Cell-derived Extracellular Matrix as maintaining Biomaterial for adipogenic differentiation

The extracellular matrix (ECM) naturally surrounds cells in humans, and therefore represents the ideal biomaterial for tissue engineering. ECM from different tissues exhibit different composition and physical characteristics. Thus, ECM provides not only physical support but also contains crucial bio...

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Autores principales:	Kluger Petra J., Nellinger Svenja, Heine Simon, Volz Ann-Cathrin
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2020
Materias:	extracellular matrix adipose-derived stem cells differentiation biomaterial Medicine R
Acceso en línea:	https://doaj.org/article/bd9ee61ff0e648c6b24fbbf0aa8078f3
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spelling	oai:doaj.org-article:bd9ee61ff0e648c6b24fbbf0aa8078f32021-12-05T14:10:42ZCell-derived Extracellular Matrix as maintaining Biomaterial for adipogenic differentiation2364-550410.1515/cdbme-2020-3106https://doaj.org/article/bd9ee61ff0e648c6b24fbbf0aa8078f32020-09-01T00:00:00Zhttps://doi.org/10.1515/cdbme-2020-3106https://doaj.org/toc/2364-5504The extracellular matrix (ECM) naturally surrounds cells in humans, and therefore represents the ideal biomaterial for tissue engineering. ECM from different tissues exhibit different composition and physical characteristics. Thus, ECM provides not only physical support but also contains crucial biochemical signals that influence cell adhesion, morphology, proliferation and differentiation. Next to native ECM from mature tissue, ECM can also be obtained from the in vitro culture of cells. In this study, we aimed to highlight the supporting effect of cell-derived- ECM (cdECM) on adipogenic differentiation. ASCs were seeded on top of cdECM from ASCs (scdECM) or pre-adipocytes (acdECM). The impact of ECM on cellular activity was determined by LDH assay, WST I assay and BrdU assay. A supporting effect of cdECM substrates on adipogenic differentiation was determined by oil red O staining and subsequent quantification. Results revealed no effect of cdECM substrates on cellular activity. Regarding adipogenic differentiation a supporting effect of cdECM substrates was obtained compared to control. With these results, we confirm cdECM as a promising biomaterial for adipose tissue engineering.Kluger Petra J.Nellinger SvenjaHeine SimonVolz Ann-CathrinDe Gruyterarticleextracellular matrixadipose-derived stem cellsdifferentiationbiomaterialMedicineRENCurrent Directions in Biomedical Engineering, Vol 6, Iss 3, Pp 410-413 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	extracellular matrix adipose-derived stem cells differentiation biomaterial Medicine R
spellingShingle	extracellular matrix adipose-derived stem cells differentiation biomaterial Medicine R Kluger Petra J. Nellinger Svenja Heine Simon Volz Ann-Cathrin Cell-derived Extracellular Matrix as maintaining Biomaterial for adipogenic differentiation
description	The extracellular matrix (ECM) naturally surrounds cells in humans, and therefore represents the ideal biomaterial for tissue engineering. ECM from different tissues exhibit different composition and physical characteristics. Thus, ECM provides not only physical support but also contains crucial biochemical signals that influence cell adhesion, morphology, proliferation and differentiation. Next to native ECM from mature tissue, ECM can also be obtained from the in vitro culture of cells. In this study, we aimed to highlight the supporting effect of cell-derived- ECM (cdECM) on adipogenic differentiation. ASCs were seeded on top of cdECM from ASCs (scdECM) or pre-adipocytes (acdECM). The impact of ECM on cellular activity was determined by LDH assay, WST I assay and BrdU assay. A supporting effect of cdECM substrates on adipogenic differentiation was determined by oil red O staining and subsequent quantification. Results revealed no effect of cdECM substrates on cellular activity. Regarding adipogenic differentiation a supporting effect of cdECM substrates was obtained compared to control. With these results, we confirm cdECM as a promising biomaterial for adipose tissue engineering.
format	article
author	Kluger Petra J. Nellinger Svenja Heine Simon Volz Ann-Cathrin
author_facet	Kluger Petra J. Nellinger Svenja Heine Simon Volz Ann-Cathrin
author_sort	Kluger Petra J.
title	Cell-derived Extracellular Matrix as maintaining Biomaterial for adipogenic differentiation
title_short	Cell-derived Extracellular Matrix as maintaining Biomaterial for adipogenic differentiation
title_full	Cell-derived Extracellular Matrix as maintaining Biomaterial for adipogenic differentiation
title_fullStr	Cell-derived Extracellular Matrix as maintaining Biomaterial for adipogenic differentiation
title_full_unstemmed	Cell-derived Extracellular Matrix as maintaining Biomaterial for adipogenic differentiation
title_sort	cell-derived extracellular matrix as maintaining biomaterial for adipogenic differentiation
publisher	De Gruyter
publishDate	2020
url	https://doaj.org/article/bd9ee61ff0e648c6b24fbbf0aa8078f3
work_keys_str_mv	AT klugerpetraj cellderivedextracellularmatrixasmaintainingbiomaterialforadipogenicdifferentiation AT nellingersvenja cellderivedextracellularmatrixasmaintainingbiomaterialforadipogenicdifferentiation AT heinesimon cellderivedextracellularmatrixasmaintainingbiomaterialforadipogenicdifferentiation AT volzanncathrin cellderivedextracellularmatrixasmaintainingbiomaterialforadipogenicdifferentiation
_version_	1718371805436051456

Cell-derived Extracellular Matrix as maintaining Biomaterial for adipogenic differentiation

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