Complementing 16S rRNA Gene Amplicon Sequencing with Total Bacterial Load To Infer Absolute Species Concentrations in the Vaginal Microbiome

Complementing 16S rRNA Gene Amplicon Sequencing with Total Bacterial Load To Infer Absolute Species Concentrations in the Vaginal Microbiome

ABSTRACT Whereas 16S rRNA gene amplicon sequencing quantifies relative abundances of bacterial taxa, variation in total bacterial load between samples restricts its ability to reflect absolute concentrations of individual bacterial species. Quantitative PCR (qPCR) can quantify individual species, bu...

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Autores principales: Florencia A. Tettamanti Boshier, Sujatha Srinivasan, Anthony Lopez, Noah G. Hoffman, Sean Proll, David N. Fredricks, Joshua T. Schiffer
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2020
Materias:
applied microbiology
gene amplicon sequencing
quantitative PCR
vaginal microbiome
Microbiology
QR1-502
Acceso en línea:https://doaj.org/article/bdad9b0c0bff482d853a48dc2e675993
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