LIVING MICROORGANISM’S STABILYZATION IN BIOMASS BIOTECHNOLOGY AND PLAGUE VACCINE PREPARATION

Over the years, the production release of the plague vaccine is well developed its technology. The technological cycle of production of the preparation consists of regulated steps, however, despite their effectiveness it is necessary to modernize the manufactoring process, for example, solutions for...

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Autores principales:	D. A. Budika, N. V. Abzaeva, S. E. Gostischeva, E. L. Rakitina, G. F. Ivanova, A. A. Fisun
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2016
Materias:	biotechnology vaccine plague live viability immunogenicity thermal stability monoclonal antibodies Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/bda4330bb7c94a9b888a37eb9b68436a
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spelling	oai:doaj.org-article:bda4330bb7c94a9b888a37eb9b68436a2021-11-22T07:09:47ZLIVING MICROORGANISM’S STABILYZATION IN BIOMASS BIOTECHNOLOGY AND PLAGUE VACCINE PREPARATION2220-76192313-739810.15789/2220-7619-2016-1-87-92https://doaj.org/article/bda4330bb7c94a9b888a37eb9b68436a2016-06-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/389https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Over the years, the production release of the plague vaccine is well developed its technology. The technological cycle of production of the preparation consists of regulated steps, however, despite their effectiveness it is necessary to modernize the manufactoring process, for example, solutions for some of the pressing needs of the customers, in particular, small groups of immunization. Our research has focused on obtaining experimental samples plague vaccine smaller compared to the commercial vaccine, the number of doses per vial prepared in a biomass production unit (ACM-Sh) surface by cultivation using all regulated processing steps, except step of combining content two swabs, and then an additional dilution of the cell suspension stabilizer. However, the time information and the subsequent preparation of such a vaccine is excluded us, since biomass is the second flush in quantitative terms is a ready raw material for the preparation of reduced dosage. The benefits of receiving the vaccine reduced the number of doses directly from the biomass of the second flush with the concentration of microbial cells Yersinia pestis EV 20–40 × 109 biotechnology greatly simplify the manufacture of such a preparation. The experimental vaccine series were tested by major regulated parameters: optical concentration, vitality, thermal stability, the loss on drying. In addition, the vaccine was prefabricated with high baseline viability to extreme temperatures (37±1)°C for 24 hours to exclude enough viable microbial cells for subsequent stabilization indicator of viability during storage. It should be noted that all the experimental samples preserved viability index not lower regulated (25%) during the experiment, in contrast to the commercial preparation. To determine the stability of the formulation during storage (over 3 years) was a comparative analysis of the viability of the experimental and commercial lots. To assess post vaccination immune analyzed the immune response to the introduction of a plague vaccine using FACSCalibur flow cytometer, considering that this technology has a high specificity, sensitivity and informativity. With regard to the immunogenic properties, the active component is recorded at a very high level as the white mice, and guinea pigs. Thus, the main biological indicators derived preparations (viability, thermal stability, storage stability) exceed those of commercial analog and provide effective immunological alterations and highly immunogenic in experimental animals.D. A. BudikaN. V. AbzaevaS. E. GostischevaE. L. RakitinaG. F. IvanovaA. A. FisunSankt-Peterburg : NIIÈM imeni Pasteraarticlebiotechnologyvaccine plague liveviabilityimmunogenicitythermal stabilitymonoclonal antibodiesInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 6, Iss 1, Pp 87-92 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	biotechnology vaccine plague live viability immunogenicity thermal stability monoclonal antibodies Infectious and parasitic diseases RC109-216
spellingShingle	biotechnology vaccine plague live viability immunogenicity thermal stability monoclonal antibodies Infectious and parasitic diseases RC109-216 D. A. Budika N. V. Abzaeva S. E. Gostischeva E. L. Rakitina G. F. Ivanova A. A. Fisun LIVING MICROORGANISM’S STABILYZATION IN BIOMASS BIOTECHNOLOGY AND PLAGUE VACCINE PREPARATION
