Biogeography of the Respiratory Tract Microbiome in Patients With Malignant Tracheal Tumors

BackgroundThis study aimed to characterize the bacterial microbiota in the oral cavity (OC), throat, trachea, and distal alveoli of patients with primary malignant tracheal tumors (PMTT), including squamous cell carcinoma (SCC) and salivary gland carcinoma patients (SGC), for comparison with a match...

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Autores principales:	Kai-Xiong Liu, Hai-Xia Liu, Jing Zhang, Nan Zhang, Yun-Zhi Zhou, Mei-Mei Tao, Hong-Wu Wang, Jie-Ming Qu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	malignant tracheal tumor squamous cell carcinoma salivary gland type carcinoma 16S rRNA sequencing microbiome Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/bd49f38c20a4493cb16de768fb947c21
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spelling	oai:doaj.org-article:bd49f38c20a4493cb16de768fb947c212021-11-19T06:23:59ZBiogeography of the Respiratory Tract Microbiome in Patients With Malignant Tracheal Tumors2234-943X10.3389/fonc.2021.758917https://doaj.org/article/bd49f38c20a4493cb16de768fb947c212021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fonc.2021.758917/fullhttps://doaj.org/toc/2234-943XBackgroundThis study aimed to characterize the bacterial microbiota in the oral cavity (OC), throat, trachea, and distal alveoli of patients with primary malignant tracheal tumors (PMTT), including squamous cell carcinoma (SCC) and salivary gland carcinoma patients (SGC), for comparison with a matched non-malignant tracheal tumor (NMTT) group.MethodsPatients with pathological diagnosis of PMTT and NMTT were included in this study. Saliva, throat swab (TS), trachea protected specimen brush (PSB), and bronchoalveolar lavage fluid (BALF) samples were collected for 16S rRNA gene sequencing. The composition, diversity, and distribution of the microbiota were compared among biogeographic sampling sites and patient groups. The relationship between the genera-level taxon abundance and tracheal tumor types was also investigated to screen for candidate biomarkers.FindingsThe most represented phyla in the four sites were Bacteroidetes, Firmicutes, Proteobacteria, and Fusobacteria. In SCC patients, the relative abundance of Bacteroidetes and Firmicutes gradually decreased with increasing depth into the respiratory tract, while the relative abundance of Proteobacteria gradually increased. Bacterial communities at the four biogeographic sites formed two distinct clusters, with OC and TS samples comprising one cluster and PSB and BALF samples comprising the other group. Principal coordinate analysis showed that trachea microbiota in SCC patients were distinct from that of SGC or NMTT patients. In the trachea, AUCs generated by Prevotella and Alloprevotella showed that the abundance of these genera could distinguish SCC patients from both NMTT and SGC patients.InterpretationThe structure of respiratory tract microbiota in PMTT patients is related to tumor type. Certain bacteria could potentially serve as markers of SCC, although verification with large-sample studies is necessary.Kai-Xiong LiuKai-Xiong LiuKai-Xiong LiuKai-Xiong LiuHai-Xia LiuJing ZhangNan ZhangYun-Zhi ZhouMei-Mei TaoHong-Wu WangJie-Ming QuJie-Ming QuJie-Ming QuFrontiers Media S.A.articlemalignant tracheal tumorsquamous cell carcinomasalivary gland type carcinoma16S rRNA sequencingmicrobiomeNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENFrontiers in Oncology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	malignant tracheal tumor squamous cell carcinoma salivary gland type carcinoma 16S rRNA sequencing microbiome Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	malignant tracheal tumor squamous cell carcinoma salivary gland type carcinoma 16S rRNA sequencing microbiome Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Kai-Xiong Liu Kai-Xiong Liu Kai-Xiong Liu Kai-Xiong Liu Hai-Xia Liu Jing Zhang Nan Zhang Yun-Zhi Zhou Mei-Mei Tao Hong-Wu Wang Jie-Ming Qu Jie-Ming Qu Jie-Ming Qu Biogeography of the Respiratory Tract Microbiome in Patients With Malignant Tracheal Tumors
description	BackgroundThis study aimed to characterize the bacterial microbiota in the oral cavity (OC), throat, trachea, and distal alveoli of patients with primary malignant tracheal tumors (PMTT), including squamous cell carcinoma (SCC) and salivary gland carcinoma patients (SGC), for comparison with a matched non-malignant tracheal tumor (NMTT) group.MethodsPatients with pathological diagnosis of PMTT and NMTT were included in this study. Saliva, throat swab (TS), trachea protected specimen brush (PSB), and bronchoalveolar lavage fluid (BALF) samples were collected for 16S rRNA gene sequencing. The composition, diversity, and distribution of the microbiota were compared among biogeographic sampling sites and patient groups. The relationship between the genera-level taxon abundance and tracheal tumor types was also investigated to screen for candidate biomarkers.FindingsThe most represented phyla in the four sites were Bacteroidetes, Firmicutes, Proteobacteria, and Fusobacteria. In SCC patients, the relative abundance of Bacteroidetes and Firmicutes gradually decreased with increasing depth into the respiratory tract, while the relative abundance of Proteobacteria gradually increased. Bacterial communities at the four biogeographic sites formed two distinct clusters, with OC and TS samples comprising one cluster and PSB and BALF samples comprising the other group. Principal coordinate analysis showed that trachea microbiota in SCC patients were distinct from that of SGC or NMTT patients. In the trachea, AUCs generated by Prevotella and Alloprevotella showed that the abundance of these genera could distinguish SCC patients from both NMTT and SGC patients.InterpretationThe structure of respiratory tract microbiota in PMTT patients is related to tumor type. Certain bacteria could potentially serve as markers of SCC, although verification with large-sample studies is necessary.
format	article
author	Kai-Xiong Liu Kai-Xiong Liu Kai-Xiong Liu Kai-Xiong Liu Hai-Xia Liu Jing Zhang Nan Zhang Yun-Zhi Zhou Mei-Mei Tao Hong-Wu Wang Jie-Ming Qu Jie-Ming Qu Jie-Ming Qu
author_facet	Kai-Xiong Liu Kai-Xiong Liu Kai-Xiong Liu Kai-Xiong Liu Hai-Xia Liu Jing Zhang Nan Zhang Yun-Zhi Zhou Mei-Mei Tao Hong-Wu Wang Jie-Ming Qu Jie-Ming Qu Jie-Ming Qu
author_sort	Kai-Xiong Liu
title	Biogeography of the Respiratory Tract Microbiome in Patients With Malignant Tracheal Tumors
title_short	Biogeography of the Respiratory Tract Microbiome in Patients With Malignant Tracheal Tumors
title_full	Biogeography of the Respiratory Tract Microbiome in Patients With Malignant Tracheal Tumors
title_fullStr	Biogeography of the Respiratory Tract Microbiome in Patients With Malignant Tracheal Tumors
title_full_unstemmed	Biogeography of the Respiratory Tract Microbiome in Patients With Malignant Tracheal Tumors
title_sort	biogeography of the respiratory tract microbiome in patients with malignant tracheal tumors
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/bd49f38c20a4493cb16de768fb947c21
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Biogeography of the Respiratory Tract Microbiome in Patients With Malignant Tracheal Tumors

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