Patterns in Wetland Microbial Community Composition and Functional Gene Repertoire Associated with Methane Emissions

ABSTRACT Wetland restoration on peat islands previously drained for agriculture has potential to reverse land subsidence and sequester atmospheric carbon dioxide as peat accretes. However, the emission of methane could potentially offset the greenhouse gas benefits of captured carbon. As microbial c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shaomei He, Stephanie A. Malfatti, Jack W. McFarland, Frank E. Anderson, Amrita Pati, Marcel Huntemann, Julien Tremblay, Tijana Glavina del Rio, Mark P. Waldrop, Lisamarie Windham-Myers, Susannah G. Tringe
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2015
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/bd95650dea7f47d9a6420070f344a25e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bd95650dea7f47d9a6420070f344a25e
record_format	dspace
spelling	oai:doaj.org-article:bd95650dea7f47d9a6420070f344a25e2021-11-15T15:49:02ZPatterns in Wetland Microbial Community Composition and Functional Gene Repertoire Associated with Methane Emissions10.1128/mBio.00066-152150-7511https://doaj.org/article/bd95650dea7f47d9a6420070f344a25e2015-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00066-15https://doaj.org/toc/2150-7511ABSTRACT Wetland restoration on peat islands previously drained for agriculture has potential to reverse land subsidence and sequester atmospheric carbon dioxide as peat accretes. However, the emission of methane could potentially offset the greenhouse gas benefits of captured carbon. As microbial communities play a key role in governing wetland greenhouse gas fluxes, we are interested in how microbial community composition and functions are associated with wetland hydrology, biogeochemistry, and methane emission, which is critical to modeling the microbial component in wetland methane fluxes and to managing restoration projects for maximal carbon sequestration. Here, we couple sequence-based methods with biogeochemical and greenhouse gas measurements to interrogate microbial communities from a pilot-scale restored wetland in the Sacramento-San Joaquin Delta of California, revealing considerable spatial heterogeneity even within this relatively small site. A number of microbial populations and functions showed strong correlations with electron acceptor availability and methane production; some also showed a preference for association with plant roots. Marker gene phylogenies revealed a diversity of major methane-producing and -consuming populations and suggested novel diversity within methanotrophs. Methanogenic archaea were observed in all samples, as were nitrate-, sulfate-, and metal-reducing bacteria, indicating that no single terminal electron acceptor was preferred despite differences in energetic favorability and suggesting spatial microheterogeneity and microniches. Notably, methanogens were negatively correlated with nitrate-, sulfate-, and metal-reducing bacteria and were most abundant at sampling sites with high peat accretion and low electron acceptor availability, where methane production was highest. IMPORTANCE Wetlands are the largest nonanthropogenic source of atmospheric methane but also a key global carbon reservoir. Characterizing belowground microbial communities that mediate carbon cycling in wetlands is critical to accurately predicting their responses to changes in land management and climate. Here, we studied a restored wetland and revealed substantial spatial heterogeneity in biogeochemistry, methane production, and microbial communities, largely associated with the wetland hydraulic design. We observed patterns in microbial community composition and functions correlated with biogeochemistry and methane production, including diverse microorganisms involved in methane production and consumption. We found that methanogenesis gene abundance is inversely correlated with genes from pathways exploiting other electron acceptors, yet the ubiquitous presence of genes from all these pathways suggests that diverse electron acceptors contribute to the energetic balance of the ecosystem. These investigations represent an important step toward effective management of wetlands to reduce methane flux to the atmosphere and enhance belowground carbon storage.Shaomei HeStephanie A. MalfattiJack W. McFarlandFrank E. AndersonAmrita PatiMarcel HuntemannJulien TremblayTijana Glavina del RioMark P. WaldropLisamarie Windham-MyersSusannah G. TringeAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 6, Iss 3 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Shaomei He Stephanie A. Malfatti Jack W. McFarland Frank E. Anderson Amrita Pati Marcel Huntemann Julien Tremblay Tijana Glavina del Rio Mark P. Waldrop Lisamarie Windham-Myers Susannah G. Tringe Patterns in Wetland Microbial Community Composition and Functional Gene Repertoire Associated with Methane Emissions