description	Over the years, the production release of the plague vaccine is well developed its technology. The technological cycle of production of the preparation consists of regulated steps, however, despite their effectiveness it is necessary to modernize the manufactoring process, for example, solutions for some of the pressing needs of the customers, in particular, small groups of immunization. Our research has focused on obtaining experimental samples plague vaccine smaller compared to the commercial vaccine, the number of doses per vial prepared in a biomass production unit (ACM-Sh) surface by cultivation using all regulated processing steps, except step of combining content two swabs, and then an additional dilution of the cell suspension stabilizer. However, the time information and the subsequent preparation of such a vaccine is excluded us, since biomass is the second flush in quantitative terms is a ready raw material for the preparation of reduced dosage. The benefits of receiving the vaccine reduced the number of doses directly from the biomass of the second flush with the concentration of microbial cells Yersinia pestis EV 20–40 × 109 biotechnology greatly simplify the manufacture of such a preparation. The experimental vaccine series were tested by major regulated parameters: optical concentration, vitality, thermal stability, the loss on drying. In addition, the vaccine was prefabricated with high baseline viability to extreme temperatures (37±1)°C for 24 hours to exclude enough viable microbial cells for subsequent stabilization indicator of viability during storage. It should be noted that all the experimental samples preserved viability index not lower regulated (25%) during the experiment, in contrast to the commercial preparation. To determine the stability of the formulation during storage (over 3 years) was a comparative analysis of the viability of the experimental and commercial lots. To assess post vaccination immune analyzed the immune response to the introduction of a plague vaccine using FACSCalibur flow cytometer, considering that this technology has a high specificity, sensitivity and informativity. With regard to the immunogenic properties, the active component is recorded at a very high level as the white mice, and guinea pigs. Thus, the main biological indicators derived preparations (viability, thermal stability, storage stability) exceed those of commercial analog and provide effective immunological alterations and highly immunogenic in experimental animals.
format	article
author	D. A. Budika N. V. Abzaeva S. E. Gostischeva E. L. Rakitina G. F. Ivanova A. A. Fisun
author_facet	D. A. Budika N. V. Abzaeva S. E. Gostischeva E. L. Rakitina G. F. Ivanova A. A. Fisun
author_sort	D. A. Budika
title	LIVING MICROORGANISM’S STABILYZATION IN BIOMASS BIOTECHNOLOGY AND PLAGUE VACCINE PREPARATION
title_short	LIVING MICROORGANISM’S STABILYZATION IN BIOMASS BIOTECHNOLOGY AND PLAGUE VACCINE PREPARATION
title_full	LIVING MICROORGANISM’S STABILYZATION IN BIOMASS BIOTECHNOLOGY AND PLAGUE VACCINE PREPARATION
title_fullStr	LIVING MICROORGANISM’S STABILYZATION IN BIOMASS BIOTECHNOLOGY AND PLAGUE VACCINE PREPARATION
title_full_unstemmed	LIVING MICROORGANISM’S STABILYZATION IN BIOMASS BIOTECHNOLOGY AND PLAGUE VACCINE PREPARATION
title_sort	living microorganism’s stabilyzation in biomass biotechnology and plague vaccine preparation
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2016
url	https://doaj.org/article/bda4330bb7c94a9b888a37eb9b68436a
work_keys_str_mv	AT dabudika livingmicroorganismsstabilyzationinbiomassbiotechnologyandplaguevaccinepreparation AT nvabzaeva livingmicroorganismsstabilyzationinbiomassbiotechnologyandplaguevaccinepreparation AT segostischeva livingmicroorganismsstabilyzationinbiomassbiotechnologyandplaguevaccinepreparation AT elrakitina livingmicroorganismsstabilyzationinbiomassbiotechnologyandplaguevaccinepreparation AT gfivanova livingmicroorganismsstabilyzationinbiomassbiotechnologyandplaguevaccinepreparation AT aafisun livingmicroorganismsstabilyzationinbiomassbiotechnologyandplaguevaccinepreparation
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LIVING MICROORGANISM’S STABILYZATION IN BIOMASS BIOTECHNOLOGY AND PLAGUE VACCINE PREPARATION

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