Degradability, biocompatibility, and osteogenesis of biocomposite scaffolds containing nano magnesium phosphate and wheat protein both in vitro and in vivo for bone regeneration
Yan Xia,1 Panyu Zhou,1,* Fei Wang,2 Chao Qiu,2 Panfeng Wang,1 Yuntong Zhang,1 Liming Zhao,3 Shuogui Xu1,2 1Department of Emergency, 2Department of Orthopedics, Changhai Hospital, Second Military Medical University, 3State Key Laboratory of Bioreactor Engineering, East China University of Science an...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2016
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/bd8d82ca70084832adced3d40c861e30 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:bd8d82ca70084832adced3d40c861e30 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:bd8d82ca70084832adced3d40c861e302021-12-02T08:06:24ZDegradability, biocompatibility, and osteogenesis of biocomposite scaffolds containing nano magnesium phosphate and wheat protein both in vitro and in vivo for bone regeneration1178-2013https://doaj.org/article/bd8d82ca70084832adced3d40c861e302016-07-01T00:00:00Zhttps://www.dovepress.com/degradability--biocompatibility-and-osteogenesis-of-biocomposite-scaff-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yan Xia,1 Panyu Zhou,1,* Fei Wang,2 Chao Qiu,2 Panfeng Wang,1 Yuntong Zhang,1 Liming Zhao,3 Shuogui Xu1,2 1Department of Emergency, 2Department of Orthopedics, Changhai Hospital, Second Military Medical University, 3State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: In this study, bioactive scaffold of nano magnesium phosphate (nMP)/wheat protein (WP) composite (MWC) was fabricated. The results revealed that the MWC scaffolds had interconnected not only macropores (sized 400–600 µm) but also micropores (sized 10–20 µm) on the walls of macropores. The MWC scaffolds containing 40 w% nMP had an appropriate degradability in phosphate-buffered saline and produced a weak alkaline microenvironment. In cell culture experiments, the results revealed that the MWC scaffolds significantly promoted the MC3T3-E1 cell proliferation, differentiation, and growth into the scaffolds. The results of synchrotron radiation microcomputed tomography and analysis of the histological sections of the in vivo implantation revealed that the MWC scaffolds evidently improved the new bone formation and bone defects repair as compared with WP scaffolds. Moreover, it was found that newly formed bone tissue continued to increase with the gradual reduction of materials residual in the MWC scaffolds. Furthermore, the immunohistochemical analysis further offered the evidence of the stimulatory effects of MWC scaffolds on osteogenic-related cell differentiation and new bone regeneration. The results indicated that MWC scaffolds with good biocompability and degradability could promote osteogenesis in vivo, which would have potential for bone tissue repair. Keywords: nano magnesium phosphate, wheat protein, composite scaffolds, degradation, bone regenerationXia YZhou PWang FQiu CWang PZhang YZhao LXu SDove Medical PressarticleNano magnesium phosphatewheat proteincomposite scaffoldsdegradationbone regenerationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 3435-3449 (2016) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Nano magnesium phosphate wheat protein composite scaffolds degradation bone regeneration Medicine (General) R5-920 |
| spellingShingle |
Nano magnesium phosphate wheat protein composite scaffolds degradation bone regeneration Medicine (General) R5-920 Xia Y Zhou P Wang F Qiu C Wang P Zhang Y Zhao L Xu S Degradability, biocompatibility, and osteogenesis of biocomposite scaffolds containing nano magnesium phosphate and wheat protein both in vitro and in vivo for bone regeneration |
| description |
