Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia

Abstract. Saputra AW, Yuda P. 2020. Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia. Biodiversitas 21: 4772-4777. Maleo Senkawor (Macrocephalon maleo), an endemic and endangered bird of Sulawesi (Indonesia)...

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Autores principales:	Andie Wijaya Saputra, Pramana Yuda
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	differentiation, genetic diversity, maleo, mtdna control region Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bd4fa05a59f34c8d833fa48daad7e425
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spelling	oai:doaj.org-article:bd4fa05a59f34c8d833fa48daad7e4252021-11-22T00:51:08ZLow genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia1412-033X2085-472210.13057/biodiv/d211042https://doaj.org/article/bd4fa05a59f34c8d833fa48daad7e4252020-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6188https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Saputra AW, Yuda P. 2020. Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia. Biodiversitas 21: 4772-4777. Maleo Senkawor (Macrocephalon maleo), an endemic and endangered bird of Sulawesi (Indonesia), is burrow-nesting megapodes that incubate its eggs in communal nesting sites in soils heated by sun on beaches and by volcanic activity in inland. The aims of this study were to assess genetic diversity of the Maleo and examine whether those which have different nesting sites have become genetically differentiated. In total, 24 eggshell membranes of Maleo were collected from Tanjung Binerean (coastal nesting ground) and Tambun (inland nesting ground), and the DNA was extracted using silica spin-column kit. PCR was applied to amplify the hypervariable region 1 (HV1) and partial mtDNA control region of HV2 using a specific primer set designed for Maleo. The PCR products were sequenced, resulted in 612 bp, and showed 9 polymorphic sites and 9 haplotypes (H). Further sequences analysis suggested that there was no genetic differentiation between coastal nesting population and inland nesting population (Fst = 0.0009; P = 0.431). As expected, the genetic diversity of Maleo was relatively low (coastal nesting population, Hd: 0.727270, ?: 0.002377 and inland nesting population Hd: 0.848480, ?: 0.002203).Andie Wijaya SaputraPramana YudaMBI & UNS Soloarticledifferentiation, genetic diversity, maleo, mtdna control regionBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	differentiation, genetic diversity, maleo, mtdna control region Biology (General) QH301-705.5
spellingShingle	differentiation, genetic diversity, maleo, mtdna control region Biology (General) QH301-705.5 Andie Wijaya Saputra Pramana Yuda Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia
description	Abstract. Saputra AW, Yuda P. 2020. Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia. Biodiversitas 21: 4772-4777. Maleo Senkawor (Macrocephalon maleo), an endemic and endangered bird of Sulawesi (Indonesia), is burrow-nesting megapodes that incubate its eggs in communal nesting sites in soils heated by sun on beaches and by volcanic activity in inland. The aims of this study were to assess genetic diversity of the Maleo and examine whether those which have different nesting sites have become genetically differentiated. In total, 24 eggshell membranes of Maleo were collected from Tanjung Binerean (coastal nesting ground) and Tambun (inland nesting ground), and the DNA was extracted using silica spin-column kit. PCR was applied to amplify the hypervariable region 1 (HV1) and partial mtDNA control region of HV2 using a specific primer set designed for Maleo. The PCR products were sequenced, resulted in 612 bp, and showed 9 polymorphic sites and 9 haplotypes (H). Further sequences analysis suggested that there was no genetic differentiation between coastal nesting population and inland nesting population (Fst = 0.0009; P = 0.431). As expected, the genetic diversity of Maleo was relatively low (coastal nesting population, Hd: 0.727270, ?: 0.002377 and inland nesting population Hd: 0.848480, ?: 0.002203).
format	article
author	Andie Wijaya Saputra Pramana Yuda
author_facet	Andie Wijaya Saputra Pramana Yuda
author_sort	Andie Wijaya Saputra
title	Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia
title_short	Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia
title_full	Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia
title_fullStr	Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia
title_full_unstemmed	Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia
title_sort	low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in north sulawesi, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/bd4fa05a59f34c8d833fa48daad7e425
work_keys_str_mv	AT andiewijayasaputra lowgeneticdiversityandnogeneticdifferentiationbetweenmaleohatchedatcoastalandinlandnestinggroundsinnorthsulawesiindonesia AT pramanayuda lowgeneticdiversityandnogeneticdifferentiationbetweenmaleohatchedatcoastalandinlandnestinggroundsinnorthsulawesiindonesia
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Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia

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