Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients

Turner syndrome (TS) is a genetic disorder in females with X Chromosome monosomy associated with highly variable clinical features, including premature primary gonadal failure leading to ovarian dysfunction and infertility. The mechanism of development of primordial germ cells (PGCs) and their conne...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Aline Fernanda de Souza, Fabiana Fernandes Bressan, Naira Caroline Godoy Pieri, Ramon Cesar Botigelli, Tamas Revay, Simone Kashima Haddad, Dimas Tadeu Covas, Ester Silveira Ramos, Willian Allan King, Flavio Vieira Meirelles
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Turner syndrome iPSCs PGCLCs Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bd63ec6557c94184bad4ea841cd1c3ab
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bd63ec6557c94184bad4ea841cd1c3ab
record_format	dspace
spelling	oai:doaj.org-article:bd63ec6557c94184bad4ea841cd1c3ab2021-11-25T17:11:30ZGeneration of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients10.3390/cells101130992073-4409https://doaj.org/article/bd63ec6557c94184bad4ea841cd1c3ab2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3099https://doaj.org/toc/2073-4409Turner syndrome (TS) is a genetic disorder in females with X Chromosome monosomy associated with highly variable clinical features, including premature primary gonadal failure leading to ovarian dysfunction and infertility. The mechanism of development of primordial germ cells (PGCs) and their connection with ovarian failure in TS is poorly understood. An in vitro model of PGCs from TS would be beneficial for investigating genetic and epigenetic factors that influence germ cell specification. Here we investigated the potential of reprogramming peripheral mononuclear blood cells from TS women (PBMCs-TS) into iPSCs following in vitro differentiation in hPGCLCs. All hiPSCs-TS lines demonstrated pluripotency state and were capable of differentiation into three embryonic layers (ectoderm, endoderm, and mesoderm). The PGCLCs-TS recapitulated the initial germline development period regarding transcripts and protein marks, including the epigenetic profile. Overall, our results highlighted the feasibility of producing in vitro models to help the understanding of the mechanisms associated with germ cell formation in TS.Aline Fernanda de SouzaFabiana Fernandes BressanNaira Caroline Godoy PieriRamon Cesar BotigelliTamas RevaySimone Kashima HaddadDimas Tadeu CovasEster Silveira RamosWillian Allan KingFlavio Vieira MeirellesMDPI AGarticleTurner syndromeiPSCsPGCLCsBiology (General)QH301-705.5ENCells, Vol 10, Iss 3099, p 3099 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Turner syndrome iPSCs PGCLCs Biology (General) QH301-705.5
spellingShingle	Turner syndrome iPSCs PGCLCs Biology (General) QH301-705.5 Aline Fernanda de Souza Fabiana Fernandes Bressan Naira Caroline Godoy Pieri Ramon Cesar Botigelli Tamas Revay Simone Kashima Haddad Dimas Tadeu Covas Ester Silveira Ramos Willian Allan King Flavio Vieira Meirelles Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients
description	Turner syndrome (TS) is a genetic disorder in females with X Chromosome monosomy associated with highly variable clinical features, including premature primary gonadal failure leading to ovarian dysfunction and infertility. The mechanism of development of primordial germ cells (PGCs) and their connection with ovarian failure in TS is poorly understood. An in vitro model of PGCs from TS would be beneficial for investigating genetic and epigenetic factors that influence germ cell specification. Here we investigated the potential of reprogramming peripheral mononuclear blood cells from TS women (PBMCs-TS) into iPSCs following in vitro differentiation in hPGCLCs. All hiPSCs-TS lines demonstrated pluripotency state and were capable of differentiation into three embryonic layers (ectoderm, endoderm, and mesoderm). The PGCLCs-TS recapitulated the initial germline development period regarding transcripts and protein marks, including the epigenetic profile. Overall, our results highlighted the feasibility of producing in vitro models to help the understanding of the mechanisms associated with germ cell formation in TS.
format	article
author	Aline Fernanda de Souza Fabiana Fernandes Bressan Naira Caroline Godoy Pieri Ramon Cesar Botigelli Tamas Revay Simone Kashima Haddad Dimas Tadeu Covas Ester Silveira Ramos Willian Allan King Flavio Vieira Meirelles
author_facet	Aline Fernanda de Souza Fabiana Fernandes Bressan Naira Caroline Godoy Pieri Ramon Cesar Botigelli Tamas Revay Simone Kashima Haddad Dimas Tadeu Covas Ester Silveira Ramos Willian Allan King Flavio Vieira Meirelles
author_sort	Aline Fernanda de Souza
title	Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients
title_short	Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients
title_full	Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients
title_fullStr	Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients
title_full_unstemmed	Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients
title_sort	generation of primordial germ cell-like cells from ipscs derived from turner syndrome patients
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bd63ec6557c94184bad4ea841cd1c3ab
work_keys_str_mv	AT alinefernandadesouza generationofprimordialgermcelllikecellsfromipscsderivedfromturnersyndromepatients AT fabianafernandesbressan generationofprimordialgermcelllikecellsfromipscsderivedfromturnersyndromepatients AT nairacarolinegodoypieri generationofprimordialgermcelllikecellsfromipscsderivedfromturnersyndromepatients AT ramoncesarbotigelli generationofprimordialgermcelllikecellsfromipscsderivedfromturnersyndromepatients AT tamasrevay generationofprimordialgermcelllikecellsfromipscsderivedfromturnersyndromepatients AT simonekashimahaddad generationofprimordialgermcelllikecellsfromipscsderivedfromturnersyndromepatients AT dimastadeucovas generationofprimordialgermcelllikecellsfromipscsderivedfromturnersyndromepatients AT estersilveiraramos generationofprimordialgermcelllikecellsfromipscsderivedfromturnersyndromepatients AT willianallanking generationofprimordialgermcelllikecellsfromipscsderivedfromturnersyndromepatients AT flaviovieirameirelles generationofprimordialgermcelllikecellsfromipscsderivedfromturnersyndromepatients
_version_	1718412674608398336

Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients

Ejemplares similares