Yan Xia,1 Panyu Zhou,1,* Fei Wang,2 Chao Qiu,2 Panfeng Wang,1 Yuntong Zhang,1 Liming Zhao,3 Shuogui Xu1,2 1Department of Emergency, 2Department of Orthopedics, Changhai Hospital, Second Military Medical University, 3State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: In this study, bioactive scaffold of nano magnesium phosphate (nMP)/wheat protein (WP) composite (MWC) was fabricated. The results revealed that the MWC scaffolds had interconnected not only macropores (sized 400–600 µm) but also micropores (sized 10–20 µm) on the walls of macropores. The MWC scaffolds containing 40 w% nMP had an appropriate degradability in phosphate-buffered saline and produced a weak alkaline microenvironment. In cell culture experiments, the results revealed that the MWC scaffolds significantly promoted the MC3T3-E1 cell proliferation, differentiation, and growth into the scaffolds. The results of synchrotron radiation microcomputed tomography and analysis of the histological sections of the in vivo implantation revealed that the MWC scaffolds evidently improved the new bone formation and bone defects repair as compared with WP scaffolds. Moreover, it was found that newly formed bone tissue continued to increase with the gradual reduction of materials residual in the MWC scaffolds. Furthermore, the immunohistochemical analysis further offered the evidence of the stimulatory effects of MWC scaffolds on osteogenic-related cell differentiation and new bone regeneration. The results indicated that MWC scaffolds with good biocompability and degradability could promote osteogenesis in vivo, which would have potential for bone tissue repair. Keywords: nano magnesium phosphate, wheat protein, composite scaffolds, degradation, bone regeneration |
| format |
article |
| author |
Xia Y Zhou P Wang F Qiu C Wang P Zhang Y Zhao L Xu S |
| author_facet |
Xia Y Zhou P Wang F Qiu C Wang P Zhang Y Zhao L Xu S |
| author_sort |
Xia Y |
| title |
Degradability, biocompatibility, and osteogenesis of biocomposite scaffolds containing nano magnesium phosphate and wheat protein both in vitro and in vivo for bone regeneration |
| title_short |
Degradability, biocompatibility, and osteogenesis of biocomposite scaffolds containing nano magnesium phosphate and wheat protein both in vitro and in vivo for bone regeneration |
| title_full |
Degradability, biocompatibility, and osteogenesis of biocomposite scaffolds containing nano magnesium phosphate and wheat protein both in vitro and in vivo for bone regeneration |
| title_fullStr |
Degradability, biocompatibility, and osteogenesis of biocomposite scaffolds containing nano magnesium phosphate and wheat protein both in vitro and in vivo for bone regeneration |
| title_full_unstemmed |
Degradability, biocompatibility, and osteogenesis of biocomposite scaffolds containing nano magnesium phosphate and wheat protein both in vitro and in vivo for bone regeneration |
| title_sort |
degradability, biocompatibility, and osteogenesis of biocomposite scaffolds containing nano magnesium phosphate and wheat protein both in vitro and in vivo for bone regeneration |
| publisher |
Dove Medical Press |
| publishDate |
2016 |
| url |
https://doaj.org/article/bd8d82ca70084832adced3d40c861e30 |
| work_keys_str_mv |
AT xiay degradabilitybiocompatibilityandosteogenesisofbiocompositescaffoldscontainingnanomagnesiumphosphateandwheatproteinbothinvitroandinvivoforboneregeneration AT zhoup degradabilitybiocompatibilityandosteogenesisofbiocompositescaffoldscontainingnanomagnesiumphosphateandwheatproteinbothinvitroandinvivoforboneregeneration AT wangf degradabilitybiocompatibilityandosteogenesisofbiocompositescaffoldscontainingnanomagnesiumphosphateandwheatproteinbothinvitroandinvivoforboneregeneration AT qiuc degradabilitybiocompatibilityandosteogenesisofbiocompositescaffoldscontainingnanomagnesiumphosphateandwheatproteinbothinvitroandinvivoforboneregeneration AT wangp degradabilitybiocompatibilityandosteogenesisofbiocompositescaffoldscontainingnanomagnesiumphosphateandwheatproteinbothinvitroandinvivoforboneregeneration AT zhangy degradabilitybiocompatibilityandosteogenesisofbiocompositescaffoldscontainingnanomagnesiumphosphateandwheatproteinbothinvitroandinvivoforboneregeneration AT zhaol degradabilitybiocompatibilityandosteogenesisofbiocompositescaffoldscontainingnanomagnesiumphosphateandwheatproteinbothinvitroandinvivoforboneregeneration AT xus degradabilitybiocompatibilityandosteogenesisofbiocompositescaffoldscontainingnanomagnesiumphosphateandwheatproteinbothinvitroandinvivoforboneregeneration |
| _version_ |
1718398685336829952 |