description	ABSTRACT Wetland restoration on peat islands previously drained for agriculture has potential to reverse land subsidence and sequester atmospheric carbon dioxide as peat accretes. However, the emission of methane could potentially offset the greenhouse gas benefits of captured carbon. As microbial communities play a key role in governing wetland greenhouse gas fluxes, we are interested in how microbial community composition and functions are associated with wetland hydrology, biogeochemistry, and methane emission, which is critical to modeling the microbial component in wetland methane fluxes and to managing restoration projects for maximal carbon sequestration. Here, we couple sequence-based methods with biogeochemical and greenhouse gas measurements to interrogate microbial communities from a pilot-scale restored wetland in the Sacramento-San Joaquin Delta of California, revealing considerable spatial heterogeneity even within this relatively small site. A number of microbial populations and functions showed strong correlations with electron acceptor availability and methane production; some also showed a preference for association with plant roots. Marker gene phylogenies revealed a diversity of major methane-producing and -consuming populations and suggested novel diversity within methanotrophs. Methanogenic archaea were observed in all samples, as were nitrate-, sulfate-, and metal-reducing bacteria, indicating that no single terminal electron acceptor was preferred despite differences in energetic favorability and suggesting spatial microheterogeneity and microniches. Notably, methanogens were negatively correlated with nitrate-, sulfate-, and metal-reducing bacteria and were most abundant at sampling sites with high peat accretion and low electron acceptor availability, where methane production was highest. IMPORTANCE Wetlands are the largest nonanthropogenic source of atmospheric methane but also a key global carbon reservoir. Characterizing belowground microbial communities that mediate carbon cycling in wetlands is critical to accurately predicting their responses to changes in land management and climate. Here, we studied a restored wetland and revealed substantial spatial heterogeneity in biogeochemistry, methane production, and microbial communities, largely associated with the wetland hydraulic design. We observed patterns in microbial community composition and functions correlated with biogeochemistry and methane production, including diverse microorganisms involved in methane production and consumption. We found that methanogenesis gene abundance is inversely correlated with genes from pathways exploiting other electron acceptors, yet the ubiquitous presence of genes from all these pathways suggests that diverse electron acceptors contribute to the energetic balance of the ecosystem. These investigations represent an important step toward effective management of wetlands to reduce methane flux to the atmosphere and enhance belowground carbon storage.
format	article
author	Shaomei He Stephanie A. Malfatti Jack W. McFarland Frank E. Anderson Amrita Pati Marcel Huntemann Julien Tremblay Tijana Glavina del Rio Mark P. Waldrop Lisamarie Windham-Myers Susannah G. Tringe
author_facet	Shaomei He Stephanie A. Malfatti Jack W. McFarland Frank E. Anderson Amrita Pati Marcel Huntemann Julien Tremblay Tijana Glavina del Rio Mark P. Waldrop Lisamarie Windham-Myers Susannah G. Tringe
author_sort	Shaomei He
title	Patterns in Wetland Microbial Community Composition and Functional Gene Repertoire Associated with Methane Emissions
title_short	Patterns in Wetland Microbial Community Composition and Functional Gene Repertoire Associated with Methane Emissions
title_full	Patterns in Wetland Microbial Community Composition and Functional Gene Repertoire Associated with Methane Emissions
title_fullStr	Patterns in Wetland Microbial Community Composition and Functional Gene Repertoire Associated with Methane Emissions
title_full_unstemmed	Patterns in Wetland Microbial Community Composition and Functional Gene Repertoire Associated with Methane Emissions
title_sort	patterns in wetland microbial community composition and functional gene repertoire associated with methane emissions
publisher	American Society for Microbiology
publishDate	2015
url	https://doaj.org/article/bd95650dea7f47d9a6420070f344a25e
work_keys_str_mv	AT shaomeihe patternsinwetlandmicrobialcommunitycompositionandfunctionalgenerepertoireassociatedwithmethaneemissions AT stephanieamalfatti patternsinwetlandmicrobialcommunitycompositionandfunctionalgenerepertoireassociatedwithmethaneemissions AT jackwmcfarland patternsinwetlandmicrobialcommunitycompositionandfunctionalgenerepertoireassociatedwithmethaneemissions AT frankeanderson patternsinwetlandmicrobialcommunitycompositionandfunctionalgenerepertoireassociatedwithmethaneemissions AT amritapati patternsinwetlandmicrobialcommunitycompositionandfunctionalgenerepertoireassociatedwithmethaneemissions AT marcelhuntemann patternsinwetlandmicrobialcommunitycompositionandfunctionalgenerepertoireassociatedwithmethaneemissions AT julientremblay patternsinwetlandmicrobialcommunitycompositionandfunctionalgenerepertoireassociatedwithmethaneemissions AT tijanaglavinadelrio patternsinwetlandmicrobialcommunitycompositionandfunctionalgenerepertoireassociatedwithmethaneemissions AT markpwaldrop patternsinwetlandmicrobialcommunitycompositionandfunctionalgenerepertoireassociatedwithmethaneemissions AT lisamariewindhammyers patternsinwetlandmicrobialcommunitycompositionandfunctionalgenerepertoireassociatedwithmethaneemissions AT susannahgtringe patternsinwetlandmicrobialcommunitycompositionandfunctionalgenerepertoireassociatedwithmethaneemissions
_version_	1718427545481773056

Patterns in Wetland Microbial Community Composition and Functional Gene Repertoire Associated with Methane Emissions

Ejemplares similares