Accumulation of 4-Deoxy-7-hydroxytrichothecenes, but Not 4,7-Dihydroxytrichothecenes, in Axenic Culture of a Transgenic Nivalenol Chemotype Expressing the NX-Type FgTri1 Gene

Fusarium graminearum species complex produces type B trichothecenes oxygenated at C-7. In axenic liquid culture, F. graminearum mainly accumulates one of the three types of trichothecenes, namely 3-acetyldeoxyinvalenol, 15-acetyldeoxyinvalenol, or mixtures of 4,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kazuyuki Maeda, Yuichi Nakajima, Yoshiaki Koizumi, Naoko Takahashi-Ando, Makoto Kimura, Shuichi Ohsato
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cytochrome P450 monooxygenase gene evolution <i>Fusarium</i> mycotoxin NX-type trichothecenes trichothecene chemotype Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/bd7b386522424c22a3881b962850b524
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bd7b386522424c22a3881b962850b524
record_format	dspace
spelling	oai:doaj.org-article:bd7b386522424c22a3881b962850b5242021-11-11T16:53:53ZAccumulation of 4-Deoxy-7-hydroxytrichothecenes, but Not 4,7-Dihydroxytrichothecenes, in Axenic Culture of a Transgenic Nivalenol Chemotype Expressing the NX-Type <i>FgTri1</i> Gene10.3390/ijms2221114281422-00671661-6596https://doaj.org/article/bd7b386522424c22a3881b962850b5242021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11428https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067<i>Fusarium graminearum</i> species complex produces type B trichothecenes oxygenated at C-7. In axenic liquid culture, <i>F. graminearum</i> mainly accumulates one of the three types of trichothecenes, namely 3-acetyldeoxyinvalenol, 15-acetyldeoxyinvalenol, or mixtures of 4,15-diacetylnivalenol/4-acetylnivalenol, depending on each strain’s genetic background. The acetyl groups of these trichothecenes are slowly deacetylated to give deoxynivalenol (DON) or nivalenol (NIV) on solid medium culture. Due to the evolution of <i>F. graminearum FgTri1</i>, encoding a cytochrome P450 monooxygenase responsible for hydroxylation at both C-7 and C-8, new derivatives of DON, designated as NX-type trichothecenes, have recently emerged. To assess the risks of emergence of new NX-type trichothecenes, we examined the effects of replacing <i>FgTri1</i> in the three chemotypes with <i>FgTri1_<sub>NX chemotype</sub></i>, which encodes a cytochrome P450 monooxygenase that can only hydroxylate C-7 of trichothecenes. Similar to the transgenic DON chemotypes, the transgenic NIV chemotype strain accumulated NX-type 4-deoxytrichothecenes in axenic liquid culture. C-4 oxygenated trichothecenes were marginal, despite the presence of a functional <i>FgTri13</i> encoding a C-4 hydroxylase. At present, outcrossing of the currently occurring NX chemotype with NIV chemotype strains of <i>F. graminearum</i> in the natural environment likely will not yield a new strain that produces a C-4 oxygenated NX-type trichothecene.Kazuyuki MaedaYuichi NakajimaYoshiaki KoizumiNaoko Takahashi-AndoMakoto KimuraShuichi OhsatoMDPI AGarticlecytochrome P450 monooxygenase geneevolution<i>Fusarium</i> mycotoxinNX-type trichothecenestrichothecene chemotypeBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11428, p 11428 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cytochrome P450 monooxygenase gene evolution <i>Fusarium</i> mycotoxin NX-type trichothecenes trichothecene chemotype Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	cytochrome P450 monooxygenase gene evolution <i>Fusarium</i> mycotoxin NX-type trichothecenes trichothecene chemotype Biology (General) QH301-705.5 Chemistry QD1-999 Kazuyuki Maeda Yuichi Nakajima Yoshiaki Koizumi Naoko Takahashi-Ando Makoto Kimura Shuichi Ohsato Accumulation of 4-Deoxy-7-hydroxytrichothecenes, but Not 4,7-Dihydroxytrichothecenes, in Axenic Culture of a Transgenic Nivalenol Chemotype Expressing the NX-Type <i>FgTri1</i> Gene
