Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation

Throughput of in vivo genetic screens is a barrier to efficient application. Here the authors use a high-throughput CRISPR-based in vivo forward genetic screen in mice to identify transcriptional regulators of cardiomyocyte maturation, including the epigenetic modifiers RNF20 and RNF40.

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Autores principales:	Nathan J. VanDusen, Julianna Y. Lee, Weiliang Gu, Catalina E. Butler, Isha Sethi, Yanjiang Zheng, Justin S. King, Pingzhu Zhou, Shengbao Suo, Yuxuan Guo, Qing Ma, Guo-Cheng Yuan, William William Pu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/bd881c2a13fb42f1a6bfafcd640b11a4
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spelling	oai:doaj.org-article:bd881c2a13fb42f1a6bfafcd640b11a42021-12-02T17:57:04ZMassively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation10.1038/s41467-021-24743-z2041-1723https://doaj.org/article/bd881c2a13fb42f1a6bfafcd640b11a42021-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-24743-zhttps://doaj.org/toc/2041-1723Throughput of in vivo genetic screens is a barrier to efficient application. Here the authors use a high-throughput CRISPR-based in vivo forward genetic screen in mice to identify transcriptional regulators of cardiomyocyte maturation, including the epigenetic modifiers RNF20 and RNF40.Nathan J. VanDusenJulianna Y. LeeWeiliang GuCatalina E. ButlerIsha SethiYanjiang ZhengJustin S. KingPingzhu ZhouShengbao SuoYuxuan GuoQing MaGuo-Cheng YuanWilliam William PuNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Nathan J. VanDusen Julianna Y. Lee Weiliang Gu Catalina E. Butler Isha Sethi Yanjiang Zheng Justin S. King Pingzhu Zhou Shengbao Suo Yuxuan Guo Qing Ma Guo-Cheng Yuan William William Pu Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation
description	Throughput of in vivo genetic screens is a barrier to efficient application. Here the authors use a high-throughput CRISPR-based in vivo forward genetic screen in mice to identify transcriptional regulators of cardiomyocyte maturation, including the epigenetic modifiers RNF20 and RNF40.
format	article
author	Nathan J. VanDusen Julianna Y. Lee Weiliang Gu Catalina E. Butler Isha Sethi Yanjiang Zheng Justin S. King Pingzhu Zhou Shengbao Suo Yuxuan Guo Qing Ma Guo-Cheng Yuan William William Pu
author_facet	Nathan J. VanDusen Julianna Y. Lee Weiliang Gu Catalina E. Butler Isha Sethi Yanjiang Zheng Justin S. King Pingzhu Zhou Shengbao Suo Yuxuan Guo Qing Ma Guo-Cheng Yuan William William Pu
author_sort	Nathan J. VanDusen
title	Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation
title_short	Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation
title_full	Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation
title_fullStr	Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation
title_full_unstemmed	Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation
title_sort	massively parallel in vivo crispr screening identifies rnf20/40 as epigenetic regulators of cardiomyocyte maturation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bd881c2a13fb42f1a6bfafcd640b11a4
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Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation

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