Evaluation of microRNA expression in a sheep model for lung fibrosis

Abstract Background Idiopathic pulmonary fibrosis (IPF) is a chronic progressive fibroproliferative disorder that has one of the poorest prognoses amongst interstitial lung diseases. Recently, the finding of aberrant expression levels of miRNAs in IPF patients has drawn significant attention to the...

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Autores principales:	Udari Eshani Perera, Habtamu B. Derseh, Sasika N. V. Dewage, Andrew Stent, Rukmali Wijayarathna, Kenneth J. Snibson
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Sheep model microRNA Idiopathic pulmonary fibrosis Bleomycin Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/bd64d6e6b5a24b0698ab269167819e9e
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spelling	oai:doaj.org-article:bd64d6e6b5a24b0698ab269167819e9e2021-11-21T12:26:33ZEvaluation of microRNA expression in a sheep model for lung fibrosis10.1186/s12864-021-08073-41471-2164https://doaj.org/article/bd64d6e6b5a24b0698ab269167819e9e2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08073-4https://doaj.org/toc/1471-2164Abstract Background Idiopathic pulmonary fibrosis (IPF) is a chronic progressive fibroproliferative disorder that has one of the poorest prognoses amongst interstitial lung diseases. Recently, the finding of aberrant expression levels of miRNAs in IPF patients has drawn significant attention to the involvement of these molecules in the pathogenesis of this disease. Clarification of the differential expression of miRNAs in health and disease may identify novel therapeutic strategies that can be employed in the future to combat IPF. This study evaluates the miRNA expression profiles in a sheep model for lung fibrosis and compares them to the miRNA profiles of both IPF patients and the mouse bleomycin model for pulmonary fibrosis. Pathway enrichment analyses were performed on differentially expressed miRNAs to illustrate which biological mechanisms were associated with lung fibrosis. Results We discovered 49 differentially expressed miRNAs in the sheep fibrosis model, in which 32 miRNAs were significantly down regulated, while 17 miRNAs were significantly upregulated due to bleomycin-induced lung injury. Moreover, the miRNA families miR-29, miR-26, miR-30, let-7, miR-21, miR-19, miR-17 and miR-199 were aberrantly expressed in both sheep and mouse models, with similar differential miRNAs expression observed in IPF cases. Importantly, 18 miRNAs were aberrantly expressed in both the sheep model and IPF patients, but not in mice. Conclusion Together with pathway enrichment analyses, these results show that the sheep model can potentially be used to characterize previously unrecognized biological pathways associated with lung fibrosis.Udari Eshani PereraHabtamu B. DersehSasika N. V. DewageAndrew StentRukmali WijayarathnaKenneth J. SnibsonBMCarticleSheep modelmicroRNAIdiopathic pulmonary fibrosisBleomycinBiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Sheep model microRNA Idiopathic pulmonary fibrosis Bleomycin Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Sheep model microRNA Idiopathic pulmonary fibrosis Bleomycin Biotechnology TP248.13-248.65 Genetics QH426-470 Udari Eshani Perera Habtamu B. Derseh Sasika N. V. Dewage Andrew Stent Rukmali Wijayarathna Kenneth J. Snibson Evaluation of microRNA expression in a sheep model for lung fibrosis
description	Abstract Background Idiopathic pulmonary fibrosis (IPF) is a chronic progressive fibroproliferative disorder that has one of the poorest prognoses amongst interstitial lung diseases. Recently, the finding of aberrant expression levels of miRNAs in IPF patients has drawn significant attention to the involvement of these molecules in the pathogenesis of this disease. Clarification of the differential expression of miRNAs in health and disease may identify novel therapeutic strategies that can be employed in the future to combat IPF. This study evaluates the miRNA expression profiles in a sheep model for lung fibrosis and compares them to the miRNA profiles of both IPF patients and the mouse bleomycin model for pulmonary fibrosis. Pathway enrichment analyses were performed on differentially expressed miRNAs to illustrate which biological mechanisms were associated with lung fibrosis. Results We discovered 49 differentially expressed miRNAs in the sheep fibrosis model, in which 32 miRNAs were significantly down regulated, while 17 miRNAs were significantly upregulated due to bleomycin-induced lung injury. Moreover, the miRNA families miR-29, miR-26, miR-30, let-7, miR-21, miR-19, miR-17 and miR-199 were aberrantly expressed in both sheep and mouse models, with similar differential miRNAs expression observed in IPF cases. Importantly, 18 miRNAs were aberrantly expressed in both the sheep model and IPF patients, but not in mice. Conclusion Together with pathway enrichment analyses, these results show that the sheep model can potentially be used to characterize previously unrecognized biological pathways associated with lung fibrosis.
format	article
author	Udari Eshani Perera Habtamu B. Derseh Sasika N. V. Dewage Andrew Stent Rukmali Wijayarathna Kenneth J. Snibson
author_facet	Udari Eshani Perera Habtamu B. Derseh Sasika N. V. Dewage Andrew Stent Rukmali Wijayarathna Kenneth J. Snibson
author_sort	Udari Eshani Perera
title	Evaluation of microRNA expression in a sheep model for lung fibrosis
title_short	Evaluation of microRNA expression in a sheep model for lung fibrosis
title_full	Evaluation of microRNA expression in a sheep model for lung fibrosis
title_fullStr	Evaluation of microRNA expression in a sheep model for lung fibrosis
title_full_unstemmed	Evaluation of microRNA expression in a sheep model for lung fibrosis
title_sort	evaluation of microrna expression in a sheep model for lung fibrosis
publisher	BMC
publishDate	2021
url	https://doaj.org/article/bd64d6e6b5a24b0698ab269167819e9e
work_keys_str_mv	AT udarieshaniperera evaluationofmicrornaexpressioninasheepmodelforlungfibrosis AT habtamubderseh evaluationofmicrornaexpressioninasheepmodelforlungfibrosis AT sasikanvdewage evaluationofmicrornaexpressioninasheepmodelforlungfibrosis AT andrewstent evaluationofmicrornaexpressioninasheepmodelforlungfibrosis AT rukmaliwijayarathna evaluationofmicrornaexpressioninasheepmodelforlungfibrosis AT kennethjsnibson evaluationofmicrornaexpressioninasheepmodelforlungfibrosis
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Evaluation of microRNA expression in a sheep model for lung fibrosis

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