Nanostructured model implants for in vivo studies: influence of well-defined nanotopography on de novo bone formation on titanium implants

Ahmed Ballo1,3, Hossein Agheli2,3, Jukka Lausmaa4, Peter Thomsen1,3, Sarunas Petronis2,31Department of Biomaterials, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; 2Applied Physics, Chalmers University of Technology, Gothenburg, Sweden; 3BIOMATCELL, VINN Excellence Center of Biom...

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Autores principales:	Petronis S, Thomsen P, Lausmaa J, Agheli H, Ballo A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bd83aa0b0a934f2da45ed061b09e7da1
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spelling	oai:doaj.org-article:bd83aa0b0a934f2da45ed061b09e7da12021-12-02T02:42:28ZNanostructured model implants for in vivo studies: influence of well-defined nanotopography on de novo bone formation on titanium implants1176-91141178-2013https://doaj.org/article/bd83aa0b0a934f2da45ed061b09e7da12011-12-01T00:00:00Zhttp://www.dovepress.com/nanostructured-model-implants-for-in-vivo-studies-influence-of-well-de-a8910https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Ahmed Ballo1,3, Hossein Agheli2,3, Jukka Lausmaa4, Peter Thomsen1,3, Sarunas Petronis2,31Department of Biomaterials, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; 2Applied Physics, Chalmers University of Technology, Gothenburg, Sweden; 3BIOMATCELL, VINN Excellence Center of Biomaterials and Cell Therapy, Gothenburg, Sweden; 4Department of Chemistry and Materials Technology, SP Technical Research Institute of Sweden, Bor&aring;s, SwedenAbstract: An implantable model system was developed to investigate the effects of nanoscale surface properties on the osseointegration of titanium implants in rat tibia. Topographical nanostructures with a well-defined shape (semispherical protrusions) and variable size (60 nm, 120 nm and 220 nm) were produced by colloidal lithography on the machined implants. Furthermore, the implants were sputter-coated with titanium to ensure a uniform surface chemical composition. The histological evaluation of bone around the implants at 7 days and 28 days after implantation was performed on the ground sections using optical and scanning electron microscopy. Differences between groups were found mainly in the new bone formation process in the endosteal and marrow bone compartments after 28 days of implantation. Implant surfaces with 60 nm features demonstrated significantly higher bone-implant contact (BIC, 76%) compared with the 120 nm (45%) and control (57%) surfaces. This effect was correlated to the higher density and curvature of the 60 nm protrusions. Within the developed model system, nanoscale protrusions could be applied and systematically varied in size in the presence of microscale background roughness on complex screw-shaped implants. Moreover, the model can be adapted for the systematic variation of surface nanofeature density and chemistry, which opens up new possibilities for in vivo studies of various nanoscale surface-bone interactions.Keywords: in vivo, nanotopography, osseointegration, titanium implant, colloidal lithographyPetronis SThomsen PLausmaa JAgheli HBallo ADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 3415-3428 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Petronis S Thomsen P Lausmaa J Agheli H Ballo A Nanostructured model implants for in vivo studies: influence of well-defined nanotopography on de novo bone formation on titanium implants
description	Ahmed Ballo1,3, Hossein Agheli2,3, Jukka Lausmaa4, Peter Thomsen1,3, Sarunas Petronis2,31Department of Biomaterials, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; 2Applied Physics, Chalmers University of Technology, Gothenburg, Sweden; 3BIOMATCELL, VINN Excellence Center of Biomaterials and Cell Therapy, Gothenburg, Sweden; 4Department of Chemistry and Materials Technology, SP Technical Research Institute of Sweden, Bor&aring;s, SwedenAbstract: An implantable model system was developed to investigate the effects of nanoscale surface properties on the osseointegration of titanium implants in rat tibia. Topographical nanostructures with a well-defined shape (semispherical protrusions) and variable size (60 nm, 120 nm and 220 nm) were produced by colloidal lithography on the machined implants. Furthermore, the implants were sputter-coated with titanium to ensure a uniform surface chemical composition. The histological evaluation of bone around the implants at 7 days and 28 days after implantation was performed on the ground sections using optical and scanning electron microscopy. Differences between groups were found mainly in the new bone formation process in the endosteal and marrow bone compartments after 28 days of implantation. Implant surfaces with 60 nm features demonstrated significantly higher bone-implant contact (BIC, 76%) compared with the 120 nm (45%) and control (57%) surfaces. This effect was correlated to the higher density and curvature of the 60 nm protrusions. Within the developed model system, nanoscale protrusions could be applied and systematically varied in size in the presence of microscale background roughness on complex screw-shaped implants. Moreover, the model can be adapted for the systematic variation of surface nanofeature density and chemistry, which opens up new possibilities for in vivo studies of various nanoscale surface-bone interactions.Keywords: in vivo, nanotopography, osseointegration, titanium implant, colloidal lithography
format	article
author	Petronis S Thomsen P Lausmaa J Agheli H Ballo A
author_facet	Petronis S Thomsen P Lausmaa J Agheli H Ballo A
author_sort	Petronis S
title	Nanostructured model implants for in vivo studies: influence of well-defined nanotopography on de novo bone formation on titanium implants
title_short	Nanostructured model implants for in vivo studies: influence of well-defined nanotopography on de novo bone formation on titanium implants
title_full	Nanostructured model implants for in vivo studies: influence of well-defined nanotopography on de novo bone formation on titanium implants
title_fullStr	Nanostructured model implants for in vivo studies: influence of well-defined nanotopography on de novo bone formation on titanium implants
title_full_unstemmed	Nanostructured model implants for in vivo studies: influence of well-defined nanotopography on de novo bone formation on titanium implants
title_sort	nanostructured model implants for in vivo studies: influence of well-defined nanotopography on de novo bone formation on titanium implants
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/bd83aa0b0a934f2da45ed061b09e7da1
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Nanostructured model implants for in vivo studies: influence of well-defined nanotopography on de novo bone formation on titanium implants

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