Role of extracellular vesicles from adipose tissue‐ and bone marrow‐mesenchymal stromal cells in endothelial proliferation and chondrogenesis

Abstract The secretome of mesenchymal stromal cells (MSCs) derived from different tissue sources is considered an innovative therapeutic tool for regenerative medicine. Although adipose tissue‐and bone marrow‐derived MSCs (ADSCs and BMSCs, respectively) share many biological features, the different...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cansu Gorgun, Maria Elisabetta Federica Palamà, Daniele Reverberi, Maria Cristina Gagliani, Katia Cortese, Roberta Tasso, Chiara Gentili
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	cartilage and bone development ex vivo metatarsal culture model extracellular vesicles mesenchymal stromal cells regenerative medicine Medicine (General) R5-920 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/bd560bce184d4dddbc45f57b68cac4a9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bd560bce184d4dddbc45f57b68cac4a9
record_format	dspace
spelling	oai:doaj.org-article:bd560bce184d4dddbc45f57b68cac4a92021-12-03T12:11:46ZRole of extracellular vesicles from adipose tissue‐ and bone marrow‐mesenchymal stromal cells in endothelial proliferation and chondrogenesis2157-65802157-656410.1002/sctm.21-0107https://doaj.org/article/bd560bce184d4dddbc45f57b68cac4a92021-12-01T00:00:00Zhttps://doi.org/10.1002/sctm.21-0107https://doaj.org/toc/2157-6564https://doaj.org/toc/2157-6580Abstract The secretome of mesenchymal stromal cells (MSCs) derived from different tissue sources is considered an innovative therapeutic tool for regenerative medicine. Although adipose tissue‐and bone marrow‐derived MSCs (ADSCs and BMSCs, respectively) share many biological features, the different tissue origins can be mirrored by variations in their secretory profile, and in particular in the secreted extracellular vesicles (EVs). In this study, we carried out a detailed and comparative characterization of middle‐ and small‐sized EVs (mEVs and sEVs, respectively) released by either ADSCs or BMSCs. Their involvement in an endochondral ossification setting was investigated using ex vivo metatarsal culture models that allowed to explore both blood vessel sprouting and bone growth plate dynamics. Although EVs separated from both cell sources presented similar characteristics in terms of size, concentration, and marker expression, they exhibited different characteristics in terms of protein content and functional effects. ADSC‐EVs overexpressed pro‐angiogenic factors in comparison to the BMSC‐counterpart, and, consequently, they were able to induce a significant increase in endothelial cord outgrowth. On the other hand, BMSC‐EVs contained a higher amount of pro‐differentiation and chemotactic proteins, and they were able to prompt growth plate organization. The present study highlights the importance of selecting the appropriate cell source of EVs for targeted therapeutic applications.Cansu GorgunMaria Elisabetta Federica PalamàDaniele ReverberiMaria Cristina GaglianiKatia CorteseRoberta TassoChiara GentiliWileyarticlecartilage and bone developmentex vivo metatarsal culture modelextracellular vesiclesmesenchymal stromal cellsregenerative medicineMedicine (General)R5-920CytologyQH573-671ENStem Cells Translational Medicine, Vol 10, Iss 12, Pp 1680-1695 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cartilage and bone development ex vivo metatarsal culture model extracellular vesicles mesenchymal stromal cells regenerative medicine Medicine (General) R5-920 Cytology QH573-671
spellingShingle	cartilage and bone development ex vivo metatarsal culture model extracellular vesicles mesenchymal stromal cells regenerative medicine Medicine (General) R5-920 Cytology QH573-671 Cansu Gorgun Maria Elisabetta Federica Palamà Daniele Reverberi Maria Cristina Gagliani Katia Cortese Roberta Tasso Chiara Gentili Role of extracellular vesicles from adipose tissue‐ and bone marrow‐mesenchymal stromal cells in endothelial proliferation and chondrogenesis
