Live-cell protein labelling with nanometre precision by cell squeezing

The direct observation of intracellular processes through microscopy can provide key insight into biological pathways. Here the authors demonstrate the combination of microfluidic cell squeezing and fluorescent label delivery to native proteins for high throughput live cell super-resolution imaging.

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Detalles Bibliográficos
Autores principales: Alina Kollmannsperger, Armon Sharei, Anika Raulf, Mike Heilemann, Robert Langer, Klavs F. Jensen, Ralph Wieneke, Robert Tampé
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/bd3921c263ee4105b737de9243e26428
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Sumario:The direct observation of intracellular processes through microscopy can provide key insight into biological pathways. Here the authors demonstrate the combination of microfluidic cell squeezing and fluorescent label delivery to native proteins for high throughput live cell super-resolution imaging.