Expression of multiple artificial microRNAs from a chicken miRNA126-based lentiviral vector.

<h4>Background</h4>The use of RNAi in both basic and translational research often requires expression of multiple siRNAs from the same vector.<h4>Methods/principal findings</h4>We have developed a novel chicken miR126-based artificial miRNA expression system that can express...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Steve C-Y Chen, Patrick Stern, Zhuyan Guo, Jianzhu Chen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bd9302d4eb3c44a78ba84adf7a318e03
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bd9302d4eb3c44a78ba84adf7a318e03
record_format	dspace
spelling	oai:doaj.org-article:bd9302d4eb3c44a78ba84adf7a318e032021-11-18T06:50:04ZExpression of multiple artificial microRNAs from a chicken miRNA126-based lentiviral vector.1932-620310.1371/journal.pone.0022437https://doaj.org/article/bd9302d4eb3c44a78ba84adf7a318e032011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21789258/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The use of RNAi in both basic and translational research often requires expression of multiple siRNAs from the same vector.<h4>Methods/principal findings</h4>We have developed a novel chicken miR126-based artificial miRNA expression system that can express one, two or three miRNAs from a single cassette in a lentiviral vector. We show that each of the miRNAs expressed from the same lentiviral vector is capable of potent inhibition of reporter gene expression in transient transfection and stable integration assays in chicken fibroblast DF-1 cells. Transduction of Vero cells with lentivirus expressing two or three different anti-influenza miRNAs leads to inhibition of influenza virus production. In addition, the chicken miR126-based expression system effectively inhibits reporter gene expression in human, monkey, dog and mouse cells. These results demonstrate that the flanking regions of a single primary miRNA can support processing of three different stem-loops in a single vector.<h4>Conclusions/significance</h4>This novel design expands the means to express multiple miRNAs from the same vector for potent and effective silencing of target genes and influenza virus.Steve C-Y ChenPatrick SternZhuyan GuoJianzhu ChenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22437 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Steve C-Y Chen Patrick Stern Zhuyan Guo Jianzhu Chen Expression of multiple artificial microRNAs from a chicken miRNA126-based lentiviral vector.
description	<h4>Background</h4>The use of RNAi in both basic and translational research often requires expression of multiple siRNAs from the same vector.<h4>Methods/principal findings</h4>We have developed a novel chicken miR126-based artificial miRNA expression system that can express one, two or three miRNAs from a single cassette in a lentiviral vector. We show that each of the miRNAs expressed from the same lentiviral vector is capable of potent inhibition of reporter gene expression in transient transfection and stable integration assays in chicken fibroblast DF-1 cells. Transduction of Vero cells with lentivirus expressing two or three different anti-influenza miRNAs leads to inhibition of influenza virus production. In addition, the chicken miR126-based expression system effectively inhibits reporter gene expression in human, monkey, dog and mouse cells. These results demonstrate that the flanking regions of a single primary miRNA can support processing of three different stem-loops in a single vector.<h4>Conclusions/significance</h4>This novel design expands the means to express multiple miRNAs from the same vector for potent and effective silencing of target genes and influenza virus.
format	article
author	Steve C-Y Chen Patrick Stern Zhuyan Guo Jianzhu Chen
author_facet	Steve C-Y Chen Patrick Stern Zhuyan Guo Jianzhu Chen
author_sort	Steve C-Y Chen
title	Expression of multiple artificial microRNAs from a chicken miRNA126-based lentiviral vector.
title_short	Expression of multiple artificial microRNAs from a chicken miRNA126-based lentiviral vector.
title_full	Expression of multiple artificial microRNAs from a chicken miRNA126-based lentiviral vector.
title_fullStr	Expression of multiple artificial microRNAs from a chicken miRNA126-based lentiviral vector.
title_full_unstemmed	Expression of multiple artificial microRNAs from a chicken miRNA126-based lentiviral vector.
title_sort	expression of multiple artificial micrornas from a chicken mirna126-based lentiviral vector.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/bd9302d4eb3c44a78ba84adf7a318e03
work_keys_str_mv	AT stevecychen expressionofmultipleartificialmicrornasfromachickenmirna126basedlentiviralvector AT patrickstern expressionofmultipleartificialmicrornasfromachickenmirna126basedlentiviralvector AT zhuyanguo expressionofmultipleartificialmicrornasfromachickenmirna126basedlentiviralvector AT jianzhuchen expressionofmultipleartificialmicrornasfromachickenmirna126basedlentiviralvector
_version_	1718424316858597376

Expression of multiple artificial microRNAs from a chicken miRNA126-based lentiviral vector.

Ejemplares similares