Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake

Johanna M Poller,1,2 Jan Zaloga,1 Eveline Schreiber,1 Harald Unterweger,1 Christina Janko,1 Patricia Radon,3 Dietmar Eberbeck,3 Lutz Trahms,3 Christoph Alexiou,1 Ralf P Friedrich1 1Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology & Nanomedicine (SE...

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Autores principales:	Poller JM, Zaloga J, Schreiber E, Unterweger H, Janko C, Radon P, Eberbeck D, Trahms L, Alexiou C, Friedrich RP
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	breast cancer superparamagnetic iron oxide nanoparticles cellular SPION uptake toxicity flow cytometry Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bdb8c2a535b24457a56b573277729452
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spelling	oai:doaj.org-article:bdb8c2a535b24457a56b5732777294522021-12-02T06:37:58ZSelection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake1178-2013https://doaj.org/article/bdb8c2a535b24457a56b5732777294522017-04-01T00:00:00Zhttps://www.dovepress.com/selection-of-potential-iron-oxide-nanoparticles-for-breast-cancer-trea-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Johanna M Poller,1,2 Jan Zaloga,1 Eveline Schreiber,1 Harald Unterweger,1 Christina Janko,1 Patricia Radon,3 Dietmar Eberbeck,3 Lutz Trahms,3 Christoph Alexiou,1 Ralf P Friedrich1 1Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology & Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, Universitätsklinikum Erlangen, Erlangen, 2Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 3Physikalisch-Technische Bundesanstalt Braunschweig und Berlin, Berlin, Germany Abstract: Superparamagnetic iron oxide nanoparticles (SPIONs) are promising tools for the treatment of different diseases. Their magnetic properties enable therapies involving magnetic drug targeting (MDT), hyperthermia or imaging. Depending on the intended treatment, specific characteristics of SPIONs are required. While particles used for imaging should circulate for extended periods of time in the vascular system, SPIONs intended for MDT or hyperthermia should be accumulated in the target area to come into close proximity of, or to be incorporated into, specific tumor cells. In this study, we determined the impact of several accurately characterized SPION types varying in size, zeta potential and surface coating on various human breast cancer cell lines and endothelial cells to identify the most suitable particle for future breast cancer therapy. We analyzed cellular SPION uptake, magnetic properties, cell proliferation and toxicity using atomic emission spectroscopy, magnetic susceptometry, flow cytometry and microscopy. The results demonstrated that treatment with dextran-coated SPIONs (SPIONDex) and lauric acid-coated SPIONs (SPIONLA) with an additional protein corona formed by human serum albumin (SPIONLA-HSA) resulted in very moderate particle uptake and low cytotoxicity, whereas SPIONLA had in part much stronger effects on cellular uptake and cellular toxicity. In summary, our data show significant dose-dependent and particle type-related response differences between various breast cancer and endothelial cells, indicating the utility of these particle types for distinct medical applications. Keywords: breast cancer, superparamagnetic iron oxide nanoparticles, cellular SPION uptake, toxicity, flow cytometryPoller JMZaloga JSchreiber EUnterweger HJanko CRadon PEberbeck DTrahms LAlexiou CFriedrich RPDove Medical Pressarticlebreast cancersuperparamagnetic iron oxide nanoparticlescellular SPION uptaketoxicityflow cytometryMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 3207-3220 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	breast cancer superparamagnetic iron oxide nanoparticles cellular SPION uptake toxicity flow cytometry Medicine (General) R5-920
spellingShingle	breast cancer superparamagnetic iron oxide nanoparticles cellular SPION uptake toxicity flow cytometry Medicine (General) R5-920 Poller JM Zaloga J Schreiber E Unterweger H Janko C Radon P Eberbeck D Trahms L Alexiou C Friedrich RP Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake
description	Johanna M Poller,1,2 Jan Zaloga,1 Eveline Schreiber,1 Harald Unterweger,1 Christina Janko,1 Patricia Radon,3 Dietmar Eberbeck,3 Lutz Trahms,3 Christoph Alexiou,1 Ralf P Friedrich1 1Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology & Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, Universitätsklinikum Erlangen, Erlangen, 2Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 3Physikalisch-Technische Bundesanstalt Braunschweig und Berlin, Berlin, Germany Abstract: Superparamagnetic iron oxide nanoparticles (SPIONs) are promising tools for the treatment of different diseases. Their magnetic properties enable therapies involving magnetic drug targeting (MDT), hyperthermia or imaging. Depending on the intended treatment, specific characteristics of SPIONs are required. While particles used for imaging should circulate for extended periods of time in the vascular system, SPIONs intended for MDT or hyperthermia should be accumulated in the target area to come into close proximity of, or to be incorporated into, specific tumor cells. In this study, we determined the impact of several accurately characterized SPION types varying in size, zeta potential and surface coating on various human breast cancer cell lines and endothelial cells to identify the most suitable particle for future breast cancer therapy. We analyzed cellular SPION uptake, magnetic properties, cell proliferation and toxicity using atomic emission spectroscopy, magnetic susceptometry, flow cytometry and microscopy. The results demonstrated that treatment with dextran-coated SPIONs (SPIONDex) and lauric acid-coated SPIONs (SPIONLA) with an additional protein corona formed by human serum albumin (SPIONLA-HSA) resulted in very moderate particle uptake and low cytotoxicity, whereas SPIONLA had in part much stronger effects on cellular uptake and cellular toxicity. In summary, our data show significant dose-dependent and particle type-related response differences between various breast cancer and endothelial cells, indicating the utility of these particle types for distinct medical applications. Keywords: breast cancer, superparamagnetic iron oxide nanoparticles, cellular SPION uptake, toxicity, flow cytometry
format	article
author	Poller JM Zaloga J Schreiber E Unterweger H Janko C Radon P Eberbeck D Trahms L Alexiou C Friedrich RP
author_facet	Poller JM Zaloga J Schreiber E Unterweger H Janko C Radon P Eberbeck D Trahms L Alexiou C Friedrich RP
author_sort	Poller JM
title	Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake
title_short	Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake
title_full	Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake
title_fullStr	Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake
title_full_unstemmed	Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake
title_sort	selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/bdb8c2a535b24457a56b573277729452
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Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake

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