Applicability of diatom metabarcoding in the ecological status assessment of Hungarian lotic and soda pan habitats

Diatoms are widely applied in the ecological status assessment of aquatic ecosystems using indices calculated from pollution sensitivity and indicator values of species. Traditional, morphology-based identification of species requires in-depth taxonomic knowledge and expertise. Identifying taxa acco...

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Autores principales:	Mónika Duleba, Angéla Földi, Adrienn Micsinai, Gábor Várbíró, Anita Mohr, Rita Sipos, Gyula Szabó, Krisztina Buczkó, Zsuzsa Trábert, Keve T. Kiss, Tibor Bíró, Edit Vadkerti, Éva Ács
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Diatom Metabarcoding Morphology Ecological status assessment Running waters Soda pans Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/bdabaf9f1f034c278f4aaa981428fe53
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spelling	oai:doaj.org-article:bdabaf9f1f034c278f4aaa981428fe532021-12-01T04:59:15ZApplicability of diatom metabarcoding in the ecological status assessment of Hungarian lotic and soda pan habitats1470-160X10.1016/j.ecolind.2021.108105https://doaj.org/article/bdabaf9f1f034c278f4aaa981428fe532021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1470160X21007706https://doaj.org/toc/1470-160XDiatoms are widely applied in the ecological status assessment of aquatic ecosystems using indices calculated from pollution sensitivity and indicator values of species. Traditional, morphology-based identification of species requires in-depth taxonomic knowledge and expertise. Identifying taxa according to their barcode sequences obtained with high-throughput sequencing (metabarcoding) would be a promising alternative. In this pilot study, we tested the applicability of metabarcoding of benthic diatom assemblages for the ecological status assessment of Hungarian water bodies, comparing its performance to that of morphology-based identification of species. The barcode region of the rbcL gene was investigated in samples from running waters with various trophic states and unique lentic habitats, namely soda pans. For running waters, the Specific Pollution Sensitivity Index (IPS) and for soda pans the H index and the Indice Biologique Diatomées (IBD) were calculated from the morphology- and sequence-based abundances. The ecological quality ratio was determined for running waters. Overall, more infrageneric taxa were found with microscopy than with metabarcoding in the lotic and lentic samples. The correspondence between taxon lists detected by the two methods was relatively low but increased considerably in the case of morphologically dominant taxa. Community composition based on microscopy and DNA sequence analysis showed a significant correlation and was determined by the same main environmental drivers. Morphology-based indices strongly correlated with sequence-based indices. Both aspects indicated the same ecological status class for more than half of the lotic samples. In other cases, a status shift from good to moderate was frequent, a phenomenon that could prove problematic because the Water Framework Directive prescribes intervention for waters with a moderate or worse status. Considering discrepancies between the results obtained with microscopy and metabarcoding, using both methods in parallel could be proposed until the reference database has been suitably updated.Mónika DulebaAngéla FöldiAdrienn MicsinaiGábor VárbíróAnita MohrRita SiposGyula SzabóKrisztina BuczkóZsuzsa TrábertKeve T. KissTibor BíróEdit VadkertiÉva ÁcsElsevierarticleDiatomMetabarcodingMorphologyEcological status assessmentRunning watersSoda pansEcologyQH540-549.5ENEcological Indicators, Vol 130, Iss , Pp 108105- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Diatom Metabarcoding Morphology Ecological status assessment Running waters Soda pans Ecology QH540-549.5
spellingShingle	Diatom Metabarcoding Morphology Ecological status assessment Running waters Soda pans Ecology QH540-549.5 Mónika Duleba Angéla Földi Adrienn Micsinai Gábor Várbíró Anita Mohr Rita Sipos Gyula Szabó Krisztina Buczkó Zsuzsa Trábert Keve T. Kiss Tibor Bíró Edit Vadkerti Éva Ács Applicability of diatom metabarcoding in the ecological status assessment of Hungarian lotic and soda pan habitats
description	Diatoms are widely applied in the ecological status assessment of aquatic ecosystems using indices calculated from pollution sensitivity and indicator values of species. Traditional, morphology-based identification of species requires in-depth taxonomic knowledge and expertise. Identifying taxa according to their barcode sequences obtained with high-throughput sequencing (metabarcoding) would be a promising alternative. In this pilot study, we tested the applicability of metabarcoding of benthic diatom assemblages for the ecological status assessment of Hungarian water bodies, comparing its performance to that of morphology-based identification of species. The barcode region of the rbcL gene was investigated in samples from running waters with various trophic states and unique lentic habitats, namely soda pans. For running waters, the Specific Pollution Sensitivity Index (IPS) and for soda pans the H index and the Indice Biologique Diatomées (IBD) were calculated from the morphology- and sequence-based abundances. The ecological quality ratio was determined for running waters. Overall, more infrageneric taxa were found with microscopy than with metabarcoding in the lotic and lentic samples. The correspondence between taxon lists detected by the two methods was relatively low but increased considerably in the case of morphologically dominant taxa. Community composition based on microscopy and DNA sequence analysis showed a significant correlation and was determined by the same main environmental drivers. Morphology-based indices strongly correlated with sequence-based indices. Both aspects indicated the same ecological status class for more than half of the lotic samples. In other cases, a status shift from good to moderate was frequent, a phenomenon that could prove problematic because the Water Framework Directive prescribes intervention for waters with a moderate or worse status. Considering discrepancies between the results obtained with microscopy and metabarcoding, using both methods in parallel could be proposed until the reference database has been suitably updated.
format	article
author	Mónika Duleba Angéla Földi Adrienn Micsinai Gábor Várbíró Anita Mohr Rita Sipos Gyula Szabó Krisztina Buczkó Zsuzsa Trábert Keve T. Kiss Tibor Bíró Edit Vadkerti Éva Ács
author_facet	Mónika Duleba Angéla Földi Adrienn Micsinai Gábor Várbíró Anita Mohr Rita Sipos Gyula Szabó Krisztina Buczkó Zsuzsa Trábert Keve T. Kiss Tibor Bíró Edit Vadkerti Éva Ács
author_sort	Mónika Duleba
title	Applicability of diatom metabarcoding in the ecological status assessment of Hungarian lotic and soda pan habitats
title_short	Applicability of diatom metabarcoding in the ecological status assessment of Hungarian lotic and soda pan habitats
title_full	Applicability of diatom metabarcoding in the ecological status assessment of Hungarian lotic and soda pan habitats
title_fullStr	Applicability of diatom metabarcoding in the ecological status assessment of Hungarian lotic and soda pan habitats
title_full_unstemmed	Applicability of diatom metabarcoding in the ecological status assessment of Hungarian lotic and soda pan habitats
title_sort	applicability of diatom metabarcoding in the ecological status assessment of hungarian lotic and soda pan habitats
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/bdabaf9f1f034c278f4aaa981428fe53
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Applicability of diatom metabarcoding in the ecological status assessment of Hungarian lotic and soda pan habitats

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