Classical Swine Fever: Active Immunisation of Piglets with Subunit (E2) Vaccine in the Presence of Different Levels of Colostral Immunity (China Strain)

The aim of this study was to investigate the efficacy of the subunit vaccine against virulent CSF infection in piglets deriving from sows vaccinated with China strain. The experimental research included 34 piglets aged 45 days (13 naïve and 21 piglets originating from sows immunized with China strai...

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Autores principales:	Jasna Prodanov-Radulović, Radoslav Došen, Vladimir Polaček, Tamaš Petrović, Igor Stojanov, Radomir Ratajac, Miroslav Valčić
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2014
Materias:	classical swine fever colostral immunity subunit e2 vaccines Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/bd56195f32624d869e1d26dccc613e36
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spelling	oai:doaj.org-article:bd56195f32624d869e1d26dccc613e362021-11-17T21:27:52ZClassical Swine Fever: Active Immunisation of Piglets with Subunit (E2) Vaccine in the Presence of Different Levels of Colostral Immunity (China Strain)1820-744810.2478/acve-2014-0046https://doaj.org/article/bd56195f32624d869e1d26dccc613e362014-12-01T00:00:00Zhttps://doi.org/10.2478/acve-2014-0046https://doaj.org/toc/1820-7448The aim of this study was to investigate the efficacy of the subunit vaccine against virulent CSF infection in piglets deriving from sows vaccinated with China strain. The experimental research included 34 piglets aged 45 days (13 naïve and 21 piglets originating from sows immunized with China strain CSFV). Three experimental groups consisting of seven animals each were formed based on serological examination of piglets aged 40 days. At the age of 45 days, the piglets were vaccinated with a subunit vaccine. After revaccination, the piglets were challenged with a virulent CSFV strain. With the aim of controlling virus shedding, two susceptible piglets were introduced into each group. After challenge, clinical monitoring of animals was performed, and blood samples were obtained to detect viremia and the presence of antibodies against CSF. The control of CSFV shedding by vaccinated, artificially infected piglets was performed by RT-PCR analysis of oropharyngeal and rectal swabs. After death or sacrifice of the animals, autopsy was performed along with the gross pathological examination and tissue sampling with the purpose of determining the presence and distribution of CSFV (ELISA and RT-PCR). Application of the subunit vaccine in piglets originating from sows vaccinated with China-strain resulted in a good active immune response. Following challenge virus shedding was confirmed, but without contact infection in susceptible animals in cohabitation. The results indicate that the subunit vaccine may have a potential application in the control of CSF in enzootic regions.Jasna Prodanov-RadulovićRadoslav DošenVladimir PolačekTamaš PetrovićIgor StojanovRadomir RatajacMiroslav ValčićSciendoarticleclassical swine fevercolostral immunitysubunit e2 vaccinesVeterinary medicineSF600-1100ENActa Veterinaria, Vol 64, Iss 4, Pp 493-509 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	classical swine fever colostral immunity subunit e2 vaccines Veterinary medicine SF600-1100
spellingShingle	classical swine fever colostral immunity subunit e2 vaccines Veterinary medicine SF600-1100 Jasna Prodanov-Radulović Radoslav Došen Vladimir Polaček Tamaš Petrović Igor Stojanov Radomir Ratajac Miroslav Valčić Classical Swine Fever: Active Immunisation of Piglets with Subunit (E2) Vaccine in the Presence of Different Levels of Colostral Immunity (China Strain)
description	The aim of this study was to investigate the efficacy of the subunit vaccine against virulent CSF infection in piglets deriving from sows vaccinated with China strain. The experimental research included 34 piglets aged 45 days (13 naïve and 21 piglets originating from sows immunized with China strain CSFV). Three experimental groups consisting of seven animals each were formed based on serological examination of piglets aged 40 days. At the age of 45 days, the piglets were vaccinated with a subunit vaccine. After revaccination, the piglets were challenged with a virulent CSFV strain. With the aim of controlling virus shedding, two susceptible piglets were introduced into each group. After challenge, clinical monitoring of animals was performed, and blood samples were obtained to detect viremia and the presence of antibodies against CSF. The control of CSFV shedding by vaccinated, artificially infected piglets was performed by RT-PCR analysis of oropharyngeal and rectal swabs. After death or sacrifice of the animals, autopsy was performed along with the gross pathological examination and tissue sampling with the purpose of determining the presence and distribution of CSFV (ELISA and RT-PCR). Application of the subunit vaccine in piglets originating from sows vaccinated with China-strain resulted in a good active immune response. Following challenge virus shedding was confirmed, but without contact infection in susceptible animals in cohabitation. The results indicate that the subunit vaccine may have a potential application in the control of CSF in enzootic regions.
format	article
author	Jasna Prodanov-Radulović Radoslav Došen Vladimir Polaček Tamaš Petrović Igor Stojanov Radomir Ratajac Miroslav Valčić
author_facet	Jasna Prodanov-Radulović Radoslav Došen Vladimir Polaček Tamaš Petrović Igor Stojanov Radomir Ratajac Miroslav Valčić
author_sort	Jasna Prodanov-Radulović
title	Classical Swine Fever: Active Immunisation of Piglets with Subunit (E2) Vaccine in the Presence of Different Levels of Colostral Immunity (China Strain)
title_short	Classical Swine Fever: Active Immunisation of Piglets with Subunit (E2) Vaccine in the Presence of Different Levels of Colostral Immunity (China Strain)
title_full	Classical Swine Fever: Active Immunisation of Piglets with Subunit (E2) Vaccine in the Presence of Different Levels of Colostral Immunity (China Strain)
title_fullStr	Classical Swine Fever: Active Immunisation of Piglets with Subunit (E2) Vaccine in the Presence of Different Levels of Colostral Immunity (China Strain)
title_full_unstemmed	Classical Swine Fever: Active Immunisation of Piglets with Subunit (E2) Vaccine in the Presence of Different Levels of Colostral Immunity (China Strain)
title_sort	classical swine fever: active immunisation of piglets with subunit (e2) vaccine in the presence of different levels of colostral immunity (china strain)
publisher	Sciendo
publishDate	2014
url	https://doaj.org/article/bd56195f32624d869e1d26dccc613e36
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Classical Swine Fever: Active Immunisation of Piglets with Subunit (E2) Vaccine in the Presence of Different Levels of Colostral Immunity (China Strain)

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