Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion
Objectives: The present study aimed to assess the expression of caspase-1 and caspase-1-dependent processing of cytokines, such as interleukin (IL)-1β and IL-18, in the middle ear effusion of children with otitis media with effusion (OME) in order to identify the potential role of inflammasomes in O...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:bd2d2aa0bf6245e380887d4151a277342021-11-05T11:07:18ZEvaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion2296-236010.3389/fped.2021.732973https://doaj.org/article/bd2d2aa0bf6245e380887d4151a277342021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fped.2021.732973/fullhttps://doaj.org/toc/2296-2360Objectives: The present study aimed to assess the expression of caspase-1 and caspase-1-dependent processing of cytokines, such as interleukin (IL)-1β and IL-18, in the middle ear effusion of children with otitis media with effusion (OME) in order to identify the potential role of inflammasomes in OME.Methods: This study included 29 children scheduled for myringotomy with the insertion of tympanostomy tubes due to OME. Middle ear effusion (MEE) was collected during the surgery. Caspase-1, IL-1β, and IL-18 were assayed using enzyme-linked immunosorbent assay kits. The levels were compared between those with mucoid and serous MEE and those with and without a history of ventilation tube insertion.Results: Caspase-1, IL-1β, and IL-18 were detected in all samples. The caspase-1, IL-1β, and IL-18 levels did not significantly differ between mucoid samples and serous samples. No statistical significances were discovered in caspase-1, IL-1β, and IL-18 levels between with and without a history of ventilation tube groups. There was a significant negative correlation between IL-1β and IL-18 and the duration of OME (p < 0.05). However, no significant correlation was found between caspase-1 and disease duration.Conclusions: Inflammasomes may participate in the inflammatory process of OME. IL-1β and IL-18 levels in the MEE decreased over time.Shanshan LiuShanshan LiuLining GuoLining GuoMin ChenMin ChenWei LiuWei LiuYanhong LiYanhong LiXiaoxu WangXiaoxu WangShilan LiShilan LiJie ZhangJie ZhangXin NiXin NiFrontiers Media S.A.articleotitis media with effusioncaspase-1interleukin-1βinterleukin-18inflammasomeschildrenPediatricsRJ1-570ENFrontiers in Pediatrics, Vol 9 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
otitis media with effusion caspase-1 interleukin-1β interleukin-18 inflammasomes children Pediatrics RJ1-570 |
| spellingShingle |
otitis media with effusion caspase-1 interleukin-1β interleukin-18 inflammasomes children Pediatrics RJ1-570 Shanshan Liu Shanshan Liu Lining Guo Lining Guo Min Chen Min Chen Wei Liu Wei Liu Yanhong Li Yanhong Li Xiaoxu Wang Xiaoxu Wang Shilan Li Shilan Li Jie Zhang Jie Zhang Xin Ni Xin Ni Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion |
| description |
Objectives: The present study aimed to assess the expression of caspase-1 and caspase-1-dependent processing of cytokines, such as interleukin (IL)-1β and IL-18, in the middle ear effusion of children with otitis media with effusion (OME) in order to identify the potential role of inflammasomes in OME.Methods: This study included 29 children scheduled for myringotomy with the insertion of tympanostomy tubes due to OME. Middle ear effusion (MEE) was collected during the surgery. Caspase-1, IL-1β, and IL-18 were assayed using enzyme-linked immunosorbent assay kits. The levels were compared between those with mucoid and serous MEE and those with and without a history of ventilation tube insertion.Results: Caspase-1, IL-1β, and IL-18 were detected in all samples. The caspase-1, IL-1β, and IL-18 levels did not significantly differ between mucoid samples and serous samples. No statistical significances were discovered in caspase-1, IL-1β, and IL-18 levels between with and without a history of ventilation tube groups. There was a significant negative correlation between IL-1β and IL-18 and the duration of OME (p < 0.05). However, no significant correlation was found between caspase-1 and disease duration.Conclusions: Inflammasomes may participate in the inflammatory process of OME. IL-1β and IL-18 levels in the MEE decreased over time. |
| format |
article |
| author |
Shanshan Liu Shanshan Liu Lining Guo Lining Guo Min Chen Min Chen Wei Liu Wei Liu Yanhong Li Yanhong Li Xiaoxu Wang Xiaoxu Wang Shilan Li Shilan Li Jie Zhang Jie Zhang Xin Ni Xin Ni |
| author_facet |
Shanshan Liu Shanshan Liu Lining Guo Lining Guo Min Chen Min Chen Wei Liu Wei Liu Yanhong Li Yanhong Li Xiaoxu Wang Xiaoxu Wang Shilan Li Shilan Li Jie Zhang Jie Zhang Xin Ni Xin Ni |
| author_sort |
Shanshan Liu |
| title |
Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion |
| title_short |
Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion |
| title_full |
Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion |
| title_fullStr |
Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion |
| title_full_unstemmed |
Evaluation of Caspase-1, Interleukin-1β, and Interleukin-18, in the Middle Ear Effusion in Children With Otitis Media With Effusion |
| title_sort |
evaluation of caspase-1, interleukin-1β, and interleukin-18, in the middle ear effusion in children with otitis media with effusion |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/bd2d2aa0bf6245e380887d4151a27734 |
| work_keys_str_mv |
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