Spatial distribution of glycerophospholipids in the ocular lens.

Knowledge of the spatial distribution of lipids in the intraocular lens is important for understanding the physiology and biochemistry of this unique tissue and for gaining a better insight into the mechanisms underlying diseases of the lens. Following our previous study showing the spatial distribu...

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Autores principales:	Jaroslav Pól, Veronika Vidová, Tuulia Hyötyläinen, Michael Volný, Petr Novák, Martin Strohalm, Risto Kostiainen, Vladimír Havlíček, Susanne K Wiedmer, Juha M Holopainen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bdaa163ea7d749969264782f72ba9db3
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spelling	oai:doaj.org-article:bdaa163ea7d749969264782f72ba9db32021-11-18T06:54:52ZSpatial distribution of glycerophospholipids in the ocular lens.1932-620310.1371/journal.pone.0019441https://doaj.org/article/bdaa163ea7d749969264782f72ba9db32011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21559377/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Knowledge of the spatial distribution of lipids in the intraocular lens is important for understanding the physiology and biochemistry of this unique tissue and for gaining a better insight into the mechanisms underlying diseases of the lens. Following our previous study showing the spatial distribution of sphingolipids in the porcine lens, the current study used ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOFMS) to provide the whole lipidome of porcine lens and these studies were supplemented by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) of the lens using ultra-high resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) to determine the spatial distribution of glycerophospholipids. Altogether 172 lipid species were identified with high confidence and their concentration was determined. Sphingomyelins, phosphatidylcholines, and phosphatidylethanolamines were the most abundant lipid classes. We then determined the spatial and concentration-dependent distributions of 20 phosphatidylcholines, 6 phosphatidylethanolamines, and 4 phosphatidic acids. Based on the planar molecular images of the lipids, we report the organization of fiber cell membranes within the ocular lens and suggest roles for these lipids in normal and diseased lenses.Jaroslav PólVeronika VidováTuulia HyötyläinenMichael VolnýPetr NovákMartin StrohalmRisto KostiainenVladimír HavlíčekSusanne K WiedmerJuha M HolopainenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e19441 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jaroslav Pól Veronika Vidová Tuulia Hyötyläinen Michael Volný Petr Novák Martin Strohalm Risto Kostiainen Vladimír Havlíček Susanne K Wiedmer Juha M Holopainen Spatial distribution of glycerophospholipids in the ocular lens.
description	Knowledge of the spatial distribution of lipids in the intraocular lens is important for understanding the physiology and biochemistry of this unique tissue and for gaining a better insight into the mechanisms underlying diseases of the lens. Following our previous study showing the spatial distribution of sphingolipids in the porcine lens, the current study used ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOFMS) to provide the whole lipidome of porcine lens and these studies were supplemented by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) of the lens using ultra-high resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) to determine the spatial distribution of glycerophospholipids. Altogether 172 lipid species were identified with high confidence and their concentration was determined. Sphingomyelins, phosphatidylcholines, and phosphatidylethanolamines were the most abundant lipid classes. We then determined the spatial and concentration-dependent distributions of 20 phosphatidylcholines, 6 phosphatidylethanolamines, and 4 phosphatidic acids. Based on the planar molecular images of the lipids, we report the organization of fiber cell membranes within the ocular lens and suggest roles for these lipids in normal and diseased lenses.
format	article
author	Jaroslav Pól Veronika Vidová Tuulia Hyötyläinen Michael Volný Petr Novák Martin Strohalm Risto Kostiainen Vladimír Havlíček Susanne K Wiedmer Juha M Holopainen
author_facet	Jaroslav Pól Veronika Vidová Tuulia Hyötyläinen Michael Volný Petr Novák Martin Strohalm Risto Kostiainen Vladimír Havlíček Susanne K Wiedmer Juha M Holopainen
author_sort	Jaroslav Pól
title	Spatial distribution of glycerophospholipids in the ocular lens.
title_short	Spatial distribution of glycerophospholipids in the ocular lens.
title_full	Spatial distribution of glycerophospholipids in the ocular lens.
title_fullStr	Spatial distribution of glycerophospholipids in the ocular lens.
title_full_unstemmed	Spatial distribution of glycerophospholipids in the ocular lens.
title_sort	spatial distribution of glycerophospholipids in the ocular lens.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/bdaa163ea7d749969264782f72ba9db3
work_keys_str_mv	AT jaroslavpol spatialdistributionofglycerophospholipidsintheocularlens AT veronikavidova spatialdistributionofglycerophospholipidsintheocularlens AT tuuliahyotylainen spatialdistributionofglycerophospholipidsintheocularlens AT michaelvolny spatialdistributionofglycerophospholipidsintheocularlens AT petrnovak spatialdistributionofglycerophospholipidsintheocularlens AT martinstrohalm spatialdistributionofglycerophospholipidsintheocularlens AT ristokostiainen spatialdistributionofglycerophospholipidsintheocularlens AT vladimirhavlicek spatialdistributionofglycerophospholipidsintheocularlens AT susannekwiedmer spatialdistributionofglycerophospholipidsintheocularlens AT juhamholopainen spatialdistributionofglycerophospholipidsintheocularlens
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Spatial distribution of glycerophospholipids in the ocular lens.

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