Long-term memory requires sequential protein synthesis in three subsets of mushroom body output neurons in Drosophila

Abstract Creating long-term memory (LTM) requires new protein synthesis to stabilize learning-induced synaptic changes in the brain. In the fruit fly, Drosophila melanogaster, aversive olfactory learning forms several phases of labile memory to associate an odor with coincident punishment in the mus...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jie-Kai Wu, Chu-Yi Tai, Kuan-Lin Feng, Shiu-Ling Chen, Chun-Chao Chen, Ann-Shyn Chiang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bd8fafb0abad4cc3b8f568065537eadb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bd8fafb0abad4cc3b8f568065537eadb
record_format	dspace
spelling	oai:doaj.org-article:bd8fafb0abad4cc3b8f568065537eadb2021-12-02T15:05:21ZLong-term memory requires sequential protein synthesis in three subsets of mushroom body output neurons in Drosophila10.1038/s41598-017-07600-22045-2322https://doaj.org/article/bd8fafb0abad4cc3b8f568065537eadb2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07600-2https://doaj.org/toc/2045-2322Abstract Creating long-term memory (LTM) requires new protein synthesis to stabilize learning-induced synaptic changes in the brain. In the fruit fly, Drosophila melanogaster, aversive olfactory learning forms several phases of labile memory to associate an odor with coincident punishment in the mushroom body (MB). It remains unclear how the brain consolidates early labile memory into LTM. Here, we survey 183 Gal4 lines containing almost all 21 distinct types of MB output neurons (MBONs) and show that sequential synthesis of learning-induced proteins occurs at three types of MBONs. Downregulation of oo18 RNA-binding proteins (ORBs) in any of these MBONs impaired LTM. And, neurotransmission outputs from these MBONs are all required during LTM retrieval. Together, these results suggest an LTM consolidation model in which transient neural activities of early labile memory in the MB are consolidated into stable LTM at a few postsynaptic MBONs through sequential ORB-regulated local protein synthesis.Jie-Kai WuChu-Yi TaiKuan-Lin FengShiu-Ling ChenChun-Chao ChenAnn-Shyn ChiangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jie-Kai Wu Chu-Yi Tai Kuan-Lin Feng Shiu-Ling Chen Chun-Chao Chen Ann-Shyn Chiang Long-term memory requires sequential protein synthesis in three subsets of mushroom body output neurons in Drosophila
description	Abstract Creating long-term memory (LTM) requires new protein synthesis to stabilize learning-induced synaptic changes in the brain. In the fruit fly, Drosophila melanogaster, aversive olfactory learning forms several phases of labile memory to associate an odor with coincident punishment in the mushroom body (MB). It remains unclear how the brain consolidates early labile memory into LTM. Here, we survey 183 Gal4 lines containing almost all 21 distinct types of MB output neurons (MBONs) and show that sequential synthesis of learning-induced proteins occurs at three types of MBONs. Downregulation of oo18 RNA-binding proteins (ORBs) in any of these MBONs impaired LTM. And, neurotransmission outputs from these MBONs are all required during LTM retrieval. Together, these results suggest an LTM consolidation model in which transient neural activities of early labile memory in the MB are consolidated into stable LTM at a few postsynaptic MBONs through sequential ORB-regulated local protein synthesis.
format	article
author	Jie-Kai Wu Chu-Yi Tai Kuan-Lin Feng Shiu-Ling Chen Chun-Chao Chen Ann-Shyn Chiang
author_facet	Jie-Kai Wu Chu-Yi Tai Kuan-Lin Feng Shiu-Ling Chen Chun-Chao Chen Ann-Shyn Chiang
author_sort	Jie-Kai Wu
title	Long-term memory requires sequential protein synthesis in three subsets of mushroom body output neurons in Drosophila
title_short	Long-term memory requires sequential protein synthesis in three subsets of mushroom body output neurons in Drosophila
title_full	Long-term memory requires sequential protein synthesis in three subsets of mushroom body output neurons in Drosophila
title_fullStr	Long-term memory requires sequential protein synthesis in three subsets of mushroom body output neurons in Drosophila
title_full_unstemmed	Long-term memory requires sequential protein synthesis in three subsets of mushroom body output neurons in Drosophila
title_sort	long-term memory requires sequential protein synthesis in three subsets of mushroom body output neurons in drosophila
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bd8fafb0abad4cc3b8f568065537eadb
work_keys_str_mv	AT jiekaiwu longtermmemoryrequiressequentialproteinsynthesisinthreesubsetsofmushroombodyoutputneuronsindrosophila AT chuyitai longtermmemoryrequiressequentialproteinsynthesisinthreesubsetsofmushroombodyoutputneuronsindrosophila AT kuanlinfeng longtermmemoryrequiressequentialproteinsynthesisinthreesubsetsofmushroombodyoutputneuronsindrosophila AT shiulingchen longtermmemoryrequiressequentialproteinsynthesisinthreesubsetsofmushroombodyoutputneuronsindrosophila AT chunchaochen longtermmemoryrequiressequentialproteinsynthesisinthreesubsetsofmushroombodyoutputneuronsindrosophila AT annshynchiang longtermmemoryrequiressequentialproteinsynthesisinthreesubsetsofmushroombodyoutputneuronsindrosophila
_version_	1718388854820438016

Long-term memory requires sequential protein synthesis in three subsets of mushroom body output neurons in Drosophila

Ejemplares similares