Two-Phase Biocatalysis in Microfluidic Droplets

This Perspective discusses the literature related to two-phase biocatalysis in microfluidic droplets. Enzymes used as catalysts in biocatalysis are generally less stable in organic media than in their native aqueous environments; however, chemical and pharmaceutical compounds are often insoluble in...

Description complète

Enregistré dans:

Détails bibliographiques
Auteurs principaux:	Lanting Xiang, Felix Kaspar, Anett Schallmey, Iordania Constantinou
Format:	article
Langue:	EN
Publié:	MDPI AG 2021
Sujets:	microfluidics two-phase biocatalysis microfluidic droplets enzyme Biotechnology TP248.13-248.65
Accès en ligne:	https://doaj.org/article/bd9d502cd34142598f450fd924f6569a
Tags:	Ajouter un tag Pas de tags, Soyez le premier à ajouter un tag!

id	oai:doaj.org-article:bd9d502cd34142598f450fd924f6569a
record_format	dspace
spelling	oai:doaj.org-article:bd9d502cd34142598f450fd924f6569a2021-11-25T16:54:48ZTwo-Phase Biocatalysis in Microfluidic Droplets10.3390/bios111104072079-6374https://doaj.org/article/bd9d502cd34142598f450fd924f6569a2021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6374/11/11/407https://doaj.org/toc/2079-6374This Perspective discusses the literature related to two-phase biocatalysis in microfluidic droplets. Enzymes used as catalysts in biocatalysis are generally less stable in organic media than in their native aqueous environments; however, chemical and pharmaceutical compounds are often insoluble in water. The use of aqueous/organic two-phase media provides a solution to this problem and has therefore become standard practice for multiple biotransformations. In batch, two-phase biocatalysis is limited by mass transport, a limitation that can be overcome with the use of microfluidic systems. Although, two-phase biocatalysis in laminar flow systems has been extensively studied, microfluidic droplets have been primarily used for enzyme screening. In this Perspective, we summarize the limited published work on two-phase biocatalysis in microfluidic droplets and discuss the limitations, challenges, and future perspectives of this technology.Lanting XiangFelix KasparAnett SchallmeyIordania ConstantinouMDPI AGarticlemicrofluidicstwo-phase biocatalysismicrofluidic dropletsenzymeBiotechnologyTP248.13-248.65ENBiosensors, Vol 11, Iss 407, p 407 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microfluidics two-phase biocatalysis microfluidic droplets enzyme Biotechnology TP248.13-248.65
spellingShingle	microfluidics two-phase biocatalysis microfluidic droplets enzyme Biotechnology TP248.13-248.65 Lanting Xiang Felix Kaspar Anett Schallmey Iordania Constantinou Two-Phase Biocatalysis in Microfluidic Droplets
description	This Perspective discusses the literature related to two-phase biocatalysis in microfluidic droplets. Enzymes used as catalysts in biocatalysis are generally less stable in organic media than in their native aqueous environments; however, chemical and pharmaceutical compounds are often insoluble in water. The use of aqueous/organic two-phase media provides a solution to this problem and has therefore become standard practice for multiple biotransformations. In batch, two-phase biocatalysis is limited by mass transport, a limitation that can be overcome with the use of microfluidic systems. Although, two-phase biocatalysis in laminar flow systems has been extensively studied, microfluidic droplets have been primarily used for enzyme screening. In this Perspective, we summarize the limited published work on two-phase biocatalysis in microfluidic droplets and discuss the limitations, challenges, and future perspectives of this technology.
format	article
author	Lanting Xiang Felix Kaspar Anett Schallmey Iordania Constantinou
author_facet	Lanting Xiang Felix Kaspar Anett Schallmey Iordania Constantinou
author_sort	Lanting Xiang
title	Two-Phase Biocatalysis in Microfluidic Droplets
title_short	Two-Phase Biocatalysis in Microfluidic Droplets
title_full	Two-Phase Biocatalysis in Microfluidic Droplets
title_fullStr	Two-Phase Biocatalysis in Microfluidic Droplets
title_full_unstemmed	Two-Phase Biocatalysis in Microfluidic Droplets
title_sort	two-phase biocatalysis in microfluidic droplets
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bd9d502cd34142598f450fd924f6569a
work_keys_str_mv	AT lantingxiang twophasebiocatalysisinmicrofluidicdroplets AT felixkaspar twophasebiocatalysisinmicrofluidicdroplets AT anettschallmey twophasebiocatalysisinmicrofluidicdroplets AT iordaniaconstantinou twophasebiocatalysisinmicrofluidicdroplets
_version_	1718412859857174528

Two-Phase Biocatalysis in Microfluidic Droplets

Documents similaires