Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons.

Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this...

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Autores principales:	Luísa Pinto, António Mateus-Pinheiro, Mónica Morais, João Miguel Bessa, Nuno Sousa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bd9327759be54dccae0e622a69c30e3c
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spelling	oai:doaj.org-article:bd9327759be54dccae0e622a69c30e3c2021-11-18T07:25:27ZImmuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons.1932-620310.1371/journal.pone.0033114https://doaj.org/article/bd9327759be54dccae0e622a69c30e3c2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22427964/?tool=EBIhttps://doaj.org/toc/1932-6203Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this simple non-invasive method, we are able to determine the tridimensional structure of dendritic arborization and spine shape of neurons born at a specific time in the hippocampus of adult animals. This analysis is relevant in physiological and pathological conditions in which altered neurogenesis is implicated, such as aging or emotional disorders.Luísa PintoAntónio Mateus-PinheiroMónica MoraisJoão Miguel BessaNuno SousaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e33114 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luísa Pinto António Mateus-Pinheiro Mónica Morais João Miguel Bessa Nuno Sousa Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons.
description	Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this simple non-invasive method, we are able to determine the tridimensional structure of dendritic arborization and spine shape of neurons born at a specific time in the hippocampus of adult animals. This analysis is relevant in physiological and pathological conditions in which altered neurogenesis is implicated, such as aging or emotional disorders.
format	article
author	Luísa Pinto António Mateus-Pinheiro Mónica Morais João Miguel Bessa Nuno Sousa
author_facet	Luísa Pinto António Mateus-Pinheiro Mónica Morais João Miguel Bessa Nuno Sousa
author_sort	Luísa Pinto
title	Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons.
title_short	Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons.
title_full	Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons.
title_fullStr	Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons.
title_full_unstemmed	Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons.
title_sort	immuno-golgi as a tool for analyzing neuronal 3d-dendritic structure in phenotypically characterized neurons.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/bd9327759be54dccae0e622a69c30e3c
work_keys_str_mv	AT luisapinto immunogolgiasatoolforanalyzingneuronal3ddendriticstructureinphenotypicallycharacterizedneurons AT antoniomateuspinheiro immunogolgiasatoolforanalyzingneuronal3ddendriticstructureinphenotypicallycharacterizedneurons AT monicamorais immunogolgiasatoolforanalyzingneuronal3ddendriticstructureinphenotypicallycharacterizedneurons AT joaomiguelbessa immunogolgiasatoolforanalyzingneuronal3ddendriticstructureinphenotypicallycharacterizedneurons AT nunosousa immunogolgiasatoolforanalyzingneuronal3ddendriticstructureinphenotypicallycharacterizedneurons
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Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons.

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