description	Abstract The secretome of mesenchymal stromal cells (MSCs) derived from different tissue sources is considered an innovative therapeutic tool for regenerative medicine. Although adipose tissue‐and bone marrow‐derived MSCs (ADSCs and BMSCs, respectively) share many biological features, the different tissue origins can be mirrored by variations in their secretory profile, and in particular in the secreted extracellular vesicles (EVs). In this study, we carried out a detailed and comparative characterization of middle‐ and small‐sized EVs (mEVs and sEVs, respectively) released by either ADSCs or BMSCs. Their involvement in an endochondral ossification setting was investigated using ex vivo metatarsal culture models that allowed to explore both blood vessel sprouting and bone growth plate dynamics. Although EVs separated from both cell sources presented similar characteristics in terms of size, concentration, and marker expression, they exhibited different characteristics in terms of protein content and functional effects. ADSC‐EVs overexpressed pro‐angiogenic factors in comparison to the BMSC‐counterpart, and, consequently, they were able to induce a significant increase in endothelial cord outgrowth. On the other hand, BMSC‐EVs contained a higher amount of pro‐differentiation and chemotactic proteins, and they were able to prompt growth plate organization. The present study highlights the importance of selecting the appropriate cell source of EVs for targeted therapeutic applications.
format	article
author	Cansu Gorgun Maria Elisabetta Federica Palamà Daniele Reverberi Maria Cristina Gagliani Katia Cortese Roberta Tasso Chiara Gentili
author_facet	Cansu Gorgun Maria Elisabetta Federica Palamà Daniele Reverberi Maria Cristina Gagliani Katia Cortese Roberta Tasso Chiara Gentili
author_sort	Cansu Gorgun
title	Role of extracellular vesicles from adipose tissue‐ and bone marrow‐mesenchymal stromal cells in endothelial proliferation and chondrogenesis
title_short	Role of extracellular vesicles from adipose tissue‐ and bone marrow‐mesenchymal stromal cells in endothelial proliferation and chondrogenesis
title_full	Role of extracellular vesicles from adipose tissue‐ and bone marrow‐mesenchymal stromal cells in endothelial proliferation and chondrogenesis
title_fullStr	Role of extracellular vesicles from adipose tissue‐ and bone marrow‐mesenchymal stromal cells in endothelial proliferation and chondrogenesis
title_full_unstemmed	Role of extracellular vesicles from adipose tissue‐ and bone marrow‐mesenchymal stromal cells in endothelial proliferation and chondrogenesis
title_sort	role of extracellular vesicles from adipose tissue‐ and bone marrow‐mesenchymal stromal cells in endothelial proliferation and chondrogenesis
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/bd560bce184d4dddbc45f57b68cac4a9
work_keys_str_mv	AT cansugorgun roleofextracellularvesiclesfromadiposetissueandbonemarrowmesenchymalstromalcellsinendothelialproliferationandchondrogenesis AT mariaelisabettafedericapalama roleofextracellularvesiclesfromadiposetissueandbonemarrowmesenchymalstromalcellsinendothelialproliferationandchondrogenesis AT danielereverberi roleofextracellularvesiclesfromadiposetissueandbonemarrowmesenchymalstromalcellsinendothelialproliferationandchondrogenesis AT mariacristinagagliani roleofextracellularvesiclesfromadiposetissueandbonemarrowmesenchymalstromalcellsinendothelialproliferationandchondrogenesis AT katiacortese roleofextracellularvesiclesfromadiposetissueandbonemarrowmesenchymalstromalcellsinendothelialproliferationandchondrogenesis AT robertatasso roleofextracellularvesiclesfromadiposetissueandbonemarrowmesenchymalstromalcellsinendothelialproliferationandchondrogenesis AT chiaragentili roleofextracellularvesiclesfromadiposetissueandbonemarrowmesenchymalstromalcellsinendothelialproliferationandchondrogenesis
_version_	1718373292043141120

Role of extracellular vesicles from adipose tissue‐ and bone marrow‐mesenchymal stromal cells in endothelial proliferation and chondrogenesis

Ejemplares similares