description	<i>Fusarium graminearum</i> species complex produces type B trichothecenes oxygenated at C-7. In axenic liquid culture, <i>F. graminearum</i> mainly accumulates one of the three types of trichothecenes, namely 3-acetyldeoxyinvalenol, 15-acetyldeoxyinvalenol, or mixtures of 4,15-diacetylnivalenol/4-acetylnivalenol, depending on each strain’s genetic background. The acetyl groups of these trichothecenes are slowly deacetylated to give deoxynivalenol (DON) or nivalenol (NIV) on solid medium culture. Due to the evolution of <i>F. graminearum FgTri1</i>, encoding a cytochrome P450 monooxygenase responsible for hydroxylation at both C-7 and C-8, new derivatives of DON, designated as NX-type trichothecenes, have recently emerged. To assess the risks of emergence of new NX-type trichothecenes, we examined the effects of replacing <i>FgTri1</i> in the three chemotypes with <i>FgTri1_<sub>NX chemotype</sub></i>, which encodes a cytochrome P450 monooxygenase that can only hydroxylate C-7 of trichothecenes. Similar to the transgenic DON chemotypes, the transgenic NIV chemotype strain accumulated NX-type 4-deoxytrichothecenes in axenic liquid culture. C-4 oxygenated trichothecenes were marginal, despite the presence of a functional <i>FgTri13</i> encoding a C-4 hydroxylase. At present, outcrossing of the currently occurring NX chemotype with NIV chemotype strains of <i>F. graminearum</i> in the natural environment likely will not yield a new strain that produces a C-4 oxygenated NX-type trichothecene.
format	article
author	Kazuyuki Maeda Yuichi Nakajima Yoshiaki Koizumi Naoko Takahashi-Ando Makoto Kimura Shuichi Ohsato
author_facet	Kazuyuki Maeda Yuichi Nakajima Yoshiaki Koizumi Naoko Takahashi-Ando Makoto Kimura Shuichi Ohsato
author_sort	Kazuyuki Maeda
title	Accumulation of 4-Deoxy-7-hydroxytrichothecenes, but Not 4,7-Dihydroxytrichothecenes, in Axenic Culture of a Transgenic Nivalenol Chemotype Expressing the NX-Type <i>FgTri1</i> Gene
title_short	Accumulation of 4-Deoxy-7-hydroxytrichothecenes, but Not 4,7-Dihydroxytrichothecenes, in Axenic Culture of a Transgenic Nivalenol Chemotype Expressing the NX-Type <i>FgTri1</i> Gene
title_full	Accumulation of 4-Deoxy-7-hydroxytrichothecenes, but Not 4,7-Dihydroxytrichothecenes, in Axenic Culture of a Transgenic Nivalenol Chemotype Expressing the NX-Type <i>FgTri1</i> Gene
title_fullStr	Accumulation of 4-Deoxy-7-hydroxytrichothecenes, but Not 4,7-Dihydroxytrichothecenes, in Axenic Culture of a Transgenic Nivalenol Chemotype Expressing the NX-Type <i>FgTri1</i> Gene
title_full_unstemmed	Accumulation of 4-Deoxy-7-hydroxytrichothecenes, but Not 4,7-Dihydroxytrichothecenes, in Axenic Culture of a Transgenic Nivalenol Chemotype Expressing the NX-Type <i>FgTri1</i> Gene
title_sort	accumulation of 4-deoxy-7-hydroxytrichothecenes, but not 4,7-dihydroxytrichothecenes, in axenic culture of a transgenic nivalenol chemotype expressing the nx-type <i>fgtri1</i> gene
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bd7b386522424c22a3881b962850b524
work_keys_str_mv	AT kazuyukimaeda accumulationof4deoxy7hydroxytrichothecenesbutnot47dihydroxytrichothecenesinaxeniccultureofatransgenicnivalenolchemotypeexpressingthenxtypeifgtri1igene AT yuichinakajima accumulationof4deoxy7hydroxytrichothecenesbutnot47dihydroxytrichothecenesinaxeniccultureofatransgenicnivalenolchemotypeexpressingthenxtypeifgtri1igene AT yoshiakikoizumi accumulationof4deoxy7hydroxytrichothecenesbutnot47dihydroxytrichothecenesinaxeniccultureofatransgenicnivalenolchemotypeexpressingthenxtypeifgtri1igene AT naokotakahashiando accumulationof4deoxy7hydroxytrichothecenesbutnot47dihydroxytrichothecenesinaxeniccultureofatransgenicnivalenolchemotypeexpressingthenxtypeifgtri1igene AT makotokimura accumulationof4deoxy7hydroxytrichothecenesbutnot47dihydroxytrichothecenesinaxeniccultureofatransgenicnivalenolchemotypeexpressingthenxtypeifgtri1igene AT shuichiohsato accumulationof4deoxy7hydroxytrichothecenesbutnot47dihydroxytrichothecenesinaxeniccultureofatransgenicnivalenolchemotypeexpressingthenxtypeifgtri1igene
_version_	1718432262573260800

Accumulation of 4-Deoxy-7-hydroxytrichothecenes, but Not 4,7-Dihydroxytrichothecenes, in Axenic Culture of a Transgenic Nivalenol Chemotype Expressing the NX-Type <i>FgTri1</i> Gene

Ejemplares similares