Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia

Abstract. Saputra AW, Yuda P. 2020. Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia. Biodiversitas 21: 4772-4777. Maleo Senkawor (Macrocephalon maleo), an endemic and endangered bird of Sulawesi (Indonesia)...

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Autores principales: Andie Wijaya Saputra, Pramana Yuda
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Publicado: MBI & UNS Solo 2020
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spelling oai:doaj.org-article:bd4fa05a59f34c8d833fa48daad7e4252021-11-22T00:51:08ZLow genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia1412-033X2085-472210.13057/biodiv/d211042https://doaj.org/article/bd4fa05a59f34c8d833fa48daad7e4252020-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6188https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Saputra AW, Yuda P. 2020. Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia. Biodiversitas 21: 4772-4777. Maleo Senkawor (Macrocephalon maleo), an endemic and endangered bird of Sulawesi (Indonesia), is burrow-nesting megapodes that incubate its eggs in communal nesting sites in soils heated by sun on beaches and by volcanic activity in inland. The aims of this study were to assess genetic diversity of the Maleo and examine whether those which have different nesting sites have become genetically differentiated. In total, 24 eggshell membranes of Maleo were collected from Tanjung Binerean (coastal nesting ground) and Tambun (inland nesting ground), and the DNA was extracted using silica spin-column kit. PCR was applied to amplify the hypervariable region 1 (HV1) and partial mtDNA control region of HV2 using a specific primer set designed for Maleo. The PCR products were sequenced, resulted in 612 bp, and showed 9 polymorphic sites and 9 haplotypes (H). Further sequences analysis suggested that there was no genetic differentiation between coastal nesting population and inland nesting population (Fst = 0.0009; P = 0.431). As expected, the genetic diversity of Maleo was relatively low (coastal nesting population, Hd: 0.727270, ?: 0.002377 and inland nesting population Hd: 0.848480, ?: 0.002203).Andie Wijaya SaputraPramana YudaMBI & UNS Soloarticledifferentiation, genetic diversity, maleo, mtdna control regionBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 10 (2020)
institution DOAJ
collection DOAJ
language EN
topic differentiation, genetic diversity, maleo, mtdna control region
Biology (General)
QH301-705.5
spellingShingle differentiation, genetic diversity, maleo, mtdna control region
Biology (General)
QH301-705.5
Andie Wijaya Saputra
Pramana Yuda
Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia
description Abstract. Saputra AW, Yuda P. 2020. Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia. Biodiversitas 21: 4772-4777. Maleo Senkawor (Macrocephalon maleo), an endemic and endangered bird of Sulawesi (Indonesia), is burrow-nesting megapodes that incubate its eggs in communal nesting sites in soils heated by sun on beaches and by volcanic activity in inland. The aims of this study were to assess genetic diversity of the Maleo and examine whether those which have different nesting sites have become genetically differentiated. In total, 24 eggshell membranes of Maleo were collected from Tanjung Binerean (coastal nesting ground) and Tambun (inland nesting ground), and the DNA was extracted using silica spin-column kit. PCR was applied to amplify the hypervariable region 1 (HV1) and partial mtDNA control region of HV2 using a specific primer set designed for Maleo. The PCR products were sequenced, resulted in 612 bp, and showed 9 polymorphic sites and 9 haplotypes (H). Further sequences analysis suggested that there was no genetic differentiation between coastal nesting population and inland nesting population (Fst = 0.0009; P = 0.431). As expected, the genetic diversity of Maleo was relatively low (coastal nesting population, Hd: 0.727270, ?: 0.002377 and inland nesting population Hd: 0.848480, ?: 0.002203).
format article
author Andie Wijaya Saputra
Pramana Yuda
author_facet Andie Wijaya Saputra
Pramana Yuda
author_sort Andie Wijaya Saputra
title Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia
title_short Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia
title_full Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia
title_fullStr Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia
title_full_unstemmed Low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in North Sulawesi, Indonesia
title_sort low genetic diversity and no genetic differentiation between maleo hatched at coastal and inland nesting grounds in north sulawesi, indonesia
publisher MBI & UNS Solo
publishDate 2020
url https://doaj.org/article/bd4fa05a59f34c8d833fa48daad7e425
work_keys_str_mv AT andiewijayasaputra lowgeneticdiversityandnogeneticdifferentiationbetweenmaleohatchedatcoastalandinlandnestinggroundsinnorthsulawesiindonesia
AT pramanayuda lowgeneticdiversityandnogeneticdifferentiationbetweenmaleohatchedatcoastalandinlandnestinggroundsinnorthsulawesiindonesia
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