Gene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn’s Disease

Introduction: The inflammatory process in Crohn’s disease (CD) is closely associated with the formation of reactive oxygen species. Antioxidant enzymes can play an important role in the outcome of CD and may influence postoperative recurrence in these patients. The aim of our study was to evaluate g...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Otakar Sotona, Eva Peterová, Július Örhalmi, Tomáš Dušek, Alena Mrkvicová, Veronika Knoblochová, Petr Lochman, Ondřej Malý, Jiří Páral, Jan Bureš
Formato:	article
Lenguaje:	EN
Publicado:	Karolinum Press 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/bdb803adbf96474e884589ea20ed3520
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bdb803adbf96474e884589ea20ed3520
record_format	dspace
spelling	oai:doaj.org-article:bdb803adbf96474e884589ea20ed35202021-11-22T09:31:01ZGene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn’s Disease1805-96941211-428610.14712/18059694.2021.26https://doaj.org/article/bdb803adbf96474e884589ea20ed35202021-11-01T00:00:00Zhttps://actamedica.lfhk.cuni.cz/64/3/0153/https://doaj.org/toc/1211-4286https://doaj.org/toc/1805-9694Introduction: The inflammatory process in Crohn’s disease (CD) is closely associated with the formation of reactive oxygen species. Antioxidant enzymes can play an important role in the outcome of CD and may influence postoperative recurrence in these patients. The aim of our study was to evaluate gene expression of intracellular antioxidant enzymes in surgically resected intestinal specimens of patients with CD, both in macroscopically normal and in inflamed tissue. Methods: A total of 28 patients referred for elective bowel resection were enrolled in the study. Full-thickness small intestinal specimens were investigated. Gene expression of antioxidant enzymes – superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione reductase (GSR) – was evaluated both in macroscopically normal and inflamed samples. Results: There were significantly lower levels of SOD1 mRNA (p = 0.007) and GSR mRNA (p = 0.027) in inflamed tissue compared to macroscopically normal areas. No significant differences were found between affected and non-affected intestinal segments in mRNA for SOD2, SOD3 and GPX. Conclusions: Our pilot data clearly showed that the gene expression of major antioxidant enzymes is not a uniform mechanism in the pathogenesis of Crohn’s disease. Topically decreased gene expression of SOD1 and GSR might facilitate the segmental tissue injury caused by reactive oxygen species.Otakar SotonaEva PeterováJúlius ÖrhalmiTomáš DušekAlena MrkvicováVeronika KnoblochováPetr LochmanOndřej MalýJiří PáralJan BurešKarolinum PressarticleMedicineRENActa Medica, Vol 64, Iss 3, Pp 153-157 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Otakar Sotona Eva Peterová Július Örhalmi Tomáš Dušek Alena Mrkvicová Veronika Knoblochová Petr Lochman Ondřej Malý Jiří Páral Jan Bureš Gene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn’s Disease
description	Introduction: The inflammatory process in Crohn’s disease (CD) is closely associated with the formation of reactive oxygen species. Antioxidant enzymes can play an important role in the outcome of CD and may influence postoperative recurrence in these patients. The aim of our study was to evaluate gene expression of intracellular antioxidant enzymes in surgically resected intestinal specimens of patients with CD, both in macroscopically normal and in inflamed tissue. Methods: A total of 28 patients referred for elective bowel resection were enrolled in the study. Full-thickness small intestinal specimens were investigated. Gene expression of antioxidant enzymes – superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione reductase (GSR) – was evaluated both in macroscopically normal and inflamed samples. Results: There were significantly lower levels of SOD1 mRNA (p = 0.007) and GSR mRNA (p = 0.027) in inflamed tissue compared to macroscopically normal areas. No significant differences were found between affected and non-affected intestinal segments in mRNA for SOD2, SOD3 and GPX. Conclusions: Our pilot data clearly showed that the gene expression of major antioxidant enzymes is not a uniform mechanism in the pathogenesis of Crohn’s disease. Topically decreased gene expression of SOD1 and GSR might facilitate the segmental tissue injury caused by reactive oxygen species.
format	article
author	Otakar Sotona Eva Peterová Július Örhalmi Tomáš Dušek Alena Mrkvicová Veronika Knoblochová Petr Lochman Ondřej Malý Jiří Páral Jan Bureš
author_facet	Otakar Sotona Eva Peterová Július Örhalmi Tomáš Dušek Alena Mrkvicová Veronika Knoblochová Petr Lochman Ondřej Malý Jiří Páral Jan Bureš
author_sort	Otakar Sotona
title	Gene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn’s Disease
title_short	Gene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn’s Disease
title_full	Gene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn’s Disease
title_fullStr	Gene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn’s Disease
title_full_unstemmed	Gene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn’s Disease
title_sort	gene expression of antioxidant enzymes in the resected intestine in crohn’s disease
publisher	Karolinum Press
publishDate	2021
url	https://doaj.org/article/bdb803adbf96474e884589ea20ed3520
work_keys_str_mv	AT otakarsotona geneexpressionofantioxidantenzymesintheresectedintestineincrohnsdisease AT evapeterova geneexpressionofantioxidantenzymesintheresectedintestineincrohnsdisease AT juliusorhalmi geneexpressionofantioxidantenzymesintheresectedintestineincrohnsdisease AT tomasdusek geneexpressionofantioxidantenzymesintheresectedintestineincrohnsdisease AT alenamrkvicova geneexpressionofantioxidantenzymesintheresectedintestineincrohnsdisease AT veronikaknoblochova geneexpressionofantioxidantenzymesintheresectedintestineincrohnsdisease AT petrlochman geneexpressionofantioxidantenzymesintheresectedintestineincrohnsdisease AT ondrejmaly geneexpressionofantioxidantenzymesintheresectedintestineincrohnsdisease AT jiriparal geneexpressionofantioxidantenzymesintheresectedintestineincrohnsdisease AT janbures geneexpressionofantioxidantenzymesintheresectedintestineincrohnsdisease
_version_	1718417818002653184

Gene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn’s Disease

Ejemplares similares