Short Communication: New record of Stenotrophomonas sp. as endosymbiont bacteria in Rhizopus microsporus

Abstract. Virgianti DP, Natalia D, Aryantha INP. 2020. Short Communication: New record of Stenotrophomonas sp. as endosymbiont bacteria in Rhizopus microsporus. Biodiversitas 21: 1678-1685. Rhizopus sp. are closely related to humankind in many aspects of human life. Several species of Rhizopus are i...

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Autores principales:	Dewi Peti Virgianti, Desi Natalia, I Nyoman Pugeg Aryantha
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	bacterial-fungal interaction, bfi, ehb, endo hyphal bacteria, endosymbiont, fish, rhizopus Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bd66d363eb1f4fa090d5837ea2965a54
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spelling	oai:doaj.org-article:bd66d363eb1f4fa090d5837ea2965a542021-11-22T00:34:39ZShort Communication: New record of Stenotrophomonas sp. as endosymbiont bacteria in Rhizopus microsporus1412-033X2085-472210.13057/biodiv/d210449https://doaj.org/article/bd66d363eb1f4fa090d5837ea2965a542020-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5017https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Virgianti DP, Natalia D, Aryantha INP. 2020. Short Communication: New record of Stenotrophomonas sp. as endosymbiont bacteria in Rhizopus microsporus. Biodiversitas 21: 1678-1685. Rhizopus sp. are closely related to humankind in many aspects of human life. Several species of Rhizopus are important in food, agriculture, and health industries. Rhizopus microsporus has endosymbiont bacteria that has been identified as Burkholderia sp. This study aimed to identify the endosymbiont bacteria in R. microsporus isolated from Moringa oleifera Lam leaves. Ring technique, simple method of agar heap technique and antibiotic technique, was conducted to purify the hyphae and to eliminate ectosymbiont and contaminant bacteria outside the hyphae. The presence of endosymbiont bacteria in R. microsporus was determined by using LIVE/DEAD® BacLight Bacterial Viability Kits L13152 and the Fluorescent in Situ Hybridization (FISH) method using EUB338 probes. It was suggested that the culturable endosymbiont bacteria was identified as Stenotrophomonas sp. based on the phylogenetic tree using the 16S rDNADewi Peti VirgiantiDesi NataliaI Nyoman Pugeg AryanthaMBI & UNS Soloarticlebacterial-fungal interaction, bfi, ehb, endo hyphal bacteria, endosymbiont, fish, rhizopusBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacterial-fungal interaction, bfi, ehb, endo hyphal bacteria, endosymbiont, fish, rhizopus Biology (General) QH301-705.5
spellingShingle	bacterial-fungal interaction, bfi, ehb, endo hyphal bacteria, endosymbiont, fish, rhizopus Biology (General) QH301-705.5 Dewi Peti Virgianti Desi Natalia I Nyoman Pugeg Aryantha Short Communication: New record of Stenotrophomonas sp. as endosymbiont bacteria in Rhizopus microsporus
description	Abstract. Virgianti DP, Natalia D, Aryantha INP. 2020. Short Communication: New record of Stenotrophomonas sp. as endosymbiont bacteria in Rhizopus microsporus. Biodiversitas 21: 1678-1685. Rhizopus sp. are closely related to humankind in many aspects of human life. Several species of Rhizopus are important in food, agriculture, and health industries. Rhizopus microsporus has endosymbiont bacteria that has been identified as Burkholderia sp. This study aimed to identify the endosymbiont bacteria in R. microsporus isolated from Moringa oleifera Lam leaves. Ring technique, simple method of agar heap technique and antibiotic technique, was conducted to purify the hyphae and to eliminate ectosymbiont and contaminant bacteria outside the hyphae. The presence of endosymbiont bacteria in R. microsporus was determined by using LIVE/DEAD® BacLight Bacterial Viability Kits L13152 and the Fluorescent in Situ Hybridization (FISH) method using EUB338 probes. It was suggested that the culturable endosymbiont bacteria was identified as Stenotrophomonas sp. based on the phylogenetic tree using the 16S rDNA
format	article
author	Dewi Peti Virgianti Desi Natalia I Nyoman Pugeg Aryantha
author_facet	Dewi Peti Virgianti Desi Natalia I Nyoman Pugeg Aryantha
author_sort	Dewi Peti Virgianti
title	Short Communication: New record of Stenotrophomonas sp. as endosymbiont bacteria in Rhizopus microsporus
title_short	Short Communication: New record of Stenotrophomonas sp. as endosymbiont bacteria in Rhizopus microsporus
title_full	Short Communication: New record of Stenotrophomonas sp. as endosymbiont bacteria in Rhizopus microsporus
title_fullStr	Short Communication: New record of Stenotrophomonas sp. as endosymbiont bacteria in Rhizopus microsporus
title_full_unstemmed	Short Communication: New record of Stenotrophomonas sp. as endosymbiont bacteria in Rhizopus microsporus
title_sort	short communication: new record of stenotrophomonas sp. as endosymbiont bacteria in rhizopus microsporus
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/bd66d363eb1f4fa090d5837ea2965a54
work_keys_str_mv	AT dewipetivirgianti shortcommunicationnewrecordofstenotrophomonasspasendosymbiontbacteriainrhizopusmicrosporus AT desinatalia shortcommunicationnewrecordofstenotrophomonasspasendosymbiontbacteriainrhizopusmicrosporus AT inyomanpugegaryantha shortcommunicationnewrecordofstenotrophomonasspasendosymbiontbacteriainrhizopusmicrosporus
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Short Communication: New record of Stenotrophomonas sp. as endosymbiont bacteria in Rhizopus microsporus

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