Functional genomics of 5- to 8-cell stage human embryos by blastomere single-cell cDNA analysis.

Blastomere fate and embryonic genome activation (EGA) during human embryonic development are unsolved areas of high scientific and clinical interest. Forty-nine blastomeres from 5- to 8-cell human embryos have been investigated following an efficient single-cell cDNA amplification protocol to provid...

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Autores principales:	Amparo Galán, David Montaner, M Eugenia Póo, Diana Valbuena, Verónica Ruiz, Cristóbal Aguilar, Joaquín Dopazo, Carlos Simón
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bd9afea0b8694ed99087fc3cec4fb6a0
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spelling	oai:doaj.org-article:bd9afea0b8694ed99087fc3cec4fb6a02021-11-18T07:02:52ZFunctional genomics of 5- to 8-cell stage human embryos by blastomere single-cell cDNA analysis.1932-620310.1371/journal.pone.0013615https://doaj.org/article/bd9afea0b8694ed99087fc3cec4fb6a02010-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21049019/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Blastomere fate and embryonic genome activation (EGA) during human embryonic development are unsolved areas of high scientific and clinical interest. Forty-nine blastomeres from 5- to 8-cell human embryos have been investigated following an efficient single-cell cDNA amplification protocol to provide a template for high-density microarray analysis. The previously described markers, characteristic of Inner Cell Mass (ICM) (n = 120), stemness (n = 190) and Trophectoderm (TE) (n = 45), were analyzed, and a housekeeping pattern of 46 genes was established. All the human blastomeres from the 5- to 8-cell stage embryo displayed a common gene expression pattern corresponding to ICM markers (e.g., DDX3, FOXD3, LEFTY1, MYC, NANOG, POU5F1), stemness (e.g., POU5F1, DNMT3B, GABRB3, SOX2, ZFP42, TERT), and TE markers (e.g., GATA6, EOMES, CDX2, LHCGR). The EGA profile was also investigated between the 5-6- and 8-cell stage embryos, and compared to the blastocyst stage. Known genes (n = 92) such as depleted maternal transcripts (e.g., CCNA1, CCNB1, DPPA2) and embryo-specific activation (e.g., POU5F1, CDH1, DPPA4), as well as novel genes, were confirmed. In summary, the global single-cell cDNA amplification microarray analysis of the 5- to 8-cell stage human embryos reveals that blastomere fate is not committed to ICM or TE. Finally, new EGA features in human embryogenesis are presented.Amparo GalánDavid MontanerM Eugenia PóoDiana ValbuenaVerónica RuizCristóbal AguilarJoaquín DopazoCarlos SimónPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 10, p e13615 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amparo Galán David Montaner M Eugenia Póo Diana Valbuena Verónica Ruiz Cristóbal Aguilar Joaquín Dopazo Carlos Simón Functional genomics of 5- to 8-cell stage human embryos by blastomere single-cell cDNA analysis.
description	Blastomere fate and embryonic genome activation (EGA) during human embryonic development are unsolved areas of high scientific and clinical interest. Forty-nine blastomeres from 5- to 8-cell human embryos have been investigated following an efficient single-cell cDNA amplification protocol to provide a template for high-density microarray analysis. The previously described markers, characteristic of Inner Cell Mass (ICM) (n = 120), stemness (n = 190) and Trophectoderm (TE) (n = 45), were analyzed, and a housekeeping pattern of 46 genes was established. All the human blastomeres from the 5- to 8-cell stage embryo displayed a common gene expression pattern corresponding to ICM markers (e.g., DDX3, FOXD3, LEFTY1, MYC, NANOG, POU5F1), stemness (e.g., POU5F1, DNMT3B, GABRB3, SOX2, ZFP42, TERT), and TE markers (e.g., GATA6, EOMES, CDX2, LHCGR). The EGA profile was also investigated between the 5-6- and 8-cell stage embryos, and compared to the blastocyst stage. Known genes (n = 92) such as depleted maternal transcripts (e.g., CCNA1, CCNB1, DPPA2) and embryo-specific activation (e.g., POU5F1, CDH1, DPPA4), as well as novel genes, were confirmed. In summary, the global single-cell cDNA amplification microarray analysis of the 5- to 8-cell stage human embryos reveals that blastomere fate is not committed to ICM or TE. Finally, new EGA features in human embryogenesis are presented.
format	article
author	Amparo Galán David Montaner M Eugenia Póo Diana Valbuena Verónica Ruiz Cristóbal Aguilar Joaquín Dopazo Carlos Simón
author_facet	Amparo Galán David Montaner M Eugenia Póo Diana Valbuena Verónica Ruiz Cristóbal Aguilar Joaquín Dopazo Carlos Simón
author_sort	Amparo Galán
title	Functional genomics of 5- to 8-cell stage human embryos by blastomere single-cell cDNA analysis.
title_short	Functional genomics of 5- to 8-cell stage human embryos by blastomere single-cell cDNA analysis.
title_full	Functional genomics of 5- to 8-cell stage human embryos by blastomere single-cell cDNA analysis.
title_fullStr	Functional genomics of 5- to 8-cell stage human embryos by blastomere single-cell cDNA analysis.
title_full_unstemmed	Functional genomics of 5- to 8-cell stage human embryos by blastomere single-cell cDNA analysis.
title_sort	functional genomics of 5- to 8-cell stage human embryos by blastomere single-cell cdna analysis.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/bd9afea0b8694ed99087fc3cec4fb6a0
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Functional genomics of 5- to 8-cell stage human embryos by blastomere single-cell cDNA analysis.

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