Kinetics of Eosinophils during Development of the Cellular Infiltrate Surrounding the Nurse Cell of <i>Trichinella spiralis</i> in Experimentally Infected Mice
We study the kinetics of eosinophils during the development of the cellular infiltrate surrounding the nurse cell of <i>Trichinella spiralis</i> (<i>T. spiralis</i>) in experimentally infected mice. Male CD1 mice were experimentally infected with 50 viable muscle larvae of th...
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2021
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oai:doaj.org-article:bd83b1e07d1645899293585b9959a5b12021-11-25T18:37:48ZKinetics of Eosinophils during Development of the Cellular Infiltrate Surrounding the Nurse Cell of <i>Trichinella spiralis</i> in Experimentally Infected Mice10.3390/pathogens101113822076-0817https://doaj.org/article/bd83b1e07d1645899293585b9959a5b12021-10-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1382https://doaj.org/toc/2076-0817We study the kinetics of eosinophils during the development of the cellular infiltrate surrounding the nurse cell of <i>Trichinella spiralis</i> (<i>T. spiralis</i>) in experimentally infected mice. Male CD1 mice were experimentally infected with 50 viable muscle larvae of the MSUS/MEX/91/CM-91 <i>T. spiralis</i> strain. Tongues and diaphragms were obtained daily from days 13 to 39 post infection. Diaphragms were compressed and subjected to Giemsa stain. Tongues were histologically sectioned and stained with erythrosine B or hematoxylin and eosin. The cellular infiltrate and the nurse cell-larva complex were detected by optical microscopy since day 16 post infection. The size of the larva increased exponentially during the course of the infection. The kinetics of eosinophils showed a multimodal trend, with a bimodal predominance. The maximum peaks were reached on days 21 and 27 post infection. The results of this study demonstrate that eosinophils occur abundantly in two transcendent moments of the <i>T. spiralis</i> life cycle: first, when the stage 1 larva invades the myocyte and second when the nurse cell-larva complex has been fully developed. These results help one to understand the immunobiology of <i>T. spiralis</i>, highlighting the importance of eosinophils in the survival of the larva in skeletal muscle. Further studies are needed to characterize the cell populations that comprise the cellular infiltrate during the development of the mother cell.Vicente Vega-SánchezFabián-Ricardo Gómez-De-AndaGeorgina Calderón-DomínguezMary-Carmen-del-Sol Ramírez-y-RamírezNydia-E. Reyes-RodríguezAndrea-P. Zepeda-VelázquezRaquel Tapia-RomeroJorge-Luis de-la-Rosa-AranaMDPI AGarticlecellular infiltrateeosinophilnurse cellexperimental infection<i>Trichinella spiralis</i>MedicineRENPathogens, Vol 10, Iss 1382, p 1382 (2021) |
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cellular infiltrate eosinophil nurse cell experimental infection <i>Trichinella spiralis</i> Medicine R |
| spellingShingle |
cellular infiltrate eosinophil nurse cell experimental infection <i>Trichinella spiralis</i> Medicine R Vicente Vega-Sánchez Fabián-Ricardo Gómez-De-Anda Georgina Calderón-Domínguez Mary-Carmen-del-Sol Ramírez-y-Ramírez Nydia-E. Reyes-Rodríguez Andrea-P. Zepeda-Velázquez Raquel Tapia-Romero Jorge-Luis de-la-Rosa-Arana Kinetics of Eosinophils during Development of the Cellular Infiltrate Surrounding the Nurse Cell of <i>Trichinella spiralis</i> in Experimentally Infected Mice |
| description |
We study the kinetics of eosinophils during the development of the cellular infiltrate surrounding the nurse cell of <i>Trichinella spiralis</i> (<i>T. spiralis</i>) in experimentally infected mice. Male CD1 mice were experimentally infected with 50 viable muscle larvae of the MSUS/MEX/91/CM-91 <i>T. spiralis</i> strain. Tongues and diaphragms were obtained daily from days 13 to 39 post infection. Diaphragms were compressed and subjected to Giemsa stain. Tongues were histologically sectioned and stained with erythrosine B or hematoxylin and eosin. The cellular infiltrate and the nurse cell-larva complex were detected by optical microscopy since day 16 post infection. The size of the larva increased exponentially during the course of the infection. The kinetics of eosinophils showed a multimodal trend, with a bimodal predominance. The maximum peaks were reached on days 21 and 27 post infection. The results of this study demonstrate that eosinophils occur abundantly in two transcendent moments of the <i>T. spiralis</i> life cycle: first, when the stage 1 larva invades the myocyte and second when the nurse cell-larva complex has been fully developed. These results help one to understand the immunobiology of <i>T. spiralis</i>, highlighting the importance of eosinophils in the survival of the larva in skeletal muscle. Further studies are needed to characterize the cell populations that comprise the cellular infiltrate during the development of the mother cell. |
| format |
article |
| author |
Vicente Vega-Sánchez Fabián-Ricardo Gómez-De-Anda Georgina Calderón-Domínguez Mary-Carmen-del-Sol Ramírez-y-Ramírez Nydia-E. Reyes-Rodríguez Andrea-P. Zepeda-Velázquez Raquel Tapia-Romero Jorge-Luis de-la-Rosa-Arana |
| author_facet |
Vicente Vega-Sánchez Fabián-Ricardo Gómez-De-Anda Georgina Calderón-Domínguez Mary-Carmen-del-Sol Ramírez-y-Ramírez Nydia-E. Reyes-Rodríguez Andrea-P. Zepeda-Velázquez Raquel Tapia-Romero Jorge-Luis de-la-Rosa-Arana |
| author_sort |
Vicente Vega-Sánchez |
| title |
Kinetics of Eosinophils during Development of the Cellular Infiltrate Surrounding the Nurse Cell of <i>Trichinella spiralis</i> in Experimentally Infected Mice |
| title_short |
Kinetics of Eosinophils during Development of the Cellular Infiltrate Surrounding the Nurse Cell of <i>Trichinella spiralis</i> in Experimentally Infected Mice |
| title_full |
Kinetics of Eosinophils during Development of the Cellular Infiltrate Surrounding the Nurse Cell of <i>Trichinella spiralis</i> in Experimentally Infected Mice |
| title_fullStr |
Kinetics of Eosinophils during Development of the Cellular Infiltrate Surrounding the Nurse Cell of <i>Trichinella spiralis</i> in Experimentally Infected Mice |
| title_full_unstemmed |
Kinetics of Eosinophils during Development of the Cellular Infiltrate Surrounding the Nurse Cell of <i>Trichinella spiralis</i> in Experimentally Infected Mice |
| title_sort |
kinetics of eosinophils during development of the cellular infiltrate surrounding the nurse cell of <i>trichinella spiralis</i> in experimentally infected mice |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/bd83b1e07d1645899293585b9959a5b1 |
| work_keys_str_mv |
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| _version_ |
1718410917866110976 |