Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme

RNA sequence-structure-function relationships can be studied via assays of variant activity. The authors developed a method to systematically determine ribozyme variant kinetics, using it to map double-mutant interactions and show that cleavage rate drives sequence conservation in the glmS ribozyme.

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Detalles Bibliográficos
Autores principales: Johan O. L. Andreasson, Andrew Savinov, Steven M. Block, William J. Greenleaf
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/bd2de6930e9143e08b42ce2dd005876a
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Descripción
Sumario:RNA sequence-structure-function relationships can be studied via assays of variant activity. The authors developed a method to systematically determine ribozyme variant kinetics, using it to map double-mutant interactions and show that cleavage rate drives sequence conservation in the glmS ribozyme.