Nuclear factor kappa B activation in cardiomyocytes by serum of children with obstructive sleep apnea syndrome

Abstract Obstructive sleep apnea syndrome (OSA) is associated with cardiovascular morbidity in adults and children. NFκB activity is enhanced in circulating monocytes of adults with OSA, that decreases following positive pressure therapy. OSA children’s serum activates NFκB in a cell line. We hypoth...

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Autores principales:	Aviv D. Goldbart, Meital Gannot, Hen Haddad, Jacob Gopas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bda5dd0b1c3a41ffa6d9adccf358c4b7
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spelling	oai:doaj.org-article:bda5dd0b1c3a41ffa6d9adccf358c4b72021-12-02T13:58:25ZNuclear factor kappa B activation in cardiomyocytes by serum of children with obstructive sleep apnea syndrome10.1038/s41598-020-79187-02045-2322https://doaj.org/article/bda5dd0b1c3a41ffa6d9adccf358c4b72020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79187-0https://doaj.org/toc/2045-2322Abstract Obstructive sleep apnea syndrome (OSA) is associated with cardiovascular morbidity in adults and children. NFκB activity is enhanced in circulating monocytes of adults with OSA, that decreases following positive pressure therapy. OSA children’s serum activates NFκB in a cell line. We hypothesized that OSA children’s serum can activate NFκB in cardiomyocytes (CM) and effect their viability. In order to explore the role played by NFκB in OSA cardiovascular pathophysiology, rat, mouse and human immortalized CM were exposed to human serum drawn from OSA children and matched controls. Increased expression of NFκB classical subunits p65/p50 as well as major morphological changes occurred in cardiomyocytes following OSA’s serum exposure. OSA children’s serum induced NFκB activity as measured by p65 nuclear translocation in immortalized human CM and rat cardiomyocytes as well as dense immunostaining of the nucleus. Trypan blue and XTT assays showed that OSA sera induced CM apoptosis. We conclude that NFκB is systemically activated in cardiomyocytes, who also demonstrate decreased viability and contractility following exposure to OSA serum. It supports the hypothesis NFκB plays a role in the evolution of cardiovascular morbidity in OSA. It may support the search for new therapeutic interventions controlling NFκB activation in OSA.Aviv D. GoldbartMeital GannotHen HaddadJacob GopasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aviv D. Goldbart Meital Gannot Hen Haddad Jacob Gopas Nuclear factor kappa B activation in cardiomyocytes by serum of children with obstructive sleep apnea syndrome
description	Abstract Obstructive sleep apnea syndrome (OSA) is associated with cardiovascular morbidity in adults and children. NFκB activity is enhanced in circulating monocytes of adults with OSA, that decreases following positive pressure therapy. OSA children’s serum activates NFκB in a cell line. We hypothesized that OSA children’s serum can activate NFκB in cardiomyocytes (CM) and effect their viability. In order to explore the role played by NFκB in OSA cardiovascular pathophysiology, rat, mouse and human immortalized CM were exposed to human serum drawn from OSA children and matched controls. Increased expression of NFκB classical subunits p65/p50 as well as major morphological changes occurred in cardiomyocytes following OSA’s serum exposure. OSA children’s serum induced NFκB activity as measured by p65 nuclear translocation in immortalized human CM and rat cardiomyocytes as well as dense immunostaining of the nucleus. Trypan blue and XTT assays showed that OSA sera induced CM apoptosis. We conclude that NFκB is systemically activated in cardiomyocytes, who also demonstrate decreased viability and contractility following exposure to OSA serum. It supports the hypothesis NFκB plays a role in the evolution of cardiovascular morbidity in OSA. It may support the search for new therapeutic interventions controlling NFκB activation in OSA.
format	article
author	Aviv D. Goldbart Meital Gannot Hen Haddad Jacob Gopas
author_facet	Aviv D. Goldbart Meital Gannot Hen Haddad Jacob Gopas
author_sort	Aviv D. Goldbart
title	Nuclear factor kappa B activation in cardiomyocytes by serum of children with obstructive sleep apnea syndrome
title_short	Nuclear factor kappa B activation in cardiomyocytes by serum of children with obstructive sleep apnea syndrome
title_full	Nuclear factor kappa B activation in cardiomyocytes by serum of children with obstructive sleep apnea syndrome
title_fullStr	Nuclear factor kappa B activation in cardiomyocytes by serum of children with obstructive sleep apnea syndrome
title_full_unstemmed	Nuclear factor kappa B activation in cardiomyocytes by serum of children with obstructive sleep apnea syndrome
title_sort	nuclear factor kappa b activation in cardiomyocytes by serum of children with obstructive sleep apnea syndrome
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/bda5dd0b1c3a41ffa6d9adccf358c4b7
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Nuclear factor kappa B activation in cardiomyocytes by serum of children with obstructive sleep apnea syndrome

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