A Novel Thromboplastin-Based Rat Model of Ischemic Stroke

The thromboembolic ischemia model is one of the most applicable for studying ischemic stroke in humans. The aim of this study was to develop a novel thromboembolic stroke model, allowing, by affordable tools, to reproduce cerebral infarction in rats. In the experimental group, the left common caroti...

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Autores principales:	Irina V. Ostrova, Sergei N. Kalabushev, Ivan A. Ryzhkov, Zoya I. Tsokolaeva
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	embolic stroke rat model thromboplastin brain injury hippocampus Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/bd73cedeb4124032810714eefb0ede4c
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spelling	oai:doaj.org-article:bd73cedeb4124032810714eefb0ede4c2021-11-25T16:58:05ZA Novel Thromboplastin-Based Rat Model of Ischemic Stroke10.3390/brainsci111114752076-3425https://doaj.org/article/bd73cedeb4124032810714eefb0ede4c2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3425/11/11/1475https://doaj.org/toc/2076-3425The thromboembolic ischemia model is one of the most applicable for studying ischemic stroke in humans. The aim of this study was to develop a novel thromboembolic stroke model, allowing, by affordable tools, to reproduce cerebral infarction in rats. In the experimental group, the left common carotid artery, external carotid artery, and pterygopalatine branch of maxillary artery were ligated. A blood clot that was previously formed (during a 20 min period, in a catheter and syringe, by mixing with a thromboplastin solution and CaCl<sub>2</sub>) was injected into the left internal carotid artery. After 10 min, the catheter was removed, and the incision was sutured. The neurological status of the animals was evaluated using a 20-point scale. Histological examination of brain tissue was performed 6, 24, 72 h, and 6 days post-stroke. All groups showed motor and behavioral disturbances 24 h after surgery, which persisted throughout the study period. A histological examination revealed necrotic foci of varying severity in the cortex and subcortical regions of the ipsilateral hemisphere, for all experimental groups. A decrease in the density of hippocampal pyramidal neurons was revealed. Compared with existing models, the proposed ischemic stroke model significantly reduces surgical time, does not require an expensive operating microscope, and consistently reproduces brain infarction in the area of the middle cerebral artery supply.Irina V. OstrovaSergei N. KalabushevIvan A. RyzhkovZoya I. TsokolaevaMDPI AGarticleembolic strokerat modelthromboplastinbrain injuryhippocampusNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENBrain Sciences, Vol 11, Iss 1475, p 1475 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	embolic stroke rat model thromboplastin brain injury hippocampus Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	embolic stroke rat model thromboplastin brain injury hippocampus Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Irina V. Ostrova Sergei N. Kalabushev Ivan A. Ryzhkov Zoya I. Tsokolaeva A Novel Thromboplastin-Based Rat Model of Ischemic Stroke
description	The thromboembolic ischemia model is one of the most applicable for studying ischemic stroke in humans. The aim of this study was to develop a novel thromboembolic stroke model, allowing, by affordable tools, to reproduce cerebral infarction in rats. In the experimental group, the left common carotid artery, external carotid artery, and pterygopalatine branch of maxillary artery were ligated. A blood clot that was previously formed (during a 20 min period, in a catheter and syringe, by mixing with a thromboplastin solution and CaCl<sub>2</sub>) was injected into the left internal carotid artery. After 10 min, the catheter was removed, and the incision was sutured. The neurological status of the animals was evaluated using a 20-point scale. Histological examination of brain tissue was performed 6, 24, 72 h, and 6 days post-stroke. All groups showed motor and behavioral disturbances 24 h after surgery, which persisted throughout the study period. A histological examination revealed necrotic foci of varying severity in the cortex and subcortical regions of the ipsilateral hemisphere, for all experimental groups. A decrease in the density of hippocampal pyramidal neurons was revealed. Compared with existing models, the proposed ischemic stroke model significantly reduces surgical time, does not require an expensive operating microscope, and consistently reproduces brain infarction in the area of the middle cerebral artery supply.
format	article
author	Irina V. Ostrova Sergei N. Kalabushev Ivan A. Ryzhkov Zoya I. Tsokolaeva
author_facet	Irina V. Ostrova Sergei N. Kalabushev Ivan A. Ryzhkov Zoya I. Tsokolaeva
author_sort	Irina V. Ostrova
title	A Novel Thromboplastin-Based Rat Model of Ischemic Stroke
title_short	A Novel Thromboplastin-Based Rat Model of Ischemic Stroke
title_full	A Novel Thromboplastin-Based Rat Model of Ischemic Stroke
title_fullStr	A Novel Thromboplastin-Based Rat Model of Ischemic Stroke
title_full_unstemmed	A Novel Thromboplastin-Based Rat Model of Ischemic Stroke
title_sort	novel thromboplastin-based rat model of ischemic stroke
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bd73cedeb4124032810714eefb0ede4c
work_keys_str_mv	AT irinavostrova anovelthromboplastinbasedratmodelofischemicstroke AT sergeinkalabushev anovelthromboplastinbasedratmodelofischemicstroke AT ivanaryzhkov anovelthromboplastinbasedratmodelofischemicstroke AT zoyaitsokolaeva anovelthromboplastinbasedratmodelofischemicstroke AT irinavostrova novelthromboplastinbasedratmodelofischemicstroke AT sergeinkalabushev novelthromboplastinbasedratmodelofischemicstroke AT ivanaryzhkov novelthromboplastinbasedratmodelofischemicstroke AT zoyaitsokolaeva novelthromboplastinbasedratmodelofischemicstroke
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A Novel Thromboplastin-Based Rat Model of Ischemic Stroke

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