Live-cell protein labelling with nanometre precision by cell squeezing

The direct observation of intracellular processes through microscopy can provide key insight into biological pathways. Here the authors demonstrate the combination of microfluidic cell squeezing and fluorescent label delivery to native proteins for high throughput live cell super-resolution imaging.

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Autores principales:	Alina Kollmannsperger, Armon Sharei, Anika Raulf, Mike Heilemann, Robert Langer, Klavs F. Jensen, Ralph Wieneke, Robert Tampé
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/bd3921c263ee4105b737de9243e26428
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spelling	oai:doaj.org-article:bd3921c263ee4105b737de9243e264282021-12-02T15:35:46ZLive-cell protein labelling with nanometre precision by cell squeezing10.1038/ncomms103722041-1723https://doaj.org/article/bd3921c263ee4105b737de9243e264282016-01-01T00:00:00Zhttps://doi.org/10.1038/ncomms10372https://doaj.org/toc/2041-1723The direct observation of intracellular processes through microscopy can provide key insight into biological pathways. Here the authors demonstrate the combination of microfluidic cell squeezing and fluorescent label delivery to native proteins for high throughput live cell super-resolution imaging.Alina KollmannspergerArmon ShareiAnika RaulfMike HeilemannRobert LangerKlavs F. JensenRalph WienekeRobert TampéNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-7 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Alina Kollmannsperger Armon Sharei Anika Raulf Mike Heilemann Robert Langer Klavs F. Jensen Ralph Wieneke Robert Tampé Live-cell protein labelling with nanometre precision by cell squeezing
description	The direct observation of intracellular processes through microscopy can provide key insight into biological pathways. Here the authors demonstrate the combination of microfluidic cell squeezing and fluorescent label delivery to native proteins for high throughput live cell super-resolution imaging.
format	article
author	Alina Kollmannsperger Armon Sharei Anika Raulf Mike Heilemann Robert Langer Klavs F. Jensen Ralph Wieneke Robert Tampé
author_facet	Alina Kollmannsperger Armon Sharei Anika Raulf Mike Heilemann Robert Langer Klavs F. Jensen Ralph Wieneke Robert Tampé
author_sort	Alina Kollmannsperger
title	Live-cell protein labelling with nanometre precision by cell squeezing
title_short	Live-cell protein labelling with nanometre precision by cell squeezing
title_full	Live-cell protein labelling with nanometre precision by cell squeezing
title_fullStr	Live-cell protein labelling with nanometre precision by cell squeezing
title_full_unstemmed	Live-cell protein labelling with nanometre precision by cell squeezing
title_sort	live-cell protein labelling with nanometre precision by cell squeezing
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/bd3921c263ee4105b737de9243e26428
work_keys_str_mv	AT alinakollmannsperger livecellproteinlabellingwithnanometreprecisionbycellsqueezing AT armonsharei livecellproteinlabellingwithnanometreprecisionbycellsqueezing AT anikaraulf livecellproteinlabellingwithnanometreprecisionbycellsqueezing AT mikeheilemann livecellproteinlabellingwithnanometreprecisionbycellsqueezing AT robertlanger livecellproteinlabellingwithnanometreprecisionbycellsqueezing AT klavsfjensen livecellproteinlabellingwithnanometreprecisionbycellsqueezing AT ralphwieneke livecellproteinlabellingwithnanometreprecisionbycellsqueezing AT roberttampe livecellproteinlabellingwithnanometreprecisionbycellsqueezing
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Live-cell protein labelling with nanometre precision by cell squeezing

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