Varietal Discrimination of Pineapple (<i>Ananas comosus</i> L.) Using Chromatographic Fingerprints and Chemometrics

Online solid-phase extraction-liquid chromatography (online SPE-LC) with diode array detector (DAD) was used to obtain the chromatographic fingerprint of bioactive compounds of pineapple (Ananas comosus L.). The extracts from 40 samples of three different varieties of pineapple (Morris, MD2, and Jos...

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Autores principales: Almie Amira Munaras Khan, Norashikin Saim, Rossuriati Dol Hamid, Rozita Osman, Siti Raihan Zakaria
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Lenguaje:EN
Publicado: Department of Chemistry, Universitas Gadjah Mada 2020
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Acceso en línea:https://doaj.org/article/bd4ddef4106f4372a6b192e982809d2a
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spelling oai:doaj.org-article:bd4ddef4106f4372a6b192e982809d2a2021-12-02T11:27:28ZVarietal Discrimination of Pineapple (<i>Ananas comosus</i> L.) Using Chromatographic Fingerprints and Chemometrics1411-94202460-157810.22146/ijc.47159https://doaj.org/article/bd4ddef4106f4372a6b192e982809d2a2020-07-01T00:00:00Zhttps://jurnal.ugm.ac.id/ijc/article/view/47159https://doaj.org/toc/1411-9420https://doaj.org/toc/2460-1578Online solid-phase extraction-liquid chromatography (online SPE-LC) with diode array detector (DAD) was used to obtain the chromatographic fingerprint of bioactive compounds of pineapple (Ananas comosus L.). The extracts from 40 samples of three different varieties of pineapple (Morris, MD2, and Josaphine) were obtained using pressurized liquid extraction (PLE) prior to separation using online SPE-LC. The SPE-LC method was optimized and validated and applied to 40 pineapple samples of those three varieties. Seven bioactive compounds identified include catechin, epicatechin, chlorogenic acid, ferulic acid, quercetin, myricetin, and bromelain. For varietal discrimination, the relative areas of 16 selected peaks were subjected to chemometric techniques. The three pineapple varieties were successfully discriminated using cluster analysis (CA) and principal component analysis (PCA).Almie Amira Munaras KhanNorashikin SaimRossuriati Dol HamidRozita OsmanSiti Raihan ZakariaDepartment of Chemistry, Universitas Gadjah Madaarticleonline spe-lcpineapplebioactive compoundschromatographic fingerprintchemometricsChemistryQD1-999ENIndonesian Journal of Chemistry, Vol 20, Iss 5, Pp 1052-1060 (2020)
institution DOAJ
collection DOAJ
language EN
topic online spe-lc
pineapple
bioactive compounds
chromatographic fingerprint
chemometrics
Chemistry
QD1-999
spellingShingle online spe-lc
pineapple
bioactive compounds
chromatographic fingerprint
chemometrics
Chemistry
QD1-999
Almie Amira Munaras Khan
Norashikin Saim
Rossuriati Dol Hamid
Rozita Osman
Siti Raihan Zakaria
Varietal Discrimination of Pineapple (<i>Ananas comosus</i> L.) Using Chromatographic Fingerprints and Chemometrics
description Online solid-phase extraction-liquid chromatography (online SPE-LC) with diode array detector (DAD) was used to obtain the chromatographic fingerprint of bioactive compounds of pineapple (Ananas comosus L.). The extracts from 40 samples of three different varieties of pineapple (Morris, MD2, and Josaphine) were obtained using pressurized liquid extraction (PLE) prior to separation using online SPE-LC. The SPE-LC method was optimized and validated and applied to 40 pineapple samples of those three varieties. Seven bioactive compounds identified include catechin, epicatechin, chlorogenic acid, ferulic acid, quercetin, myricetin, and bromelain. For varietal discrimination, the relative areas of 16 selected peaks were subjected to chemometric techniques. The three pineapple varieties were successfully discriminated using cluster analysis (CA) and principal component analysis (PCA).
format article
author Almie Amira Munaras Khan
Norashikin Saim
Rossuriati Dol Hamid
Rozita Osman
Siti Raihan Zakaria
author_facet Almie Amira Munaras Khan
Norashikin Saim
Rossuriati Dol Hamid
Rozita Osman
Siti Raihan Zakaria
author_sort Almie Amira Munaras Khan
title Varietal Discrimination of Pineapple (<i>Ananas comosus</i> L.) Using Chromatographic Fingerprints and Chemometrics
title_short Varietal Discrimination of Pineapple (<i>Ananas comosus</i> L.) Using Chromatographic Fingerprints and Chemometrics
title_full Varietal Discrimination of Pineapple (<i>Ananas comosus</i> L.) Using Chromatographic Fingerprints and Chemometrics
title_fullStr Varietal Discrimination of Pineapple (<i>Ananas comosus</i> L.) Using Chromatographic Fingerprints and Chemometrics
title_full_unstemmed Varietal Discrimination of Pineapple (<i>Ananas comosus</i> L.) Using Chromatographic Fingerprints and Chemometrics
title_sort varietal discrimination of pineapple (<i>ananas comosus</i> l.) using chromatographic fingerprints and chemometrics
publisher Department of Chemistry, Universitas Gadjah Mada
publishDate 2020
url https://doaj.org/article/bd4ddef4106f4372a6b192e982809d2a
work_keys_str_mv AT almieamiramunaraskhan varietaldiscriminationofpineappleiananascomosusilusingchromatographicfingerprintsandchemometrics
AT norashikinsaim varietaldiscriminationofpineappleiananascomosusilusingchromatographicfingerprintsandchemometrics
AT rossuriatidolhamid varietaldiscriminationofpineappleiananascomosusilusingchromatographicfingerprintsandchemometrics
AT rozitaosman varietaldiscriminationofpineappleiananascomosusilusingchromatographicfingerprintsandchemometrics
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