Fluorescence-tagged transgenic lines reveal genetic defects in pollen growth--application to the eIF3 complex.

<h4>Background</h4>Mutations in several subunits of eukaryotic translation initiation factor 3 (eIF3) cause male transmission defects in Arabidopsis thaliana. To identify the stage of pollen development at which eIF3 becomes essential it is desirable to examine viable pollen and distingu...

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Autores principales:	Bijoyita Roy, Gregory P Copenhaver, Albrecht G von Arnim
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bd877f87df9f4b9e9d002edc9ce699de
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spelling	oai:doaj.org-article:bd877f87df9f4b9e9d002edc9ce699de2021-11-18T06:57:42ZFluorescence-tagged transgenic lines reveal genetic defects in pollen growth--application to the eIF3 complex.1932-620310.1371/journal.pone.0017640https://doaj.org/article/bd877f87df9f4b9e9d002edc9ce699de2011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21408229/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Mutations in several subunits of eukaryotic translation initiation factor 3 (eIF3) cause male transmission defects in Arabidopsis thaliana. To identify the stage of pollen development at which eIF3 becomes essential it is desirable to examine viable pollen and distinguish mutant from wild type. To accomplish this we have developed a broadly applicable method to track mutant alleles that are not already tagged by a visible marker gene through the male lineage of Arabidopsis.<h4>Methodology/principal findings</h4>Fluorescence tagged lines (FTLs) harbor a transgenic fluorescent protein gene (XFP) expressed by the pollen-specific LAT52 promoter at a defined chromosomal position. In the existing collection of FTLs there are enough XFP marker genes to track nearly every nuclear gene by virtue of its genetic linkage to a transgenic marker gene. Using FTLs in a quartet mutant, which yields mature pollen tetrads, we determined that the pollen transmission defect of the eif3h-1 allele is due to a combination of reduced pollen germination and reduced pollen tube elongation. We also detected reduced pollen germination for eif3e. However, neither eif3h nor eif3e, unlike other known gametophytic mutations, measurably disrupted the early stages of pollen maturation.<h4>Conclusion/significance</h4>eIF3h and eIF3e both become essential during pollen germination, a stage of vigorous translation of newly transcribed mRNAs. These data delimit the end of the developmental window during which paternal rescue is still possible. Moreover, the FTL collection of mapped fluorescent protein transgenes represents an attractive resource for elucidating the pollen development phenotypes of any fine-mapped mutation in Arabidopsis.Bijoyita RoyGregory P CopenhaverAlbrecht G von ArnimPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e17640 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bijoyita Roy Gregory P Copenhaver Albrecht G von Arnim Fluorescence-tagged transgenic lines reveal genetic defects in pollen growth--application to the eIF3 complex.
description	<h4>Background</h4>Mutations in several subunits of eukaryotic translation initiation factor 3 (eIF3) cause male transmission defects in Arabidopsis thaliana. To identify the stage of pollen development at which eIF3 becomes essential it is desirable to examine viable pollen and distinguish mutant from wild type. To accomplish this we have developed a broadly applicable method to track mutant alleles that are not already tagged by a visible marker gene through the male lineage of Arabidopsis.<h4>Methodology/principal findings</h4>Fluorescence tagged lines (FTLs) harbor a transgenic fluorescent protein gene (XFP) expressed by the pollen-specific LAT52 promoter at a defined chromosomal position. In the existing collection of FTLs there are enough XFP marker genes to track nearly every nuclear gene by virtue of its genetic linkage to a transgenic marker gene. Using FTLs in a quartet mutant, which yields mature pollen tetrads, we determined that the pollen transmission defect of the eif3h-1 allele is due to a combination of reduced pollen germination and reduced pollen tube elongation. We also detected reduced pollen germination for eif3e. However, neither eif3h nor eif3e, unlike other known gametophytic mutations, measurably disrupted the early stages of pollen maturation.<h4>Conclusion/significance</h4>eIF3h and eIF3e both become essential during pollen germination, a stage of vigorous translation of newly transcribed mRNAs. These data delimit the end of the developmental window during which paternal rescue is still possible. Moreover, the FTL collection of mapped fluorescent protein transgenes represents an attractive resource for elucidating the pollen development phenotypes of any fine-mapped mutation in Arabidopsis.
format	article
author	Bijoyita Roy Gregory P Copenhaver Albrecht G von Arnim
author_facet	Bijoyita Roy Gregory P Copenhaver Albrecht G von Arnim
author_sort	Bijoyita Roy
title	Fluorescence-tagged transgenic lines reveal genetic defects in pollen growth--application to the eIF3 complex.
title_short	Fluorescence-tagged transgenic lines reveal genetic defects in pollen growth--application to the eIF3 complex.
title_full	Fluorescence-tagged transgenic lines reveal genetic defects in pollen growth--application to the eIF3 complex.
title_fullStr	Fluorescence-tagged transgenic lines reveal genetic defects in pollen growth--application to the eIF3 complex.
title_full_unstemmed	Fluorescence-tagged transgenic lines reveal genetic defects in pollen growth--application to the eIF3 complex.
title_sort	fluorescence-tagged transgenic lines reveal genetic defects in pollen growth--application to the eif3 complex.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/bd877f87df9f4b9e9d002edc9ce699de
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Fluorescence-tagged transgenic lines reveal genetic defects in pollen growth--application to the eIF3 complex.

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