In vitro biocompatibility of calcined mesoporous silica particles and fetal blood cells

Mohammed T Al Samri,1,* Ankush V Biradar,2,3,* Ahmed R Alsuwaidi,1 Ghazala Balhaj,1 Suleiman Al-Hammadi,1 Safa Shehab,4 Suhail Al-Salam,5 Saeed Tariq,4 Thachillath Pramathan,1 Sheela Benedict,1 Tewodros Asefa,2,3 Abdul-Kader Souid11Department of Pediatrics, Abu Dhabi, United Arab Emirates; 2Departme...

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Autores principales:	Al Samri MT, Biradar AV, Alsuwaidi AR, Balhaj G, Al-Hammadi S, Shehab S, Al-Salam S, Tariq S, Pramathan T, Benedict S, Asefa T, Souid AK
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bd47137c13634146a635489051bc25d2
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spelling	oai:doaj.org-article:bd47137c13634146a635489051bc25d22021-12-02T01:11:24ZIn vitro biocompatibility of calcined mesoporous silica particles and fetal blood cells1176-91141178-2013https://doaj.org/article/bd47137c13634146a635489051bc25d22012-08-01T00:00:00Zhttp://www.dovepress.com/in-vitro-biocompatibility-of-calcined-mesoporous-silica-particles-and--a10610https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Mohammed T Al Samri,1,* Ankush V Biradar,2,3,* Ahmed R Alsuwaidi,1 Ghazala Balhaj,1 Suleiman Al-Hammadi,1 Safa Shehab,4 Suhail Al-Salam,5 Saeed Tariq,4 Thachillath Pramathan,1 Sheela Benedict,1 Tewodros Asefa,2,3 Abdul-Kader Souid11Department of Pediatrics, Abu Dhabi, United Arab Emirates; 2Department of Chemistry and Chemical Biology, 3Department of Chemical Engineering and Biochemical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ, USA; 4Department of Anatomy, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain, Abu Dhabi, United Arab Emirates; 5Department of Pathology Faculty of Medicine and Health Sciences, United Arab Emirates University, Al ain, Abu Dhabi, United Arab Emirates*These authors contributed equally to this workBackground: The biocompatibility of two forms of calcined mesoporous silica particles, labeled as MCM41-cal and SBA15-cal, with fetal blood mononuclear cells was assessed in vitro.Methods and results: Fetal mononuclear cells were isolated from umbilical cord blood and exposed to 0.5 mg/mL of MCM41-cal or SBA15-cal for several hours. Transmission electron micrographs confirmed the presence of particles in the cytosol of macrophages, neutrophils, and lymphocytes without noticeable damage to the cellular organelles. The particles (especially MCM41-cal) were in close proximity to plasma, and nuclear and mitochondrial membranes. Biocompatibility was assessed by a functional assay that measured cellular respiration, ie, mitochondrial O2 consumption. The rate of respiration (kc, in &micro;M O2 per minute per 107 cells) for untreated cells was 0.42 &plusmn; 0.16 (n = 10), for cells treated with MCM41-cal was 0.39 &plusmn; 0.22 (n = 5, P &gt; 0.966) and for cells treated with SBA15-cal was 0.44 &plusmn; 0.13 (n = 5, P &gt;0.981).Conclusion: The results show reasonable biocompatibility of MCM41-cal and SBA15-cal in fetal blood mononuclear cells. Future studies are needed to determine the potential of collecting fetal cells from a fetus or neonate, loading the cells in vitro with therapeutic MCM41-cal or SBA15-cal, and reinfusing them into the fetus or neonate.Keywords: mesoporous silica, nanomaterials, biocompatibility, bioenergetics, in vitro, fetal cellsAl Samri MTBiradar AVAlsuwaidi ARBalhaj GAl-Hammadi SShehab SAl-Salam STariq SPramathan TBenedict SAsefa TSouid AKDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3111-3121 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Al Samri MT Biradar AV Alsuwaidi AR Balhaj G Al-Hammadi S Shehab S Al-Salam S Tariq S Pramathan T Benedict S Asefa T Souid AK In vitro biocompatibility of calcined mesoporous silica particles and fetal blood cells
description	Mohammed T Al Samri,1,* Ankush V Biradar,2,3,* Ahmed R Alsuwaidi,1 Ghazala Balhaj,1 Suleiman Al-Hammadi,1 Safa Shehab,4 Suhail Al-Salam,5 Saeed Tariq,4 Thachillath Pramathan,1 Sheela Benedict,1 Tewodros Asefa,2,3 Abdul-Kader Souid11Department of Pediatrics, Abu Dhabi, United Arab Emirates; 2Department of Chemistry and Chemical Biology, 3Department of Chemical Engineering and Biochemical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ, USA; 4Department of Anatomy, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain, Abu Dhabi, United Arab Emirates; 5Department of Pathology Faculty of Medicine and Health Sciences, United Arab Emirates University, Al ain, Abu Dhabi, United Arab Emirates*These authors contributed equally to this workBackground: The biocompatibility of two forms of calcined mesoporous silica particles, labeled as MCM41-cal and SBA15-cal, with fetal blood mononuclear cells was assessed in vitro.Methods and results: Fetal mononuclear cells were isolated from umbilical cord blood and exposed to 0.5 mg/mL of MCM41-cal or SBA15-cal for several hours. Transmission electron micrographs confirmed the presence of particles in the cytosol of macrophages, neutrophils, and lymphocytes without noticeable damage to the cellular organelles. The particles (especially MCM41-cal) were in close proximity to plasma, and nuclear and mitochondrial membranes. Biocompatibility was assessed by a functional assay that measured cellular respiration, ie, mitochondrial O2 consumption. The rate of respiration (kc, in &micro;M O2 per minute per 107 cells) for untreated cells was 0.42 &plusmn; 0.16 (n = 10), for cells treated with MCM41-cal was 0.39 &plusmn; 0.22 (n = 5, P &gt; 0.966) and for cells treated with SBA15-cal was 0.44 &plusmn; 0.13 (n = 5, P &gt;0.981).Conclusion: The results show reasonable biocompatibility of MCM41-cal and SBA15-cal in fetal blood mononuclear cells. Future studies are needed to determine the potential of collecting fetal cells from a fetus or neonate, loading the cells in vitro with therapeutic MCM41-cal or SBA15-cal, and reinfusing them into the fetus or neonate.Keywords: mesoporous silica, nanomaterials, biocompatibility, bioenergetics, in vitro, fetal cells
format	article
author	Al Samri MT Biradar AV Alsuwaidi AR Balhaj G Al-Hammadi S Shehab S Al-Salam S Tariq S Pramathan T Benedict S Asefa T Souid AK
author_facet	Al Samri MT Biradar AV Alsuwaidi AR Balhaj G Al-Hammadi S Shehab S Al-Salam S Tariq S Pramathan T Benedict S Asefa T Souid AK
author_sort	Al Samri MT
title	In vitro biocompatibility of calcined mesoporous silica particles and fetal blood cells
title_short	In vitro biocompatibility of calcined mesoporous silica particles and fetal blood cells
title_full	In vitro biocompatibility of calcined mesoporous silica particles and fetal blood cells
title_fullStr	In vitro biocompatibility of calcined mesoporous silica particles and fetal blood cells
title_full_unstemmed	In vitro biocompatibility of calcined mesoporous silica particles and fetal blood cells
title_sort	in vitro biocompatibility of calcined mesoporous silica particles and fetal blood cells
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/bd47137c13634146a635489051bc25d2
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In vitro biocompatibility of calcined mesoporous silica particles and fetal blood cells

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