Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme

RNA sequence-structure-function relationships can be studied via assays of variant activity. The authors developed a method to systematically determine ribozyme variant kinetics, using it to map double-mutant interactions and show that cleavage rate drives sequence conservation in the glmS ribozyme.

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Autores principales:	Johan O. L. Andreasson, Andrew Savinov, Steven M. Block, William J. Greenleaf
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/bd2de6930e9143e08b42ce2dd005876a
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spelling	oai:doaj.org-article:bd2de6930e9143e08b42ce2dd005876a2021-12-02T16:56:31ZComprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme10.1038/s41467-020-15540-12041-1723https://doaj.org/article/bd2de6930e9143e08b42ce2dd005876a2020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15540-1https://doaj.org/toc/2041-1723RNA sequence-structure-function relationships can be studied via assays of variant activity. The authors developed a method to systematically determine ribozyme variant kinetics, using it to map double-mutant interactions and show that cleavage rate drives sequence conservation in the glmS ribozyme.Johan O. L. AndreassonAndrew SavinovSteven M. BlockWilliam J. GreenleafNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Johan O. L. Andreasson Andrew Savinov Steven M. Block William J. Greenleaf Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme
description	RNA sequence-structure-function relationships can be studied via assays of variant activity. The authors developed a method to systematically determine ribozyme variant kinetics, using it to map double-mutant interactions and show that cleavage rate drives sequence conservation in the glmS ribozyme.
format	article
author	Johan O. L. Andreasson Andrew Savinov Steven M. Block William J. Greenleaf
author_facet	Johan O. L. Andreasson Andrew Savinov Steven M. Block William J. Greenleaf
author_sort	Johan O. L. Andreasson
title	Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme
title_short	Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme
title_full	Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme
title_fullStr	Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme
title_full_unstemmed	Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme
title_sort	comprehensive sequence-to-function mapping of cofactor-dependent rna catalysis in the glms ribozyme
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/bd2de6930e9143e08b42ce2dd005876a
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Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme

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