The Role of endoplasmic reticulum metallo protease 1 on Autophagy Pathway in HCT-116 Colorectal Cancer Cell Line

Background: Autophagy and unfolded protein response (UPR) are mechanisms with dual roles in both maintaining the cellular homeostasis and progression of various diseases such as cancer. Therefore, identification of different molecules and proteins involved in the regulation of these pathways may con...

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Autores principales:	Mozhdeh Zamani, Sanaz Dastghaib, Mehran Erfani, Seyed Vahid Hosseini, Pooneh Mokarram
Formato:	article
Lenguaje:	EN
Publicado:	Shiraz University of Medical Sciences 2021
Materias:	ermp1 colorectal neoplasm target therapy endoplasmic reticulum stress Medicine R
Acceso en línea:	https://doaj.org/article/bd64c61cae1f425e9236a4357b5b8a24
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spelling	oai:doaj.org-article:bd64c61cae1f425e9236a4357b5b8a242021-11-14T06:50:42ZThe Role of endoplasmic reticulum metallo protease 1 on Autophagy Pathway in HCT-116 Colorectal Cancer Cell Line2783-243010.30476/acrr.2021.91373.1102https://doaj.org/article/bd64c61cae1f425e9236a4357b5b8a242021-06-01T00:00:00Zhttps://colorectalresearch.sums.ac.ir/article_47640_aea27b9ced7d5fabbf87fd7c0ec75c52.pdfhttps://doaj.org/toc/2783-2430Background: Autophagy and unfolded protein response (UPR) are mechanisms with dual roles in both maintaining the cellular homeostasis and progression of various diseases such as cancer. Therefore, identification of different molecules and proteins involved in the regulation of these pathways may contribute to find new therapeutic targets. A member of the M28 family of the metallopeptidases, Endoplasmic Reticulum Metallo Protease 1 (ERMP1), is overexpressed in cancers such as colorectal cancer. The role of this protein in the UPR activation was previously reported in breast cancer. We aimed to evaluate the role of ERMP1 in the activation of autophagy and apoptosis in colorectal cancer. Methods: ERMP1 Gene silencing was performed using specific small hairpin RNA (shRNA) in HCT-116 colorectal cancer cell line. Then, autophagy associated protein markers including Beclin 1, p62 and LC3II were evaluated using western blot. The effect of ERMP1 knockdown on cellular apoptosis was also assessed by propidium iodide staining flow cytometry analysis. Statistical analysis was performed using SPSS software version 20. Results: All three autophagy markers were increased significantly in the ERMP1-silenced HCT116 cell lines compared with negative control cells (P < 0.05). It seems that ERMP1 silencing inhibits autophagy at the flux stage. However, ERMP1 knockdown had no significant effect on HCT-116 apoptotic cell death (P > 0.05). Conclusion: The oncogenic protein, ERMP1, activates autophagy in colorectal cancer cell line. Targeting of ERMP1 may be considered as a proper approach in colorectal cancer therapy. Further investigations are required to confirm these results.Mozhdeh ZamaniSanaz DastghaibMehran ErfaniSeyed Vahid HosseiniPooneh MokarramShiraz University of Medical Sciencesarticleermp1colorectal neoplasmtarget therapyendoplasmic reticulum stressMedicineRENIranian Journal of Colorectal Research, Vol 9, Iss 2, Pp 63-68 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ermp1 colorectal neoplasm target therapy endoplasmic reticulum stress Medicine R
spellingShingle	ermp1 colorectal neoplasm target therapy endoplasmic reticulum stress Medicine R Mozhdeh Zamani Sanaz Dastghaib Mehran Erfani Seyed Vahid Hosseini Pooneh Mokarram The Role of endoplasmic reticulum metallo protease 1 on Autophagy Pathway in HCT-116 Colorectal Cancer Cell Line
description	Background: Autophagy and unfolded protein response (UPR) are mechanisms with dual roles in both maintaining the cellular homeostasis and progression of various diseases such as cancer. Therefore, identification of different molecules and proteins involved in the regulation of these pathways may contribute to find new therapeutic targets. A member of the M28 family of the metallopeptidases, Endoplasmic Reticulum Metallo Protease 1 (ERMP1), is overexpressed in cancers such as colorectal cancer. The role of this protein in the UPR activation was previously reported in breast cancer. We aimed to evaluate the role of ERMP1 in the activation of autophagy and apoptosis in colorectal cancer. Methods: ERMP1 Gene silencing was performed using specific small hairpin RNA (shRNA) in HCT-116 colorectal cancer cell line. Then, autophagy associated protein markers including Beclin 1, p62 and LC3II were evaluated using western blot. The effect of ERMP1 knockdown on cellular apoptosis was also assessed by propidium iodide staining flow cytometry analysis. Statistical analysis was performed using SPSS software version 20. Results: All three autophagy markers were increased significantly in the ERMP1-silenced HCT116 cell lines compared with negative control cells (P < 0.05). It seems that ERMP1 silencing inhibits autophagy at the flux stage. However, ERMP1 knockdown had no significant effect on HCT-116 apoptotic cell death (P > 0.05). Conclusion: The oncogenic protein, ERMP1, activates autophagy in colorectal cancer cell line. Targeting of ERMP1 may be considered as a proper approach in colorectal cancer therapy. Further investigations are required to confirm these results.
format	article
author	Mozhdeh Zamani Sanaz Dastghaib Mehran Erfani Seyed Vahid Hosseini Pooneh Mokarram
author_facet	Mozhdeh Zamani Sanaz Dastghaib Mehran Erfani Seyed Vahid Hosseini Pooneh Mokarram
author_sort	Mozhdeh Zamani
title	The Role of endoplasmic reticulum metallo protease 1 on Autophagy Pathway in HCT-116 Colorectal Cancer Cell Line
title_short	The Role of endoplasmic reticulum metallo protease 1 on Autophagy Pathway in HCT-116 Colorectal Cancer Cell Line
title_full	The Role of endoplasmic reticulum metallo protease 1 on Autophagy Pathway in HCT-116 Colorectal Cancer Cell Line
title_fullStr	The Role of endoplasmic reticulum metallo protease 1 on Autophagy Pathway in HCT-116 Colorectal Cancer Cell Line
title_full_unstemmed	The Role of endoplasmic reticulum metallo protease 1 on Autophagy Pathway in HCT-116 Colorectal Cancer Cell Line
title_sort	role of endoplasmic reticulum metallo protease 1 on autophagy pathway in hct-116 colorectal cancer cell line
publisher	Shiraz University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/bd64c61cae1f425e9236a4357b5b8a24
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The Role of endoplasmic reticulum metallo protease 1 on Autophagy Pathway in HCT-116 Colorectal Cancer Cell Line

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