A mammalian mirtron miR-1224 promotes tube-formation of human primary endothelial cells by targeting anti-angiogenic factor epsin2

Abstract Angiogenesis, new vessel formation from pre-existing vessels, is a highly conserved event through vertebrates. However, the system for tuning angiogenesis by species-intrinsic factors is totally unknown. miR-1224 is a member of mammal-specific mirtrons, which were identified as non-canonica...

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Autores principales:	Eiko Sakai, Yusuke Miura, Emi Suzuki-Kouyama, Kengo Oka, Masashi Tachibana, Kenji Kawabata, Fuminori Sakurai, Hiroyuki Mizuguchi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bd865baf34144ea8b49fb925209bcf32
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spelling	oai:doaj.org-article:bd865baf34144ea8b49fb925209bcf322021-12-02T16:06:29ZA mammalian mirtron miR-1224 promotes tube-formation of human primary endothelial cells by targeting anti-angiogenic factor epsin210.1038/s41598-017-05782-32045-2322https://doaj.org/article/bd865baf34144ea8b49fb925209bcf322017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05782-3https://doaj.org/toc/2045-2322Abstract Angiogenesis, new vessel formation from pre-existing vessels, is a highly conserved event through vertebrates. However, the system for tuning angiogenesis by species-intrinsic factors is totally unknown. miR-1224 is a member of mammal-specific mirtrons, which were identified as non-canonical microRNAs. We found that the expression of miR-1224 was upregulated in capillary-like tube-forming human umbilical vein endothelial cells on Matrigel. Enforced expression of miR-1224 stimulated tube formation, whereas repression of endogenous miR-1224 inhibited formation. Enforced expression of miR-1224 enhanced VEGF signaling and repressed NOTCH signaling. The adaptor protein of clathrin-dependent endocytosis, epsin2, which has been shown to be a suppressor of angiogenesis, was a direct target of miR-1224. Knockdown of EPN2 stimulated tube formation, while overexpression of EPN2 repressed miR-1224-mediated stimulation. Our findings indicate that miR-1224 is a mammal specific modulator of angiogenesis.Eiko SakaiYusuke MiuraEmi Suzuki-KouyamaKengo OkaMasashi TachibanaKenji KawabataFuminori SakuraiHiroyuki MizuguchiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eiko Sakai Yusuke Miura Emi Suzuki-Kouyama Kengo Oka Masashi Tachibana Kenji Kawabata Fuminori Sakurai Hiroyuki Mizuguchi A mammalian mirtron miR-1224 promotes tube-formation of human primary endothelial cells by targeting anti-angiogenic factor epsin2
description	Abstract Angiogenesis, new vessel formation from pre-existing vessels, is a highly conserved event through vertebrates. However, the system for tuning angiogenesis by species-intrinsic factors is totally unknown. miR-1224 is a member of mammal-specific mirtrons, which were identified as non-canonical microRNAs. We found that the expression of miR-1224 was upregulated in capillary-like tube-forming human umbilical vein endothelial cells on Matrigel. Enforced expression of miR-1224 stimulated tube formation, whereas repression of endogenous miR-1224 inhibited formation. Enforced expression of miR-1224 enhanced VEGF signaling and repressed NOTCH signaling. The adaptor protein of clathrin-dependent endocytosis, epsin2, which has been shown to be a suppressor of angiogenesis, was a direct target of miR-1224. Knockdown of EPN2 stimulated tube formation, while overexpression of EPN2 repressed miR-1224-mediated stimulation. Our findings indicate that miR-1224 is a mammal specific modulator of angiogenesis.
format	article
author	Eiko Sakai Yusuke Miura Emi Suzuki-Kouyama Kengo Oka Masashi Tachibana Kenji Kawabata Fuminori Sakurai Hiroyuki Mizuguchi
author_facet	Eiko Sakai Yusuke Miura Emi Suzuki-Kouyama Kengo Oka Masashi Tachibana Kenji Kawabata Fuminori Sakurai Hiroyuki Mizuguchi
author_sort	Eiko Sakai
title	A mammalian mirtron miR-1224 promotes tube-formation of human primary endothelial cells by targeting anti-angiogenic factor epsin2
title_short	A mammalian mirtron miR-1224 promotes tube-formation of human primary endothelial cells by targeting anti-angiogenic factor epsin2
title_full	A mammalian mirtron miR-1224 promotes tube-formation of human primary endothelial cells by targeting anti-angiogenic factor epsin2
title_fullStr	A mammalian mirtron miR-1224 promotes tube-formation of human primary endothelial cells by targeting anti-angiogenic factor epsin2
title_full_unstemmed	A mammalian mirtron miR-1224 promotes tube-formation of human primary endothelial cells by targeting anti-angiogenic factor epsin2
title_sort	mammalian mirtron mir-1224 promotes tube-formation of human primary endothelial cells by targeting anti-angiogenic factor epsin2
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bd865baf34144ea8b49fb925209bcf32
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A mammalian mirtron miR-1224 promotes tube-formation of human primary endothelial cells by targeting anti-angiogenic factor epsin2

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