Development and evaluation of the magnetic particle-based chemiluminescence immunoassay for rapid and quantitative detection of Aflatoxin B1 in foodstuff

Herein, a simple and cost-effective chemiluminescence immunoassay combined with the magnetic particles (MPCLIA) is presented for the rapid detection and analysis of Aflatoxin B1, which is a potent carcinogen found in the environment. Several physicochemical factors that potentially influence the ass...

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Autores principales:	Gang Xie, Ming Zhu, Zhenjiang Liu, Bo Zhang, Mingjin Shi, Shuo Wang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	chemiluminescence immunoassay magnetic particles aflatoxin b1 real samples Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/bd3e091f54c14429bd81b8fbb29c10b6
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spelling	oai:doaj.org-article:bd3e091f54c14429bd81b8fbb29c10b62021-11-26T11:19:46ZDevelopment and evaluation of the magnetic particle-based chemiluminescence immunoassay for rapid and quantitative detection of Aflatoxin B1 in foodstuff0954-01051465-344310.1080/09540105.2017.1416591https://doaj.org/article/bd3e091f54c14429bd81b8fbb29c10b62018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1416591https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Herein, a simple and cost-effective chemiluminescence immunoassay combined with the magnetic particles (MPCLIA) is presented for the rapid detection and analysis of Aflatoxin B1, which is a potent carcinogen found in the environment. Several physicochemical factors that potentially influence the assay performance of the proposed method were investigated in detail, including concentrations of FITC-labelled antibody, AFB1-alkaline phosphatase and magnetic particles concentration. Under the optimized experiment condition, the method showed: (i) a low limit detection of 0.05 ng/mL; (ii) satisfactory recoveries in real grain and oil samples ranging from 78% to 109%, which was well within the requirement of residue analysis, and (iii) excellent sensitivity, wide dynamic range and easy to separate in the washing process, which was superior to enzyme-linked immunosorbent assay (ELISA) method. In addition, the device used is of low cost. Moreover, the MPCLIA results for the real samples were in good agreement with that obtained by the high-performance liquid chromatography. These results suggest that the proposed MPCLIA method has potential application for screening other toxic mycotoxins occur in the food industry or other applied fields.Gang XieMing ZhuZhenjiang LiuBo ZhangMingjin ShiShuo WangTaylor & Francis Grouparticlechemiluminescence immunoassaymagnetic particlesaflatoxin b1real samplesAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 564-576 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	chemiluminescence immunoassay magnetic particles aflatoxin b1 real samples Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	chemiluminescence immunoassay magnetic particles aflatoxin b1 real samples Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Gang Xie Ming Zhu Zhenjiang Liu Bo Zhang Mingjin Shi Shuo Wang Development and evaluation of the magnetic particle-based chemiluminescence immunoassay for rapid and quantitative detection of Aflatoxin B1 in foodstuff
description	Herein, a simple and cost-effective chemiluminescence immunoassay combined with the magnetic particles (MPCLIA) is presented for the rapid detection and analysis of Aflatoxin B1, which is a potent carcinogen found in the environment. Several physicochemical factors that potentially influence the assay performance of the proposed method were investigated in detail, including concentrations of FITC-labelled antibody, AFB1-alkaline phosphatase and magnetic particles concentration. Under the optimized experiment condition, the method showed: (i) a low limit detection of 0.05 ng/mL; (ii) satisfactory recoveries in real grain and oil samples ranging from 78% to 109%, which was well within the requirement of residue analysis, and (iii) excellent sensitivity, wide dynamic range and easy to separate in the washing process, which was superior to enzyme-linked immunosorbent assay (ELISA) method. In addition, the device used is of low cost. Moreover, the MPCLIA results for the real samples were in good agreement with that obtained by the high-performance liquid chromatography. These results suggest that the proposed MPCLIA method has potential application for screening other toxic mycotoxins occur in the food industry or other applied fields.
format	article
author	Gang Xie Ming Zhu Zhenjiang Liu Bo Zhang Mingjin Shi Shuo Wang
author_facet	Gang Xie Ming Zhu Zhenjiang Liu Bo Zhang Mingjin Shi Shuo Wang
author_sort	Gang Xie
title	Development and evaluation of the magnetic particle-based chemiluminescence immunoassay for rapid and quantitative detection of Aflatoxin B1 in foodstuff
title_short	Development and evaluation of the magnetic particle-based chemiluminescence immunoassay for rapid and quantitative detection of Aflatoxin B1 in foodstuff
title_full	Development and evaluation of the magnetic particle-based chemiluminescence immunoassay for rapid and quantitative detection of Aflatoxin B1 in foodstuff
title_fullStr	Development and evaluation of the magnetic particle-based chemiluminescence immunoassay for rapid and quantitative detection of Aflatoxin B1 in foodstuff
title_full_unstemmed	Development and evaluation of the magnetic particle-based chemiluminescence immunoassay for rapid and quantitative detection of Aflatoxin B1 in foodstuff
title_sort	development and evaluation of the magnetic particle-based chemiluminescence immunoassay for rapid and quantitative detection of aflatoxin b1 in foodstuff
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/bd3e091f54c14429bd81b8fbb29c10b6
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Development and evaluation of the magnetic particle-based chemiluminescence immunoassay for rapid and quantitative detection of Aflatoxin B1 in foodstuff

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