Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels

Abstract Cloning, through somatic cell nuclear transfer (SCNT), has the potential for a large expansion of genetically favorable traits in a population in a relatively short term. In the present study we aimed to produce multiple cloned camels from racing, show and dairy exemplars. We compared sever...

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Autores principales:	P. O. Olsson, A. H. Tinson, N. Al Shamsi, K. S. Kuhad, R. Singh, Y. B. Son, Y. Jeong, Y. W. Jeong, L. Cai, K. Sakaguchi, S. Kim, E. J. Choi, X. Yu, W. S. Hwang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bd6c25e5fabd4aa49ced636f523c16b7
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spelling	oai:doaj.org-article:bd6c25e5fabd4aa49ced636f523c16b72021-12-02T16:14:16ZBlastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels10.1038/s41598-021-92465-92045-2322https://doaj.org/article/bd6c25e5fabd4aa49ced636f523c16b72021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92465-9https://doaj.org/toc/2045-2322Abstract Cloning, through somatic cell nuclear transfer (SCNT), has the potential for a large expansion of genetically favorable traits in a population in a relatively short term. In the present study we aimed to produce multiple cloned camels from racing, show and dairy exemplars. We compared several parameters including oocyte source, donor cell and breed differences, transfer methods, embryo formation and pregnancy rates and maintenance following SCNT. We successfully achieved 47 pregnancies, 28 births and 19 cloned offspring who are at present healthy and have developed normally. Here we report cloned camels from surgical embryo transfer and correlate blastocyst formation rates with the ability to achieve pregnancies. We found no difference in the parameters affecting production of clones by camel breed, and show clear differences on oocyte source in cloning outcomes. Taken together we demonstrate that large scale cloning of camels is possible and that further improvements can be achieved.P. O. OlssonA. H. TinsonN. Al ShamsiK. S. KuhadR. SinghY. B. SonY. JeongY. W. JeongL. CaiK. SakaguchiS. KimE. J. ChoiX. YuW. S. HwangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q P. O. Olsson A. H. Tinson N. Al Shamsi K. S. Kuhad R. Singh Y. B. Son Y. Jeong Y. W. Jeong L. Cai K. Sakaguchi S. Kim E. J. Choi X. Yu W. S. Hwang Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels
description	Abstract Cloning, through somatic cell nuclear transfer (SCNT), has the potential for a large expansion of genetically favorable traits in a population in a relatively short term. In the present study we aimed to produce multiple cloned camels from racing, show and dairy exemplars. We compared several parameters including oocyte source, donor cell and breed differences, transfer methods, embryo formation and pregnancy rates and maintenance following SCNT. We successfully achieved 47 pregnancies, 28 births and 19 cloned offspring who are at present healthy and have developed normally. Here we report cloned camels from surgical embryo transfer and correlate blastocyst formation rates with the ability to achieve pregnancies. We found no difference in the parameters affecting production of clones by camel breed, and show clear differences on oocyte source in cloning outcomes. Taken together we demonstrate that large scale cloning of camels is possible and that further improvements can be achieved.
format	article
author	P. O. Olsson A. H. Tinson N. Al Shamsi K. S. Kuhad R. Singh Y. B. Son Y. Jeong Y. W. Jeong L. Cai K. Sakaguchi S. Kim E. J. Choi X. Yu W. S. Hwang
author_facet	P. O. Olsson A. H. Tinson N. Al Shamsi K. S. Kuhad R. Singh Y. B. Son Y. Jeong Y. W. Jeong L. Cai K. Sakaguchi S. Kim E. J. Choi X. Yu W. S. Hwang
author_sort	P. O. Olsson
title	Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels
title_short	Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels
title_full	Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels
title_fullStr	Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels
title_full_unstemmed	Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels
title_sort	blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bd6c25e5fabd4aa49ced636f523c16b7
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Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels

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