1Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran; 2Nephrology Department of Erfan Hospital, Tehran, Iran; 3Department of Nanotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranCorrespondence: Tahereh ForoutanKharazmi University, Mofateh Street, Tehran 15614, IranTel/Fax +98 21 86072709Email foroutan@khu.ac.irPurpose: Graphene-based nanostructures have shown some degree of stem cell protection against cell death. Acute kidney injury (AKI) is a major cause of mortality in hospitalized patients. Here, graphene oxide (GO) was used to improve the efficacy of bone marrow-derived mesenchymal stem cells (MSCs) in the treatment of AKI induced by cisplatin, a chemotherapy medication used to treat a number of cancers.Materials and Methods: Cisplatin-induced AKI was modeled in male rats. Intraperitoneal injection of MSCs mixed with GO, synthesized by graphite powder, H2SO4, and KMnO4 was administered in modeled animals. Biochemical analysis of serum and histological and immunohistochemical (IHC) staining of kidney tissue samples were determined.Results: Administration of GO nanoparticles suspended in MSCs reduced serum levels of creatinine (Cr) and blood urea nitrogen (BUN) in cisplatin-induced AKI in the experimental group compared to the control group. Histopathological evaluation also showed an improvement of morphological alterations of kidney, such as cellular proliferation, apoptosis and necrosis, cyst formation and intratubular debris in the experimental group compared to the control group. Our data revealed that GO injection alone without MSCs accelerated the improvement of the kidney injury induced by cisplatin.Conclusion: This study demonstrated that suspended GO could enhance the efficacy of stem cells in the treatment of AKI. GO alone without stem cell accelerates the improvement of cisplatin-induced AKI.Keywords: graphene oxide, kidney injury, stem cellForoutan TNafar MMotamedi EDove Medical Pressarticlegrapheme oxidekidney injurystem cellCytologyQH573-671ENStem Cells and Cloning: Advances and Applications, Vol Volume 13, Pp 21-32 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	grapheme oxide kidney injury stem cell Cytology QH573-671
spellingShingle	grapheme oxide kidney injury stem cell Cytology QH573-671 Foroutan T Nafar M Motamedi E Intraperitoneal Injection of Graphene Oxide Nanoparticle Accelerates Stem Cell Therapy Effects on Acute Kidney Injury
description	Tahereh Foroutan,1 Mohsen Nafar,2 Elaheh Motamedi3 1Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran; 2Nephrology Department of Erfan Hospital, Tehran, Iran; 3Department of Nanotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranCorrespondence: Tahereh ForoutanKharazmi University, Mofateh Street, Tehran 15614, IranTel/Fax +98 21 86072709Email foroutan@khu.ac.irPurpose: Graphene-based nanostructures have shown some degree of stem cell protection against cell death. Acute kidney injury (AKI) is a major cause of mortality in hospitalized patients. Here, graphene oxide (GO) was used to improve the efficacy of bone marrow-derived mesenchymal stem cells (MSCs) in the treatment of AKI induced by cisplatin, a chemotherapy medication used to treat a number of cancers.Materials and Methods: Cisplatin-induced AKI was modeled in male rats. Intraperitoneal injection of MSCs mixed with GO, synthesized by graphite powder, H2SO4, and KMnO4 was administered in modeled animals. Biochemical analysis of serum and histological and immunohistochemical (IHC) staining of kidney tissue samples were determined.Results: Administration of GO nanoparticles suspended in MSCs reduced serum levels of creatinine (Cr) and blood urea nitrogen (BUN) in cisplatin-induced AKI in the experimental group compared to the control group. Histopathological evaluation also showed an improvement of morphological alterations of kidney, such as cellular proliferation, apoptosis and necrosis, cyst formation and intratubular debris in the experimental group compared to the control group. Our data revealed that GO injection alone without MSCs accelerated the improvement of the kidney injury induced by cisplatin.Conclusion: This study demonstrated that suspended GO could enhance the efficacy of stem cells in the treatment of AKI. GO alone without stem cell accelerates the improvement of cisplatin-induced AKI.Keywords: graphene oxide, kidney injury, stem cell
format	article
author	Foroutan T Nafar M Motamedi E
author_facet	Foroutan T Nafar M Motamedi E
author_sort	Foroutan T
title	Intraperitoneal Injection of Graphene Oxide Nanoparticle Accelerates Stem Cell Therapy Effects on Acute Kidney Injury
title_short	Intraperitoneal Injection of Graphene Oxide Nanoparticle Accelerates Stem Cell Therapy Effects on Acute Kidney Injury
title_full	Intraperitoneal Injection of Graphene Oxide Nanoparticle Accelerates Stem Cell Therapy Effects on Acute Kidney Injury
title_fullStr	Intraperitoneal Injection of Graphene Oxide Nanoparticle Accelerates Stem Cell Therapy Effects on Acute Kidney Injury
title_full_unstemmed	Intraperitoneal Injection of Graphene Oxide Nanoparticle Accelerates Stem Cell Therapy Effects on Acute Kidney Injury
title_sort	intraperitoneal injection of graphene oxide nanoparticle accelerates stem cell therapy effects on acute kidney injury
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/bd2949bf473649e884f618445a809722
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Intraperitoneal Injection of Graphene Oxide Nanoparticle Accelerates Stem Cell Therapy Effects on Acute Kidney Injury

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